Abstract:
:Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed this method to structurally characterize GAGs in tissues, including laser-dissected layers of skin and pathological specimens. This method enables the use of the archival paraffin-embedded material for detailed (structural) analysis of GAGs.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
van Wijk XM,Vallen MJ,van de Westerlo EM,Oosterhof A,Hao W,Versteeg EM,Raben J,Wismans RG,Smetsers TF,Dijkman HB,Schalkwijk J,van Kuppevelt THdoi
10.1093/glycob/cws119subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1666-72issue
12eissn
0959-6658issn
1460-2423pii
cws119journal_volume
22pub_type
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