Subunit-specific sulphation of oligosaccharides relating to charge-heterogeneity in porcine lutrophin isoforms.

abstract：:The product of p53-induced gene 1 is a member of the galectin family, i.e., galectin-7 (Gal-7). To move beyond structural data by X-ray diffraction, we initiated the study of the lectin by nuclear magnetic resonance (NMR) and circular dichroism spectroscopies, and molecular dynamics (MD) simulations. In concert, our r...

journal_title：Glycobiology

authors： Ermakova E,Miller MC,Nesmelova IV,López-Merino L,Berbís MA,Nesmelov Y,Tkachev YV,Lagartera L,Daragan VA,André S,Cañada FJ,Jiménez-Barbero J,Solís D,Gabius HJ,Mayo KH

更新日期：2013-05-01 00:00:00

abstract：:We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...

journal_title：Glycobiology

authors： Chan AL,Morris HR,Panico M,Etienne AT,Rogers ME,Gaffney P,Creighton-Kempsford L,Dell A

更新日期：1991-03-01 00:00:00

abstract：:Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...

journal_title：Glycobiology

authors： Kaida K,Ariga T,Yu RK

更新日期：2009-07-01 00:00:00

abstract：:The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...

journal_title：Glycobiology

authors： Yannariello-Brown J,Zhou B,Weigel PH

更新日期：1997-02-01 00:00:00

abstract：:O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltra...

journal_title：Glycobiology

authors： Chong SL,Virkki L,Maaheimo H,Juvonen M,Derba-Maceluch M,Koutaniemi S,Roach M,Sundberg B,Tuomainen P,Mellerowicz EJ,Tenkanen M

更新日期：2014-06-01 00:00:00

abstract：:N-Acetylglucosaminlyltransferase-V (GnT-V) synthesizes GlcNAcbeta1,6Man branched N-glycans both in vitro and in vivo. A paralog, GnT-Vb (or GnT-IX), has also been shown to synthesize both GlcNAcbeta1,6Man branched N- and O-glycans. GnT-V is expressed in most human and rodent tissues while GnT-Vb expression is limited ...

journal_title：Glycobiology

authors： Alvarez-Manilla G,Troupe K,Fleming M,Martinez-Uribe E,Pierce M

更新日期：2010-02-01 00:00:00

abstract：:In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...

journal_title：Glycobiology

authors： Mast SW,Diekman K,Karaveg K,Davis A,Sifers RN,Moremen KW

更新日期：2005-04-01 00:00:00

abstract：:CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...

journal_title：Glycobiology

authors： Lifely MR,Hale C,Boyce S,Keen MJ,Phillips J

更新日期：1995-12-01 00:00:00

abstract：:Heat stress on Madin-Darby canine kidney cells increased ceramide content to 187% at 40 degrees C for 24 h, and the de novo synthesis from serine increased to 146%. Glucosylceramide (GlcCer) and galactosylceramide (GalCer) synthesis from ceramide, the first glycosylation step of sphingolipid metabolism in kidney cells...

journal_title：Glycobiology

authors： Niimura Y,Moue T,Takahashi N,Nagai K

更新日期：2010-06-01 00:00:00

abstract：:Cryptococcus neoformans, an opportunistic fungal pathogen, produces a glycan capsule to evade the immune system during infection. This definitive virulence factor is composed mainly of complex polysaccharides, which are made in the secretory pathway by reactions that utilize activated nucleotide sugar precursors. Alth...

journal_title：Glycobiology

authors： Li LX,Ashikov A,Liu H,Griffith CL,Bakker H,Doering TL

更新日期：2017-01-01 00:00:00

abstract：:Two kinds of molecules, one recognizing the sialo-/asialo-N-acetyllactosamine structures and the other recognizing the Lewis X structure in a divalent cation-independent manner, were detected on the head of boar sperm prepared from cauda epididymis by fluorescence-labeled or biotinylated dextran-based multivalent olig...

journal_title：Glycobiology

authors： Yoshitani N,Mori E,Takasaki S

更新日期：2001-04-01 00:00:00

abstract：:The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...

journal_title：Glycobiology

authors： Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

更新日期：2008-12-01 00:00:00

abstract：:The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...

journal_title：Glycobiology

authors： Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

更新日期：1999-07-01 00:00:00

abstract：:Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...

journal_title：Glycobiology

authors： Raman R,Venkataraman M,Ramakrishnan S,Lang W,Raguram S,Sasisekharan R

更新日期：2006-05-01 00:00:00

abstract：:In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. The truncated glycosylation exposes cryptic peptide epitopes that can be recognized by antibodies. Since these immunogenic regions are cancer specific, they represent ideal targets for therapeutic antibodies. We investigated ...

journal_title：Glycobiology

authors： Movahedin M,Brooks TM,Supekar NT,Gokanapudi N,Boons GJ,Brooks CL

更新日期：2017-07-01 00:00:00

abstract：:Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...

journal_title：Glycobiology

authors： Vionnet J,Vann WF

更新日期：2007-07-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...

journal_title：Glycobiology

authors： Fukuta K,Abe R,Yokomatsu T,Kono N,Asanagi M,Omae F,Minowa MT,Takeuchi M,Makino T

更新日期：2000-04-01 00:00:00

abstract：:We have elucidated the carbohydrate-binding profile of a non-monosaccharide-binding lectin named Eucheuma serra lectin (ESA)-2 from the red alga Eucheuma serra using a lectin-immobilized column and a centrifugal ultrafiltration-high performance liquid chromatography method with a variety of fluorescence-labeled oligos...

journal_title：Glycobiology

authors： Hori K,Sato Y,Ito K,Fujiwara Y,Iwamoto Y,Makino H,Kawakubo A

更新日期：2007-05-01 00:00:00

abstract：:Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. Here we describe the construction of a stable recom...

journal_title：Glycobiology

authors： Mattila P,Räbinä J,Hortling S,Helin J,Renkonen R

更新日期：2000-10-01 00:00:00

abstract：:Core alpha1,6-fucosylation is a conserved feature of animal N-linked oligosaccharides being present in both invertebrates and vertebrates. To prove that the enzymatic basis for this modification is also evolutionarily conserved, cDNAs encoding the catalytic regions of the predicted Caenorhabditis elegans and Drosophil...

journal_title：Glycobiology

authors： Paschinger K,Staudacher E,Stemmer U,Fabini G,Wilson IB

更新日期：2005-05-01 00:00:00

abstract：:Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...

journal_title：Glycobiology

authors： Pashov A,MacLeod S,Saha R,Perry M,VanCott TC,Kieber-Emmons T

更新日期：2005-10-01 00:00:00

abstract：:To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...

journal_title：Glycobiology

authors： Coppa GV,Gabrielli O,Buzzega D,Zampini L,Galeazzi T,Maccari F,Bertino E,Volpi N

更新日期：2011-03-01 00:00:00

abstract：:We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...

journal_title：Glycobiology

authors： Itoh M,Fukumoto S,Baba N,Kuga Y,Mizuno A,Furukawa K

更新日期：1999-11-01 00:00:00

abstract：:Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...

journal_title：Glycobiology

authors： Haxaire K,Braccini I,Milas M,Rinaudo M,Pérez S

更新日期：2000-06-01 00:00:00

abstract：:Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that G...

journal_title：Glycobiology

authors： Cerri DG,Rodrigues LC,Stowell SR,Araujo DD,Coelho MC,Oliveira SR,Bizario JC,Cummings RD,Dias-Baruffi M,Costa MC

更新日期：2008-11-01 00:00:00

abstract：:Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respective...

journal_title：Glycobiology

authors： Weisshaar G,Hiyama J,Renwick AG

更新日期：1991-09-01 00:00:00

abstract：:Knowledge of the identity, synthesis and secretion of beta-galactoside-binding lectins by leukocytes is of importance because lactosaminoglycans present at the leukocyte cell surface may be physiologically significant lectin receptors that could mediate autocrine or paracrine functions and/or cell adhesion. This paper...

journal_title：Glycobiology

authors： Sharma A,DiCioccio RA,Allen HJ

更新日期：1992-08-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...

journal_title：Glycobiology

authors： Langsdorf A,Schumacher V,Shi X,Tran T,Zaia J,Jain S,Taglienti M,Kreidberg JA,Fine A,Ai X

更新日期：2011-02-01 00:00:00