Abstract:
:The product of p53-induced gene 1 is a member of the galectin family, i.e., galectin-7 (Gal-7). To move beyond structural data by X-ray diffraction, we initiated the study of the lectin by nuclear magnetic resonance (NMR) and circular dichroism spectroscopies, and molecular dynamics (MD) simulations. In concert, our results indicate that lactose binding to human Gal-7 induces long-range effects (minor conformational shifts and changes in structural dynamics) throughout the protein that result in stabilization of the dimer state, with evidence for positive cooperativity. Monte Carlo fits of (15)N-Gal-7 HSQC titrations with lactose using a two-site model yield K1 = 0.9 ± 0.6 × 10(3) M(-1) and K2 = 3.4 ± 0.8 × 10(3) M(-1). Ligand binding-induced stabilization of the Gal-7 dimer was supported by several lines of evidence: MD-based calculations of interaction energies between ligand-loaded and ligand-free states, gel filtration data and hetero-FRET spectroscopy that indicate a highly reduced tendency for dimer dissociation in the presence of lactose, CD-based thermal denaturation showing that the transition temperature of the lectin is significantly increased in the presence of lactose, and saturation transfer difference (STD) NMR using a molecular probe of the monomer state whose presence is diminished in the presence of lactose. MD simulations with the half-loaded ligand-bound state also provided insight into how allosteric signaling may occur. Overall, our results reveal long-range effects on Gal-7 structure and dynamics, which factor into entropic contributions to ligand binding and allow further comparisons with other members of the galectin family.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Ermakova E,Miller MC,Nesmelova IV,López-Merino L,Berbís MA,Nesmelov Y,Tkachev YV,Lagartera L,Daragan VA,André S,Cañada FJ,Jiménez-Barbero J,Solís D,Gabius HJ,Mayo KHdoi
10.1093/glycob/cwt005subject
Has Abstractpub_date
2013-05-01 00:00:00pages
508-23issue
5eissn
0959-6658issn
1460-2423pii
cwt005journal_volume
23pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww019
更新日期:2016-07-01 00:00:00
abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf059
更新日期:2002-08-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium responsible for serious enteric infections that occur mainly in the terminal ileum and colon. High interest in Shigella, as a human pathogen, is driven by its antibiotic resistance and the necessity to develop a vaccine against its infections. Vaccines of the last generat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm140
更新日期:2008-03-01 00:00:00
abstract::We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demons...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm087
更新日期:2007-11-01 00:00:00
abstract::We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.3.163
更新日期:2002-03-01 00:00:00
abstract::Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition and cellular processes underlying embryonic development are still not understood. We analyze the possible link with the mitogen-activated prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg103
更新日期:2003-10-01 00:00:00
abstract::Cell-surface glycans are functional docking sites for tissue lectins such as the members of the galectin family. This interaction triggers a wide variety of responses; hence, there is a keen interest in defining its structural features. Toward this aim, we have used enzyme-linked lectinosorbent (ELLSA) and inhibition ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj102
更新日期:2006-06-01 00:00:00
abstract::Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu020
更新日期:2014-06-01 00:00:00
abstract::There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwv102
更新日期:2016-03-01 00:00:00
abstract::Serglycin with a green fluorescent protein tag (SG-GFP) expressed in epithelial Madin-Darby canine kidney cells is secreted mainly (85%) into the apical medium, but the glycosaminoglycan (GAG) chains on the SG-GFP protein core secreted basolaterally (15%) carry most of the sulfate added during biosynthesis (Tveit et a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj075
更新日期:2006-04-01 00:00:00
abstract::Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi084
更新日期:2005-10-01 00:00:00
abstract::The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm058
更新日期:2007-08-01 00:00:00
abstract::Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwx056
更新日期:2017-09-01 00:00:00
abstract::From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.5.517
更新日期:1998-05-01 00:00:00
abstract::A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg014
更新日期:2003-02-01 00:00:00
abstract::To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq164
更新日期:2011-03-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu027
更新日期:2014-07-01 00:00:00
abstract::Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz058
更新日期:2019-10-21 00:00:00
abstract::Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws086
更新日期:2012-08-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00
abstract::Shigella flexneri is the major human pathogen causing shigellosis. O-antigens of all S. flexneri serotypes (except for serotype 6) share the →2)-α-l-Rhap(III)-(1 → 2)-α-l-Rhap(II)-(1 → 3)-α-l-Rhap(I)-(1 → 3)-β-d-GlcpNAc-(1→ basic O-unit, whereas differences between the serotypes are conferred by phage-encoded glucosyl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws222
更新日期:2013-04-01 00:00:00
abstract::The synthesis of the common and well-documented Siaalpha 2,6 to Galbeta 1,4GlcNAc structure (Sia6LacNAc) is principally mediated by the sialyltransferase ST6Gal I, which is particularly highly expressed in liver, lactating mammary gland, intestinal epithelia of newborn animals, and B cells. Multiple independent promot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg066
更新日期:2003-08-01 00:00:00
abstract::We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.2.173
更新日期:1991-03-01 00:00:00
abstract::alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.12.1283
更新日期:2000-12-01 00:00:00
abstract::Protein O-fucosyltransferase 1 (Pofut1) and protein O-fucosyltransferase 2 (Pofut2) add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively. Glycan chain elongation past O-fucose can occur to yield a tetrasa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws140
更新日期:2013-02-01 00:00:00
abstract::The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for puta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq059
更新日期:2010-08-01 00:00:00
abstract::In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. The truncated glycosylation exposes cryptic peptide epitopes that can be recognized by antibodies. Since these immunogenic regions are cancer specific, they represent ideal targets for therapeutic antibodies. We investigated ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww131
更新日期:2017-07-01 00:00:00
abstract::Recombinant human erythropoietin (rHuEPO) is used abundantly in the clinic to stimulate red blood cell growth in anaemic patients. The efficacy of the drug depends strongly on the extent of sialylation of its carbohydrate moiety. Prompted by conflicting literature reports on the issue, we reinvestigated the structures...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.227
更新日期:1994-04-01 00:00:00
abstract::Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr172
更新日期:2012-04-01 00:00:00