Successive glycosyltransfer of sialic acid by Escherichia coli K92 polysialyltransferase in elongation of oligosialic acceptors.

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:Beta-1,2-linked mannosides from Candida albicans phosphopeptidomannan (PPM) bind to macrophages through a receptor independent from the macrophage alpha-linked mannose receptor and stimulate these cells to secrete immune mediators. Anti-beta-1,2-linked mannoside but not anti-alpha-linked mannoside antibodies produced ...

journal_title：Glycobiology

authors： Jouault T,Fradin C,Dzierszinski F,Borg-Von-Zepelin M,Tomavo S,Corman R,Trinel PA,Kerckaert JP,Poulain D

更新日期：2001-08-01 00:00:00

abstract：:The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...

journal_title：Glycobiology

authors： Cinelli LP,Castro MO,Santos LL,Garcia CR,Vilela-Silva AC,Mourão PA

更新日期：2007-08-01 00:00:00

abstract：:The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...

journal_title：Glycobiology

authors： Nielsen TC,Meikle PJ,Hopwood JJ,Fuller M

更新日期：2008-12-01 00:00:00

abstract：:N-acetylglucosaminyltransferase V, encoded by the Mgat5 gene, plays an important regulatory role in the synthesis of complex N-glycans, including the Lewis antigens. The elevated expression of this enzyme is regulated during development and is highly associated with cellular transformation and malignancy. In addition,...

journal_title：Glycobiology

authors： Alverez K,Haswell C,St Clair M,Perng GS,Shorebah M,Pierce M,Fregien N

更新日期：2002-07-01 00:00:00

abstract：:β-Glucans possess broad immunomodulatory properties, including activation of innate immune functions such as oxidative burst activity. The differential roles of complement receptor type 3 (CR3) and Dectin-1, the known β-glucan receptors, and their associated signaling pathways in the generation of oxidative burst indu...

journal_title：Glycobiology

authors： Bose N,Wurst LR,Chan AS,Dudney CM,LeRoux ML,Danielson ME,Will PM,Nodland SE,Patchen ML,Dalle Lucca JJ,Lebeda FJ,Vasilakos JP

更新日期：2014-04-01 00:00:00

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00

abstract：:Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...

journal_title：Glycobiology

authors： Hileman RE,Smith AE,Toida T,Linhardt RJ

更新日期：1997-03-01 00:00:00

abstract：:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...

journal_title：Glycobiology

authors： de Haan N,Falck D,Wuhrer M

更新日期：2020-03-20 00:00:00

abstract：:Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition and cellular processes underlying embryonic development are still not understood. We analyze the possible link with the mitogen-activated prot...

journal_title：Glycobiology

authors： Snaar-Jagalska BE,Krens SF,Robina I,Wang LX,Spaink HP

更新日期：2003-10-01 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...

journal_title：Glycobiology

authors： Kotani M,Kawashima I,Ozawa H,Terashima T,Tai T

更新日期：1993-04-01 00:00:00

abstract：:In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...

journal_title：Glycobiology

authors： Angulo I,Acebrón I,de las Rivas B,Muñoz R,Rodríguez-Crespo I,Menéndez M,García P,Tateno H,Goldstein IJ,Pérez-Agote B,Mancheño JM

更新日期：2011-10-01 00:00:00

abstract：:Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...

journal_title：Glycobiology

authors： Malik RK,Ghurye RR,Lawrence-Watt DJ,Stewart HJ

更新日期：2009-12-01 00:00:00

abstract：:Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...

journal_title：Glycobiology

authors： Fukuta K,Abe R,Yokomatsu T,Kono N,Asanagi M,Omae F,Minowa MT,Takeuchi M,Makino T

更新日期：2000-04-01 00:00:00

abstract：:Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of matu...

journal_title：Glycobiology

authors： Rey-Gallardo A,Escribano C,Delgado-Martín C,Rodriguez-Fernández JL,Gerardy-Schahn R,Rutishauser U,Corbi AL,Vega MA

更新日期：2010-09-01 00:00:00

abstract：:Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...

journal_title：Glycobiology

authors： Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WF

更新日期：2014-02-01 00:00:00

abstract：:Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...

journal_title：Glycobiology

authors： Bencúrová M,Hemmer W,Focke-Tejkl M,Wilson IB,Altmann F

更新日期：2004-05-01 00:00:00

abstract：:The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis. At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels. The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a conce...

journal_title：Glycobiology

authors： Pan YT,Drake RR,Elbein AD

更新日期：1996-06-01 00:00:00

abstract：:We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...

journal_title：Glycobiology

authors： Itoh M,Fukumoto S,Baba N,Kuga Y,Mizuno A,Furukawa K

更新日期：1999-11-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...

journal_title：Glycobiology

authors： Yanagisawa M,Ariga T,Yu RK

更新日期：2006-09-01 00:00:00

abstract：:Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...

journal_title：Glycobiology

authors： Sapay N,Cabannes E,Petitou M,Imberty A

更新日期：2011-09-01 00:00:00

abstract：:The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...

journal_title：Glycobiology

authors： Chen CP,Song SC,Gilboa-Garber N,Chang KS,Wu AM

更新日期：1998-01-01 00:00:00

abstract：:We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...

journal_title：Glycobiology

authors： Kawabe K,Tateyama D,Toyoda H,Kawasaki N,Hashii N,Nakao H,Matsumoto S,Nonaka M,Matsumura H,Hirose Y,Morita A,Katayama M,Sakuma M,Kawasaki N,Furue MK,Kawasaki T

更新日期：2013-03-01 00:00:00

abstract：:The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...

journal_title：Glycobiology

authors： Gagneux P,Varki A

更新日期：1999-08-01 00:00:00

abstract：:It is widely reported that derivatives of sugar moieties can be used to metabolically label cell surface carbohydrates or inhibit a particular glycosylation. However, few studies address the effect of substitution of the cytidylmonophosphate (CMP) portion on sialyltransferase activities. Here we first synthesized 2'-O...

journal_title：Glycobiology

authors： Miyazaki T,Angata K,Seeberger PH,Hindsgaul O,Fukuda M

更新日期：2008-02-01 00:00:00

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

journal_title：Glycobiology

authors： Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

更新日期：1997-12-01 00:00:00

abstract：:Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...

journal_title：Glycobiology

authors： Marino JH,Hoffman M,Meyer M,Miller KS

更新日期：2004-12-01 00:00:00

abstract：:The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...

journal_title：Glycobiology

authors： Kakuda S,Sato Y,Tonoyama Y,Oka S,Kawasaki T

更新日期：2005-02-01 00:00:00