Differential distribution of major gangliosides in rat central nervous system detected by specific monoclonal antibodies.

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branc...

journal_title：Glycobiology

authors： Fontana C,Ramström K,Weintraub A,Widmalm G

更新日期：2013-03-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...

journal_title：Glycobiology

authors： Mbemba E,Gluckman JC,Gattegno L

更新日期：2000-01-01 00:00:00

abstract：:Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glyca...

journal_title：Glycobiology

authors： Li L,Guan W,Zhang G,Wu Z,Yu H,Chen X,Wang PG

更新日期：2020-04-20 00:00:00

abstract：:The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...

journal_title：Glycobiology

authors： Campbell MP,Abrahams JL,Rapp E,Struwe WB,Costello CE,Novotny M,Ranzinger R,York WS,Kolarich D,Rudd PM,Kettner C

更新日期：2019-05-01 00:00:00

abstract：:Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

journal_title：Glycobiology

authors： Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

更新日期：2006-06-01 00:00:00

abstract：:Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...

journal_title：Glycobiology

authors： Grabińska KA,Cui J,Chatterjee A,Guan Z,Raetz CR,Robbins PW,Samuelson J

更新日期：2010-07-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:In this study, we report the first cloning and characterization of a N-acetylneuraminic acid phosphate synthase gene from Drosophila melanogaster, an insect in the protostome lineage. The gene is ubiquitously expressed at all stages of Drosophila development and in Schneider cells. Similar to the human homologue, the ...

journal_title：Glycobiology

authors： Kim K,Lawrence SM,Park J,Pitts L,Vann WF,Betenbaugh MJ,Palter KB

更新日期：2002-02-01 00:00:00

abstract：:To investigate the developmental role of complex N-linked oligosaccharides, we previously inactivated the mouse Mgat1 gene which encodes UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI). Mgat1-null embryos developed morphogenic abnormalities by embryonic day (E) 9.5 a...

journal_title：Glycobiology

authors： Campbell RM,Metzler M,Granovsky M,Dennis JW,Marth JD

更新日期：1995-07-01 00:00:00

abstract：:A common argument against using plants as a production system for therapeutic proteins is their inability to perform authentic N-glycosylation. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex N-glycans as these nonmammalian N-glycan residues may provoke unwanted side ef...

journal_title：Glycobiology

authors： Jin C,Altmann F,Strasser R,Mach L,Schähs M,Kunert R,Rademacher T,Glössl J,Steinkellner H

更新日期：2008-03-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined. These cells clearly have processing glucosidases and mannosidases that can convert precursors to Man3GlcNAc2 structures...

journal_title：Glycobiology

authors： Jarvis DL,Bohlmeyer DA,Liao YF,Lomax KK,Merkle RK,Weinkauf C,Moremen KW

更新日期：1997-02-01 00:00:00

abstract：:We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...

journal_title：Glycobiology

authors： Kitova EN,Kitov PI,Bundle DR,Klassen JS

更新日期：2001-07-01 00:00:00

abstract：:We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demons...

journal_title：Glycobiology

authors： Kohda D,Yamada M,Igura M,Kamishikiryo J,Maenaka K

更新日期：2007-11-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...

journal_title：Glycobiology

authors： Chan AL,Morris HR,Panico M,Etienne AT,Rogers ME,Gaffney P,Creighton-Kempsford L,Dell A

更新日期：1991-03-01 00:00:00

abstract：:Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...

journal_title：Glycobiology

authors： Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WF

更新日期：2014-02-01 00:00:00

abstract：:Genetic polymorphism studies in humans provide unique opportunities to understand human biology and the mechanisms of diseases. Correlations between polymorphisms in the genes encoding human Siglecs and various diseases have been reported. Leading examples, such as the CD33 polymorphism associated with late-onset Alzh...

journal_title：Glycobiology

authors： Angata T

更新日期：2014-09-01 00:00:00

abstract：:Based on chemical analysis, we have previously concluded that the biologically important lipoarabinomannan (LAM) and lipomannan (LM) from Mycobacterium are multiglycosylated forms of the phosphatidylinositol mannosides (PIMs), the characteristic cell envelope mannophosphoinositides of mycobacteria. Using definitive an...

journal_title：Glycobiology

authors： Khoo KH,Dell A,Morris HR,Brennan PJ,Chatterjee D

更新日期：1995-02-01 00:00:00

abstract：:The Gram-negative bacterium Campylobacter jejuni 81116 (Penner serotype HS:6) has a class E lipooligosaccharide (LOS) biosynthesis locus containing 19 genes, which encode for 11 putative glycosyltransferases, 1 lipid A acyltransferase and 7 enzymes thought to be involved in the biosynthesis of dideoxyhexosamine (ddHex...

journal_title：Glycobiology

authors： Li ZZ,Riegert AS,Goneau MF,Cunningham AM,Vinogradov E,Li J,Schoenhofen IC,Thoden JB,Holden HM,Gilbert M

更新日期：2017-04-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...

journal_title：Glycobiology

authors： Li J,Rancour DM,Allende ML,Worth CA,Darling DS,Gilbert JB,Menon AK,Young WW Jr

更新日期：2001-03-01 00:00:00

abstract：:Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...

journal_title：Glycobiology

authors： Dhume ST,Stears RL,Lennarz WJ

更新日期：1996-01-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00

abstract：:Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...

journal_title：Glycobiology

authors： Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue S

更新日期：1999-09-01 00:00:00

abstract：:Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...

journal_title：Glycobiology

authors： Larsson O

更新日期：1993-10-01 00:00:00

abstract：:The Neu1 locus, in the S region of the murine histocompatibility-2 complex, regulates the sialic acid content of several liver lysosomal enzymes. Three alleles, Neu1a, Neu1b, and Neu1c, have been described on the basis of differential sialylation of the enzyme liver acid phosphatase. The Neu1a allele occurs in a small...

journal_title：Glycobiology

authors： Carrillo MB,Milner CM,Ball ST,Snoek M,Campbell RD

更新日期：1997-10-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00