Abstract:
:Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzyme that had been partially purified from medaka embryos (Seko,A., Kitajima,K., Inoue,Y. and Inoue,S. (1991) J. Biol. Chem., 266, 22110-22114). The apparent molecular weight of this enzyme was 150 K, and the optimal pH was 3.5-4.0, and the K m for L-hyosophorin was 44 microM. L-Hyosophorin is a cortical alveolus-derived glycononapeptide with a large N-linked glycan chain present in the perivitelline space of the developing embryo. Acid PNGase M was competitively inhibited by a free de-N-glycosylated nonapeptide derived from L-hyosophorin. This enzyme was expressed in ovaries and embryos at all developmental stages after gastrulation, but activity was not detected in embryos at developmental stages between fertilization and gastrula. Several independent lines of evidence suggested that acid PNGase M may be responsible for the unusual accumulation of free N-glycans derived from yolk glycoproteins (Iwasaki,M., Seko,A., Kitajima,K., Inoue,Y. and Inoue,S. (1992) J. Biol. Chem., 267, 24287-24296). In contrast, the neutral PNGase M was expressed in blastoderms from the 4-8 cell stage and in cells up to early gastrula. The general significance of these findings is that they show a developmental stage-dependent expression of the two PNGase activities, and that expression of the neutral PNGase M activity occurs concomitantly with the de-N-glycosylation of L-hyosophorin. These data thus support our conclusion that the neutral PNGase M is responsible for the developmental-stage-related de-N-glycosylation of the L-hyosophorin.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue Sdoi
10.1093/glycob/9.9.887subject
Has Abstractpub_date
1999-09-01 00:00:00pages
887-95issue
9eissn
0959-6658issn
1460-2423pii
cwc087journal_volume
9pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx067
更新日期:2017-10-01 00:00:00
abstract::The interaction of heparan sulfate (HS) (and the closely related molecule heparin) with FGF-1 is a requirement for enabling the growth factor to activate its cell surface tyrosine kinase receptor. However, little is known about the regulatory role of naturally occurring cell surface HS in FGF-1 activation. We have add...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.11.1183
更新日期:2000-11-01 00:00:00
abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.6.535
更新日期:1992-12-01 00:00:00
abstract::The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq028
更新日期:2010-06-01 00:00:00
abstract::We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp057
更新日期:2009-08-01 00:00:00
abstract::The human parasitic trematode Schistosoma mansoni has a complex life cycle that includes the freshwater snail Biomphalaria glabrata as intermediate host. Within each stage, the parasite synthesizes a wide array of glycoconjugates, exhibiting, in part, unique carbohydrate structures. In addition, the parasite expresses...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl048
更新日期:2007-01-01 00:00:00
abstract::Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi046
更新日期:2005-06-01 00:00:00
abstract::The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu009
更新日期:2014-05-01 00:00:00
abstract::A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg014
更新日期:2003-02-01 00:00:00
abstract::Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg041
更新日期:2003-06-01 00:00:00
abstract::Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy050
更新日期:2018-08-01 00:00:00
abstract::Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp084
更新日期:2009-09-01 00:00:00
abstract::Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.10.1037
更新日期:1999-10-01 00:00:00
abstract::Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.6.873
更新日期:1994-12-01 00:00:00
abstract::Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww093
更新日期:2017-03-15 00:00:00
abstract::Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr090
更新日期:2011-10-01 00:00:00
abstract::The evolutionarily conserved Notch signaling pathway plays broad and important roles during embryonic development and in adult tissue homeostasis. Unlike most other pathways used during animal development, Notch signaling does not rely on second messengers and intracellular signaling cascades. Instead, pathway activat...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq053
更新日期:2010-08-01 00:00:00
abstract::The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.1.7
更新日期:1998-01-01 00:00:00
abstract::In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr074
更新日期:2011-10-01 00:00:00
abstract::The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.1.15
更新日期:1993-02-01 00:00:00
abstract::In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt041
更新日期:2013-09-01 00:00:00
abstract::We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.3.217
更新日期:2001-03-01 00:00:00
abstract::We report the identification, molecular cloning, and characterization of an endo-beta-N-acetylglucosaminidase from the nematode Caenorhabditis elegans. A search of the C. elegans genome database revealed the existence of a gene exhibiting 34% identity to Mucor hiemalis (a fungus) endo-beta-N-acetylglucosaminidase (End...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf073
更新日期:2002-10-01 00:00:00
abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.8.813
更新日期:1995-12-01 00:00:00
abstract::Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integri...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/8.2.113
更新日期:1998-02-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00
abstract::Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1193
更新日期:1997-12-01 00:00:00
abstract::Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.5.475
更新日期:1993-10-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.6.777
更新日期:1997-09-01 00:00:00