Structural characterization of N-glycans from the freshwater snail Biomphalaria glabrata cross-reacting with Schistosoma mansoni glycoconjugates.

abstract：:Binding characteristics of two types of ligands for human neo-C-reactive protein (neo-CRP), which is a conformationally altered but physiologically relevant form of CRP, were studied fluorometrically by probing CRP immobilized on a polystyrene surface with europium-labeled ligands. Two Eu-ligands used were bovine seru...

journal_title：Glycobiology

authors： Lee RT,Lee YC

更新日期：2003-01-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:We expressed recombinant Cys subdomains in COS-7 cells to examine the role of this highly conserved protein domain in mucin biosynthesis. The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/...

journal_title：Glycobiology

authors： Perez-Vilar J,Randell SH,Boucher RC

更新日期：2004-04-01 00:00:00

abstract：:Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...

journal_title：Glycobiology

authors： Smith DF,Rangarajan D

更新日期：1995-03-01 00:00:00

abstract：:Lutrophin (LH) consists of an array of isoforms with different charges and bioactivities. This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges. Porcine LH (pLH) was separated into four isoforms by isoelectric focusing (IEF), followed by subu...

journal_title：Glycobiology

authors： Ujihara M,Yamamoto K,Nomura K,Toyoshima S,Demura H,Nakamura Y,Ohmura K,Osawa T

更新日期：1992-06-01 00:00:00

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...

journal_title：Glycobiology

authors： Vandersall-Nairn AS,Merkle RK,O'Brien K,Oeltmann TN,Moremen KW

更新日期：1998-12-01 00:00:00

abstract：:The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...

journal_title：Glycobiology

authors： Yannariello-Brown J,Zhou B,Weigel PH

更新日期：1997-02-01 00:00:00

abstract：:Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...

journal_title：Glycobiology

authors： Stray SJ,Cummings RD,Air GM

更新日期：2000-07-01 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

journal_title：Glycobiology

authors： Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

更新日期：1997-12-01 00:00:00

abstract：:Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...

journal_title：Glycobiology

authors： Clark SL,Santin AE,Bryant PA,Holman R,Rodnick KJ

更新日期：2013-11-01 00:00:00

abstract：:Isolation of glycopeptides utilizing hydrogen bonding between glycopeptide glycans and a carbohydrate-gel matrix in the organic phase is useful for site-specific characterization of oligosaccharides of glycoproteins, when combined with mass spectrometry. In this study, recovery of glycopeptides was improved by includi...

journal_title：Glycobiology

authors： Tajiri M,Yoshida S,Wada Y

更新日期：2005-12-01 00:00:00

abstract：:The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...

journal_title：Glycobiology

authors： Chen CP,Song SC,Gilboa-Garber N,Chang KS,Wu AM

更新日期：1998-01-01 00:00:00

abstract：:Angiogenesis is associated with growth, invasion, and metastasis of human solid tumors. Aberrant activation of endothelial cells and induction of microvascular permeability by a vascular endothelial growth factor (VEGF) receptor-2 (VEGFR-2) signaling pathway is observed in pathological angiogenesis including tumor, wo...

journal_title：Glycobiology

authors： Chung TW,Kim SJ,Choi HJ,Kim KJ,Kim MJ,Kim SH,Lee HJ,Ko JH,Lee YC,Suzuki A,Kim CH

更新日期：2009-03-01 00:00:00

abstract：:Cancer-associated glycan structures can be both tumor markers and engines of disease progression. The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan. Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological an...

journal_title：Glycobiology

authors： Venturi G,Gomes Ferreira I,Pucci M,Ferracin M,Malagolini N,Chiricolo M,Dall'Olio F

更新日期：2019-09-20 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...

journal_title：Glycobiology

authors： Kawabe K,Tateyama D,Toyoda H,Kawasaki N,Hashii N,Nakao H,Matsumoto S,Nonaka M,Matsumura H,Hirose Y,Morita A,Katayama M,Sakuma M,Kawasaki N,Furue MK,Kawasaki T

更新日期：2013-03-01 00:00:00

abstract：:We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...

journal_title：Glycobiology

authors： Toomre DK,Varki A

更新日期：1994-10-01 00:00:00

abstract：:Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...

journal_title：Glycobiology

authors： Amino R,Serrano AA,Morita OM,Pereira-Chioccola VL,Schenkman S

更新日期：1995-09-01 00:00:00

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...

journal_title：Glycobiology

authors： Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

更新日期：2017-10-01 00:00:00

abstract：:Since plants are emerging as an important system for the expression of recombinant glycoproteins, especially those intended for therapeutic purposes, it is important to scrutinize to what extent glycans harbored by mammalian glycoproteins produced in transgenic plants differ from their natural counterpart. We report h...

journal_title：Glycobiology

authors： Cabanes-Macheteau M,Fitchette-Lainé AC,Loutelier-Bourhis C,Lange C,Vine ND,Ma JK,Lerouge P,Faye L

更新日期：1999-04-01 00:00:00

abstract：:In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...

journal_title：Glycobiology

authors： Andersen DC,Goochee CF,Cooper G,Weitzhandler M

更新日期：1994-08-01 00:00:00

abstract：:The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.1...

journal_title：Glycobiology

authors： Ju T,Zheng Q,Cummings RD

更新日期：2006-10-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...

journal_title：Glycobiology

authors： Langsdorf A,Schumacher V,Shi X,Tran T,Zaia J,Jain S,Taglienti M,Kreidberg JA,Fine A,Ai X

更新日期：2011-02-01 00:00:00

abstract：:Influenza C virus spike glycoprotein HEF specifically recognizes glycoconjugates containing 9-O-acetyl-N-acetylneuraminic acid. The same protein also contains an esterase activity. Taking advantage of these two properties, influenza C virus was used as a very sensitive probe for the detection of traces of 9-O-acetyl-N...

journal_title：Glycobiology

authors： Zimmer G,Suguri T,Reuter G,Yu RK,Schauer R,Herrler G

更新日期：1994-06-01 00:00:00

abstract：:Heat stress on Madin-Darby canine kidney cells increased ceramide content to 187% at 40 degrees C for 24 h, and the de novo synthesis from serine increased to 146%. Glucosylceramide (GlcCer) and galactosylceramide (GalCer) synthesis from ceramide, the first glycosylation step of sphingolipid metabolism in kidney cells...

journal_title：Glycobiology

authors： Niimura Y,Moue T,Takahashi N,Nagai K

更新日期：2010-06-01 00:00:00

abstract：:Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is ...

journal_title：Glycobiology

authors： Yagi H,Yanagisawa M,Kato K,Yu RK

更新日期：2010-08-01 00:00:00

abstract：:Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined. These cells clearly have processing glucosidases and mannosidases that can convert precursors to Man3GlcNAc2 structures...

journal_title：Glycobiology

authors： Jarvis DL,Bohlmeyer DA,Liao YF,Lomax KK,Merkle RK,Weinkauf C,Moremen KW

更新日期：1997-02-01 00:00:00