Impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.


:Cancer-associated glycan structures can be both tumor markers and engines of disease progression. The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan. Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological and clinical significance remains unclear. To get insights into the clinical relevance of ST6GAL1 expression in CRC, we interrogated The Cancer Genome Atlas with mRNA expression data of hundreds of clinically characterized CRC and normal samples. We found an association of low ST6GAL1 expression with microsatellite instability (MSI), BRAF mutations and mucinous phenotype but not with stage, response to therapy and survival. To investigate the impact of ST6GAL1 expression in experimental systems, we analyzed the transcriptome and the phenotype of the CRC cell lines SW948 and SW48 after retroviral transduction with ST6GAL1 cDNA. The two cell lines display the two main pathways of CRC transformation: chromosomal instability and MSI, respectively. Constitutive ST6GAL1 expression induced much deeper transcriptomic changes in SW948 than in SW48 and affected different genes in the two cell lines. ST6GAL1 expression affected differentially the tyrosine phosphorylation induced by hepatocyte growth factor, the ability to grow in soft agar, to heal a scratch wound and to invade Matrigel in the two cell lines. These results indicate that the altered expression of a cancer-associated glycosyltransferase impacts the gene expression profile, as well as the phenotype, although in a cancer subtype-specific manner.






Venturi G,Gomes Ferreira I,Pucci M,Ferracin M,Malagolini N,Chiricolo M,Dall'Olio F




Has Abstract


2019-09-20 00:00:00














  • Carbo-loading in Coccidioides spp.: A quantitative analysis of CAZyme abundance and resulting glycan populations.

    abstract::Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingd...


    pub_type: 杂志文章


    authors: Mitchell NM,Grys TE,Lake DF

    更新日期:2020-02-19 00:00:00

  • Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha(1,3)-fucose and core xylose.

    abstract::The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...


    pub_type: 杂志文章


    authors: Bardor M,Faveeuw C,Fitchette AC,Gilbert D,Galas L,Trottein F,Faye L,Lerouge P

    更新日期:2003-06-01 00:00:00

  • Induction of sialyl-Lex expression by herpes simplex virus type 1 is dependent on viral immediate early RNA-activated transcription of host fucosyltransferase genes.

    abstract::We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra...


    pub_type: 杂志文章


    authors: Nyström K,Nordén R,Muylaert I,Elias P,Larson G,Olofsson S

    更新日期:2009-08-01 00:00:00

  • Recent advancements in understanding mammalian O-mannosylation.

    abstract::The post-translational glycosylation of select proteins by O-linked mannose (O-mannose or O-man) is a conserved modification from yeast to humans and has been shown to be necessary for proper development and growth. The most well studied O-mannosylated mammalian protein is α-dystroglycan (α-DG). Hypoglycosylation of α...


    pub_type: 杂志文章,评审


    authors: Sheikh MO,Halmo SM,Wells L

    更新日期:2017-09-01 00:00:00

  • Regulation of Mac-2BP secretion is mediated by its N-glycan binding to ERGIC-53.

    abstract::The leguminous-type (L-type) lectin ER-Golgi intermediate compartment (ERGIC)-53, a homo-oligomeric endoplasmic reticulum (ER)-Golgi recycling protein, functions as a transport receptor for newly synthesized glycoproteins in the early secretory pathway. Although a limited subset of cargo glycoproteins transported by E...


    pub_type: 杂志文章


    authors: Chen Y,Hojo S,Matsumoto N,Yamamoto K

    更新日期:2013-07-01 00:00:00

  • Novel thioglycoside analogs of α-galactosylceramide stimulate cytotoxicity and preferential Th1 cytokine production by human invariant natural killer T cells.

    abstract::Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...


    pub_type: 杂志文章


    authors: Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

    更新日期:2018-07-01 00:00:00

  • Rigorous analysis of free solution glycosaminoglycan dynamics using simple, new tools.

    abstract::Heparin/heparan sulfates (H/HS) are ubiquitous biopolymers that interact with many proteins to induce a range of biological functions. Unfortunately, how these biopolymers recognize their preferred protein targets remain poorly understood. It is suggested that computational simulations offer attractive avenues but a n...


    pub_type: 杂志文章


    authors: Nagarajan B,Sankaranarayanan NV,Desai UR

    更新日期:2020-07-16 00:00:00

  • Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.

    abstract::The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...


    pub_type: 杂志文章


    authors: Kajiura H,Seki T,Fujiyama K

    更新日期:2010-06-01 00:00:00

  • Lactose binding to human galectin-7 (p53-induced gene 1) induces long-range effects through the protein resulting in increased dimer stability and evidence for positive cooperativity.

    abstract::The product of p53-induced gene 1 is a member of the galectin family, i.e., galectin-7 (Gal-7). To move beyond structural data by X-ray diffraction, we initiated the study of the lectin by nuclear magnetic resonance (NMR) and circular dichroism spectroscopies, and molecular dynamics (MD) simulations. In concert, our r...


    pub_type: 杂志文章


    authors: Ermakova E,Miller MC,Nesmelova IV,López-Merino L,Berbís MA,Nesmelov Y,Tkachev YV,Lagartera L,Daragan VA,André S,Cañada FJ,Jiménez-Barbero J,Solís D,Gabius HJ,Mayo KH

    更新日期:2013-05-01 00:00:00

  • Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes.

    abstract::Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...


    pub_type: 杂志文章


    authors: Mishra S,Chatterjee S

    更新日期:2014-06-01 00:00:00

  • Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells.

    abstract::The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...


    pub_type: 杂志文章


    authors: Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

    更新日期:2008-12-01 00:00:00

  • The N-glycans of Trichomonas vaginalis contain variable core and antennal modifications.

    abstract::Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than b...


    pub_type: 杂志文章


    authors: Paschinger K,Hykollari A,Razzazi-Fazeli E,Greenwell P,Leitsch D,Walochnik J,Wilson IB

    更新日期:2012-02-01 00:00:00

  • Mac-2: a versatile galactose-binding protein of mammalian tissues.

    abstract::Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...


    pub_type: 杂志文章,评审


    authors: Hughes RC

    更新日期:1994-02-01 00:00:00

  • The effect of oral treatment with 6-O-butanoyl castanospermine (MDL 28,574) in the murine zosteriform model of HSV-1 infection.

    abstract::Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...


    pub_type: 杂志文章


    authors: Bridges CG,Ahmed SP,Kang MS,Nash RJ,Porter EA,Tyms AS

    更新日期:1995-03-01 00:00:00

  • SR proteins and galectins: what's in a name?

    abstract::Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...


    pub_type: 杂志文章,评审


    authors: Haudek KC,Patterson RJ,Wang JL

    更新日期:2010-10-01 00:00:00

  • Involvement of sialic acid in the regulation of γ--aminobutyric acid uptake activity of γ-aminobutyric acid transporter 1.

    abstract::The γ-aminobutyric acid (GABA) transporters (GATs) have long been recognized for their key role in the uptake of neurotransmitters. The GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins, which possess 12 putative transmembrane (TM) domains and three N-glycosylation sites on the extracellular lo...


    pub_type: 杂志文章


    authors: Hu J,Fei J,Reutter W,Fan H

    更新日期:2011-03-01 00:00:00

  • Minimum substrate requirements of endoglycosidase activities toward dermatan sulfate by electrospray ionization-tandem mass spectrometry.

    abstract::The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...


    pub_type: 杂志文章


    authors: Nielsen TC,Meikle PJ,Hopwood JJ,Fuller M

    更新日期:2008-12-01 00:00:00

  • Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: evidence for endogenous sialic acid biosynthetic ability in insects.

    abstract::In this study, we report the first cloning and characterization of a N-acetylneuraminic acid phosphate synthase gene from Drosophila melanogaster, an insect in the protostome lineage. The gene is ubiquitously expressed at all stages of Drosophila development and in Schneider cells. Similar to the human homologue, the ...


    pub_type: 杂志文章


    authors: Kim K,Lawrence SM,Park J,Pitts L,Vann WF,Betenbaugh MJ,Palter KB

    更新日期:2002-02-01 00:00:00

  • Inhibition of glycoprotein processing by L-fructose and L-xylulose.

    abstract::A number of unusual and rare carbohydrates were tested as potential inhibitors of various glycosidases, as well as inhibitors of N-linked oligosaccharide processing. The best inhibitors of several arylglycosidases and of glucosidase I were L-xylulose and L-fructose. Both of these sugars showed some inhibitory activity...


    pub_type: 杂志文章


    authors: Muniruzzaman S,Pan YT,Zeng Y,Atkins B,Izumori K,Elbein AD

    更新日期:1996-12-01 00:00:00

  • Concanavalin A binding to HIV envelope protein is less sensitive to mutations in glycosylation sites than monoclonal antibody 2G12.

    abstract::Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...


    pub_type: 杂志文章


    authors: Pashov A,MacLeod S,Saha R,Perry M,VanCott TC,Kieber-Emmons T

    更新日期:2005-10-01 00:00:00

  • The Giardia intestinalis filamentous cyst wall contains a novel beta(1-3)-N-acetyl-D-galactosamine polymer: a structural and conformational study.

    abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...


    pub_type: 杂志文章


    authors: Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

    更新日期:2002-08-01 00:00:00

  • Glycobiology of neuromuscular disorders.

    abstract::There has been a recent explosion in the identification of neuromuscular diseases caused by mutations in genes that affect carbohydrate metabolism or protein glycosylation. A number of these findings relate to defects in the glycosylation of alpha dystroglycan. Alpha dystroglycan is an essential component of the dystr...


    pub_type: 杂志文章,评审


    authors: Martin PT,Freeze HH

    更新日期:2003-08-01 00:00:00

  • MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas.

    abstract::Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...


    pub_type: 杂志文章


    authors: Conze T,Carvalho AS,Landegren U,Almeida R,Reis CA,David L,Söderberg O

    更新日期:2010-02-01 00:00:00

  • High-level expression of the Endo-beta-N-acetylglucosaminidase F2 gene in E.coli: one step purification to homogeneity.

    abstract::The Endo F2gene was overexpressed in E.coli as a fusion protein joined to the maltose-binding protein. MBP-Endo F2was found in a highly enriched state as insoluble, inactive inclusion bodies. Extraction of the inclusion bodies with 20% acetic acid followed by exhaustive dialysis rendered the fusion protein active and ...


    pub_type: 杂志文章


    authors: Reddy A,Grimwood BG,Plummer TH,Tarentino AL

    更新日期:1998-06-01 00:00:00

  • Urinary excretion of glycosaminoglycans and albumin in experimental diabetes mellitus.

    abstract::Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of ...


    pub_type: 杂志文章


    authors: Cadaval RA,Kohlman O,Michelacci YM

    更新日期:2000-02-01 00:00:00

  • The alpha-galactomannan Davanat binds galectin-1 at a site different from the conventional galectin carbohydrate binding domain.

    abstract::Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...


    pub_type: 杂志文章


    authors: Miller MC,Klyosov A,Mayo KH

    更新日期:2009-09-01 00:00:00

  • Induction of sialic acid 9-O-acetylation by diverse gene products: implications for the expression cloning of sialic acid O-acetyltransferases.

    abstract::Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...


    pub_type: 杂志文章


    authors: Shi WX,Chammas R,Varki A

    更新日期:1998-02-01 00:00:00

  • Chondroitin sulfate N-acetylgalactosaminyltransferase-1 (CSGalNAcT-1) involved in chondroitin sulfate initiation: Impact of sulfation on activity and specificity.

    abstract::Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...


    pub_type: 杂志文章


    authors: Gulberti S,Jacquinet JC,Chabel M,Ramalanjaona N,Magdalou J,Netter P,Coughtrie MW,Ouzzine M,Fournel-Gigleux S

    更新日期:2012-04-01 00:00:00

  • Utilization of major fucosylated and sialylated human milk oligosaccharides by isolated human gut microbes.

    abstract::Human milk oligosaccharides (HMOS) are not digested in the proximal intestine. In distal intestine, HMOS collectively modify the microbiota, but the response of individual bacteria to individual components of the HMOS is not well defined. Here, each of 25 major isolates of the human intestinal microbiota was fed indiv...


    pub_type: 杂志文章


    authors: Yu ZT,Chen C,Newburg DS

    更新日期:2013-11-01 00:00:00

  • Modulation of the expression of the rabbit galectin-3 gene by p53 and c-Ha-ras proteins and PMA.

    abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...


    pub_type: 杂志文章


    authors: Gaudin JC,Arar C,Monsigny M,Legrand A

    更新日期:1997-12-01 00:00:00