Abstract:
:Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Wu ZL,Person AD,Burton AJ,Singh R,Burroughs B,Fryxell D,Tatge TJ,Manning T,Wu G,Swift KAD,Kalabokis Vdoi
10.1093/glycob/cwz058subject
Has Abstractpub_date
2019-10-21 00:00:00pages
750-754issue
11eissn
0959-6658issn
1460-2423pii
5540431journal_volume
29pub_type
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