Abstract:
:L-Selectin is a lectin-like receptor on lymphocytes which mediates their attachment to high endothelial venules (HEV) within lymph nodes. Previous work has identified HEV-associated endothelial ligands for L-selectin as sialylated, fucosylated and sulphated glycoproteins of approximately 50 kDa and approximately 90 kDa (Sgp50 and Sgp90). The interaction of L-selectin with these ligands is carbohydrate directed, reflecting the involvement of its amino-terminal, calcium-type lectin domain. It has been reported, and we have confirmed, that anti-Ly22 blocks the adhesive function of L-selectin without reducing its binding to a carbohydrate- based ligand PPME (phosphomannan monoester core from Hansenula hostii). The epitope for this monoclonal antibody depends on the epidermal growth factor (EGF) domain of L-selectin. We demonstrate that anti-Ly22 inhibits the interaction of L-selectin with both of the Sgps, thus establishing that the interaction of L-selectin with HEV can be accounted for by the Sgps. Furthermore, the interaction of trypsin fragments of Sgp50 with L-selectin is inhibitable both by an antibody that maps to the lectin domain and by anti-Ly22. These findings raise the possibility that anti-Ly22 is affecting the function of the lectin domain of L-selectin rather than directly antagonizing the EGF domain. Toward a further characterization of L-selectin's carbohydrate specificity, we show that Sgp50 is partially inactivated by the linkage-specific Newcastle Disease virus sialidase (alpha 2,3 linkage). We additionally demonstrate that a sialyl Lewis x-related tetrasaccharide can interact with L-selectin, as has also been demonstrated for E-selectin and P-selectin.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Imai Y,Lasky LA,Rosen SDdoi
10.1093/glycob/2.4.373subject
Has Abstractpub_date
1992-08-01 00:00:00pages
373-81issue
4eissn
0959-6658issn
1460-2423journal_volume
2pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.7.705
更新日期:1999-07-01 00:00:00
abstract::It is widely reported that derivatives of sugar moieties can be used to metabolically label cell surface carbohydrates or inhibit a particular glycosylation. However, few studies address the effect of substitution of the cytidylmonophosphate (CMP) portion on sialyltransferase activities. Here we first synthesized 2'-O...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm132
更新日期:2008-02-01 00:00:00
abstract::Cell-surface glycans are functional docking sites for tissue lectins such as the members of the galectin family. This interaction triggers a wide variety of responses; hence, there is a keen interest in defining its structural features. Toward this aim, we have used enzyme-linked lectinosorbent (ELLSA) and inhibition ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj102
更新日期:2006-06-01 00:00:00
abstract::The glycobiology of the cestodes, a class of parasitic flatworms, is still largely unexplored. An important cestode species is Echinococcus granulosus, the tissue-dwelling larval stage of which causes hydatid disease. The E. granulosus larva is protected from the host by a massive mucin-based extracellular matrix term...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws220
更新日期:2013-04-01 00:00:00
abstract::The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.1.15
更新日期:1993-02-01 00:00:00
abstract::Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.8.791
更新日期:1998-08-01 00:00:00
abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf059
更新日期:2002-08-01 00:00:00
abstract::Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp077
更新日期:2009-12-01 00:00:00
abstract::Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt019
更新日期:2013-07-01 00:00:00
abstract::Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.4.579
更新日期:1997-06-01 00:00:00
abstract::The specificities of glycosaminoglycan (GAG) modification enzymes, particularly sulfotransferases, and the locations and concentrations of these enzymes in the Golgi apparatus give rise to the mature GAG polysaccharides that bind protein ligands. We studied the substrate specificities of sulfotransferases with a stabl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt033
更新日期:2013-08-01 00:00:00
abstract::Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.6.625
更新日期:1995-09-01 00:00:00
abstract::Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi083
更新日期:2005-10-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq133
更新日期:2011-02-01 00:00:00
abstract::In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx067
更新日期:2017-10-01 00:00:00
abstract::Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.3.323-e
更新日期:1997-04-01 00:00:00
abstract::Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glyca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz100
更新日期:2020-04-20 00:00:00
abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.5.395
更新日期:2001-05-01 00:00:00
abstract::The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn097
更新日期:2008-12-01 00:00:00
abstract::N-Glycanase 1, encoded by NGLY1, catalyzes the deglycosylation of misfolded N-linked glycoproteins retrotranslocated into the cytosol. We identified nine cases with mutations in NGLY1. The patients show developmental delay, seizures, peripheral neuropathy, abnormal liver function and alacrima (absence of tears). The m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv024
更新日期:2015-08-01 00:00:00
abstract::We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.3.217
更新日期:2001-03-01 00:00:00
abstract::O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu017
更新日期:2014-06-01 00:00:00
abstract::P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.7.695
更新日期:1998-07-01 00:00:00
abstract::Heparan sulfates (HSs) exert critical regulatory actions on many proteins, including growth factors, and are essential for normal development. Variations in their specific sulfation patterns are known to regulate binding and signaling of fibroblast growth factors (FGFs) via tyrosine kinase receptors (FGFRs). We previo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf072
更新日期:2002-11-01 00:00:00
abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz099
更新日期:2020-04-20 00:00:00
abstract::Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than b...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr149
更新日期:2012-02-01 00:00:00
abstract::Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq215
更新日期:2011-11-01 00:00:00
abstract::Cancer-derived heat shock protein gp96 induces a tumor-specific protective immune response primarily mediated by cytotoxic T lymphocytes (CTL) directed toward cancer-associated peptides associated with gp96. Both innate and adaptive immune responses have been demonstrated using a cell culture-based signaling mechanism...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp096
更新日期:2009-12-01 00:00:00
abstract::Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr052
更新日期:2011-09-01 00:00:00
abstract::N-Acetylglucosaminyltransferase-IV (GnT-IV) has two isoenzymes, GnT-IVa and GnT-IVb, which initiate the GlcNAcbeta1-4 branch synthesis on the Manalpha1-3 arm of the N-glycan core thereby increasing N-glycan branch complexity and conferring endogenous lectin binding epitopes. To elucidate the physiological significance...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp200
更新日期:2010-01-01 00:00:00