O-acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants.

abstract：:Shiga toxin (Stx) 2e of Stx-producing Escherichia coli (STEC) represents the major virulence factor responsible for the pig edema disease which is characterized by hemorrhagic lesions, neurological disorders and often fatal outcomes. Stx2e-producing strains from the intestine of slaughtered pigs (n = 3), feces of pigl...

journal_title：Glycobiology

authors： Müthing J,Meisen I,Zhang W,Bielaszewska M,Mormann M,Bauerfeind R,Schmidt MA,Friedrich AW,Karch H

更新日期：2012-06-01 00:00:00

abstract：:Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...

journal_title：Glycobiology

authors： Haudek KC,Patterson RJ,Wang JL

更新日期：2010-10-01 00:00:00

abstract：:Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...

journal_title：Glycobiology

authors： Fukuta K,Abe R,Yokomatsu T,Kono N,Asanagi M,Omae F,Minowa MT,Takeuchi M,Makino T

更新日期：2000-04-01 00:00:00

abstract：:Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...

journal_title：Glycobiology

authors： Kim MW,Rhee SK,Kim JY,Shimma Y,Chiba Y,Jigami Y,Kang HA

更新日期：2004-03-01 00:00:00

abstract：:Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...

journal_title：Glycobiology

authors： Kudoh A,Okawa Y,Shibata N

更新日期：2015-01-01 00:00:00

abstract：:The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...

journal_title：Glycobiology

authors： Yang S,Nikodem D,Davidson EA,Gowda DC

更新日期：1999-12-01 00:00:00

abstract：:Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...

journal_title：Glycobiology

authors： Pfeiffer G,Stirm S,Geyer R,Strube KH,Bergwerff AA,Kamerling JP,Vliegenthart JF

更新日期：1992-10-01 00:00:00

abstract：:The gut microbiota plays a major role in human health and an alteration in gut microbiota structure and function has been implicated in several diseases. In the colon, mucus covering the epithelium is critical to maintain a homeostatic relationship with the gut microbiota by harbouring a microbial community at safe di...

journal_title：Glycobiology

authors： Bell A,Juge N

更新日期：2020-10-09 00:00:00

abstract：:Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benef...

journal_title：Glycobiology

authors： Porfirio S,Archer-Hartmann S,Moreau GB,Ramakrishnan G,Haque R,Kirkpatrick BD,Petri WA,Azadi P

更新日期：2020-09-28 00:00:00

abstract：:The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...

journal_title：Glycobiology

authors： Kajiura H,Seki T,Fujiyama K

更新日期：2010-06-01 00:00:00

abstract：:A large family of UDP-GalNAc:polypeptide GalNAc transferases (ppGalNAc-Ts) initiates and defines sites of mucin-type Ser/Thr-O-GalNAc glycosylation. Family members have been classified into peptide- and glycopeptide-preferring subfamilies, although both families possess variable activities against glycopeptide substra...

journal_title：Glycobiology

authors： Revoredo L,Wang S,Bennett EP,Clausen H,Moremen KW,Jarvis DL,Ten Hagen KG,Tabak LA,Gerken TA

更新日期：2016-04-01 00:00:00

abstract：:The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...

journal_title：Glycobiology

authors： Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

更新日期：1999-07-01 00:00:00

abstract：:β-Glucans possess broad immunomodulatory properties, including activation of innate immune functions such as oxidative burst activity. The differential roles of complement receptor type 3 (CR3) and Dectin-1, the known β-glucan receptors, and their associated signaling pathways in the generation of oxidative burst indu...

journal_title：Glycobiology

authors： Bose N,Wurst LR,Chan AS,Dudney CM,LeRoux ML,Danielson ME,Will PM,Nodland SE,Patchen ML,Dalle Lucca JJ,Lebeda FJ,Vasilakos JP

更新日期：2014-04-01 00:00:00

abstract：:The deletion of the protein mannosyltransferase 1 gene (PMT1) of Saccharomyces cerevisiae results in viable cells. O-Mannosylation of proteins is reduced to about half of the value in comparison to wild-type cells. In order to distinguish between the the PMT1 gene product (= Pmt1p) and residual transferase activity, a...

journal_title：Glycobiology

authors： Gentzsch M,Strahl-Bolsinger S,Tanner W

更新日期：1995-02-01 00:00:00

abstract：:In the present study, we studied anti-Alzheimer's disease (AD) activities of chondroitin sulfate (CS) oligosaccharides with different molecular weights. CS from shark cartilage was degraded by a recombinant CS endolyase, chondroitinase ABC I (CHSase ABC I), and CS disaccharide (DP2), tetrasaccharide (DP4), hexasacchar...

journal_title：Glycobiology

authors： Zhao N,Meng J,Jiang W,Xu W,Liu C,Wang F

更新日期：2020-10-09 00:00:00

abstract：:We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...

journal_title：Glycobiology

authors： Itoh M,Fukumoto S,Baba N,Kuga Y,Mizuno A,Furukawa K

更新日期：1999-11-01 00:00:00

abstract：:The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...

journal_title：Glycobiology

authors： Masson E,Wiernsperger N,Lagarde M,El Bawab S

更新日期：2005-06-01 00:00:00

abstract：:The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes re...

journal_title：Glycobiology

authors： Ye L,Zheng X,Zheng H

更新日期：2014-04-01 00:00:00

abstract：:Human alpha-galactosidase A (alpha-Gal A) is the lysosomal glycohydrolase that cleaves the terminal alpha-galactosyl moieties of various glycoconjugates. Overexpression of the enzyme in Chinese hamster ovary (CHO) cells results in high intracellular enzyme accumulation and the selective secretion of active enzyme. Str...

journal_title：Glycobiology

authors： Matsuura F,Ohta M,Ioannou YA,Desnick RJ

更新日期：1998-04-01 00:00:00

abstract：:The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...

journal_title：Glycobiology

authors： Bardor M,Faveeuw C,Fitchette AC,Gilbert D,Galas L,Trottein F,Faye L,Lerouge P

更新日期：2003-06-01 00:00:00

abstract：:Glycosphingolipids are expressed on the cell membrane and act as important factors in various events that occur across the plasma membrane. Lactosylceramide (LacCer) is synthesized from glucosylceramide and is a common precursor of various glycosphingolipids existing in whole body. Based on the enzyme purification, β1...

journal_title：Glycobiology

authors： Tokuda N,Numata S,Li X,Nomura T,Takizawa M,Kondo Y,Yamashita Y,Hashimoto N,Kiyono T,Urano T,Furukawa K,Furukawa K

更新日期：2013-10-01 00:00:00

abstract：:CMP-beta-N-acetylneuraminic acid (CMP-neuNAc) is the substrate for the sialylation of glycoconjugates by sialyltransferases in microbes and higher eukaryotes. CMP-neuNAc synthetase catalyzes the formation of this substrate, CMP-neuNAc, from CTP and neuNAc. In this report we describe the purification of CMP-neuNAc synt...

journal_title：Glycobiology

authors： Vionnet J,Concepcion N,Warner T,Zapata G,Hanover J,Vann WF

更新日期：1999-05-01 00:00:00

abstract：:The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...

journal_title：Glycobiology

authors： Parker MJ,Gomery K,Richard G,MacKenzie CR,Cox AD,Richards JC,Evans SV

更新日期：2014-05-01 00:00:00

abstract：:Lymphocytic beta 1,4-galactosyltransferase (beta 1,4-GalTase, EC 2.4.1.38) activity was measured in B cells using a neoglyco-protein, N-acetylglucosamine-phenylisothiocyanate-bovine serum albumin (GlcNAc-pITC-BSA), as an acceptor substrate in a novel enzyme-linked immunosorbent assay (ELISA)-based method. This assay p...

journal_title：Glycobiology

authors： Keusch J,Lydyard PM,Isenberg DA,Delves PJ

更新日期：1995-06-01 00:00:00

abstract：:Beta-1,2-linked mannosides from Candida albicans phosphopeptidomannan (PPM) bind to macrophages through a receptor independent from the macrophage alpha-linked mannose receptor and stimulate these cells to secrete immune mediators. Anti-beta-1,2-linked mannoside but not anti-alpha-linked mannoside antibodies produced ...

journal_title：Glycobiology

authors： Jouault T,Fradin C,Dzierszinski F,Borg-Von-Zepelin M,Tomavo S,Corman R,Trinel PA,Kerckaert JP,Poulain D

更新日期：2001-08-01 00:00:00

abstract：:Apicomplexan protozoa are a phylum of parasites that includes pathogens such as Plasmodium, the causative agent of the most severe form of malaria responsible for almost 1 million deaths per year and Toxoplasma gondii causing toxoplasmosis, a disease leading to cerebral meningitis in immunocompromised individuals or t...

journal_title：Glycobiology

authors： Debierre-Grockiego F,Schwarz RT

更新日期：2010-07-01 00:00:00

abstract：:Algal fucoidan is an alpha-L-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the...

journal_title：Glycobiology

authors： Berteau O,McCort I,Goasdoué N,Tissot B,Daniel R

更新日期：2002-04-01 00:00:00

abstract：:Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...

journal_title：Glycobiology

authors： Miwa M,Horimoto T,Kiyohara M,Katayama T,Kitaoka M,Ashida H,Yamamoto K

更新日期：2010-11-01 00:00:00

abstract：:We have previously shown that chondroitin 6-sulfotransferase (C6ST) catalyzes transfer of sulfate not only to position 6 of GalNAc residue of chondroitin but also to position 6 of Gal residue of keratan sulfate. In this study, we examined the sulfation of sialyl lactosamine oligosaccharides by C6ST. C6ST catalyzed tra...

journal_title：Glycobiology

authors： Habuchi O,Suzuki Y,Fukuta M

更新日期：1997-04-01 00:00:00

abstract：:The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...

journal_title：Glycobiology

authors： Janoš P,Kozmon S,Tvaroška I,Koca J

更新日期：2016-07-01 00:00:00