Abstract:
:Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAc-alpha 2-6Gal beta 1-4GlcNAc beta 1-2[NeuAc alpha 2-3Gal beta 1- 4GlcNAc beta 1-4]-Man alpha 1-3[NeuAc alpha 2-3(SO4-6)Gal beta 1- 4-GlcNAc beta 1-2Man alpha 1-6]-Man beta 1-4GlcNAc beta 1- 4[Fuc alpha 1-6]GlcNAc-o1.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Pfeiffer G,Stirm S,Geyer R,Strube KH,Bergwerff AA,Kamerling JP,Vliegenthart JFdoi
10.1093/glycob/2.5.411subject
Has Abstractpub_date
1992-10-01 00:00:00pages
411-8issue
5eissn
0959-6658issn
1460-2423journal_volume
2pub_type
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