Sweating the small stuff: Glycoproteins in human sweat and their unexplored potential for microbial adhesion.

abstract：:Trehalose synthase (TreS) catalyzes the reversible conversion of maltose into trehalose in mycobacteria as one of three biosynthetic pathways to this nonreducing disaccharide. Given the importance of trehalose to survival of mycobacteria, there has been considerable interest in understanding the enzymes involved in it...

journal_title：Glycobiology

authors： Caner S,Nguyen N,Aguda A,Zhang R,Pan YT,Withers SG,Brayer GD

更新日期：2013-09-01 00:00:00

abstract：:Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...

journal_title：Glycobiology

authors： Miller MC,Klyosov A,Mayo KH

更新日期：2009-09-01 00:00:00

abstract：:Binding characteristics of two types of ligands for human neo-C-reactive protein (neo-CRP), which is a conformationally altered but physiologically relevant form of CRP, were studied fluorometrically by probing CRP immobilized on a polystyrene surface with europium-labeled ligands. Two Eu-ligands used were bovine seru...

journal_title：Glycobiology

authors： Lee RT,Lee YC

更新日期：2003-01-01 00:00:00

abstract：:The synthesis of the common and well-documented Siaalpha 2,6 to Galbeta 1,4GlcNAc structure (Sia6LacNAc) is principally mediated by the sialyltransferase ST6Gal I, which is particularly highly expressed in liver, lactating mammary gland, intestinal epithelia of newborn animals, and B cells. Multiple independent promot...

journal_title：Glycobiology

authors： Appenheimer MM,Huang RY,Chandrasekaran EV,Dalziel M,Hu YP,Soloway PD,Wuensch SA,Matta KL,Lau JT

更新日期：2003-08-01 00:00:00

abstract：:The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The pres...

journal_title：Glycobiology

authors： Thomsson KA,Prakobphol A,Leffler H,Reddy MS,Levine MJ,Fisher SJ,Hansson GC

更新日期：2002-01-01 00:00:00

abstract：:In addition to sialic acid, bacteria produce several other nonulosonic acids, including legionaminic acid (Leg). This has exactly the same stereochemistry as sialic acid, with the added features of 9-deoxy and 7-amino groups. In order to explore the biological effects of replacing sialic acid residues (Neu5Ac) in glyc...

journal_title：Glycobiology

authors： Watson DC,Leclerc S,Wakarchuk WW,Young NM

更新日期：2011-01-01 00:00:00

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:To facilitate deciphering the information content in the glycome, thin film-coated photoactivatable surfaces were applied for covalent immobilization of glycans, glycoconjugates, or lectins in microarray formats. Light-induced immobilization of a series of bacterial exopolysaccharides on photoactivatable dextran-coate...

journal_title：Glycobiology

authors： Angeloni S,Ridet JL,Kusy N,Gao H,Crevoisier F,Guinchard S,Kochhar S,Sigrist H,Sprenger N

更新日期：2005-01-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00

abstract：:The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for puta...

journal_title：Glycobiology

authors： Schallus T,Fehér K,Sternberg U,Rybin V,Muhle-Goll C

更新日期：2010-08-01 00:00:00

abstract：:There has been a recent explosion in the identification of neuromuscular diseases caused by mutations in genes that affect carbohydrate metabolism or protein glycosylation. A number of these findings relate to defects in the glycosylation of alpha dystroglycan. Alpha dystroglycan is an essential component of the dystr...

journal_title：Glycobiology

authors： Martin PT,Freeze HH

更新日期：2003-08-01 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...

journal_title：Glycobiology

authors： Kim JW,Budzak J,Liu Y,Jégouzo SAF,Drickamer K,Taylor ME

更新日期：2018-08-01 00:00:00

abstract：:The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...

journal_title：Glycobiology

authors： Fukushima K,Takasaki S

更新日期：1993-02-01 00:00:00

abstract：:trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...

journal_title：Glycobiology

authors： Cremona ML,Campetella O,Sánchez DO,Frasch AC

更新日期：1999-06-01 00:00:00

abstract：:Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...

journal_title：Glycobiology

authors： Peracaula R,Tabarés G,Royle L,Harvey DJ,Dwek RA,Rudd PM,de Llorens R

更新日期：2003-06-01 00:00:00

abstract：:Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respective...

journal_title：Glycobiology

authors： Weisshaar G,Hiyama J,Renwick AG

更新日期：1991-09-01 00:00:00

abstract：:We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...

journal_title：Glycobiology

authors： Kitova EN,Kitov PI,Bundle DR,Klassen JS

更新日期：2001-07-01 00:00:00

abstract：:The post-translational glycosylation of select proteins by O-linked mannose (O-mannose or O-man) is a conserved modification from yeast to humans and has been shown to be necessary for proper development and growth. The most well studied O-mannosylated mammalian protein is α-dystroglycan (α-DG). Hypoglycosylation of α...

journal_title：Glycobiology

authors： Sheikh MO,Halmo SM,Wells L

更新日期：2017-09-01 00:00:00

abstract：:Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. Here we describe the construction of a stable recom...

journal_title：Glycobiology

authors： Mattila P,Räbinä J,Hortling S,Helin J,Renkonen R

更新日期：2000-10-01 00:00:00

abstract：:Glycoside hydrolases are particularly abundant in all areas of metabolism as they are involved in the degradation of natural polysaccharides and glycoconjugates. These enzymes are classified into 133 families (CAZy server, http://www.cazy.org) in which members of each family have a similar structure and catalytic mech...

journal_title：Glycobiology

authors： André-Miral C,Koné FM,Solleux C,Grandjean C,Dion M,Tran V,Tellier C

更新日期：2015-04-01 00:00:00

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:The structure of the O-antigen polysaccharide (PS) from the Shiga-toxin producing Escherichia coli O63 has been elucidated using a combination of bioinformatics, component analyses and NMR spectroscopy. The O-antigen is comprised of tetrasaccharide repeating units with the following structure: →2)-β-d-Quip3N(d-allo-Th...

journal_title：Glycobiology

authors： Ståhle J,Fontana C,Weintraub A,Widmalm G

更新日期：2019-02-01 00:00:00

abstract：:O-antigen (O-polysaccharide), a part of the outer membrane of Gram-negative bacteria, is one of the most variable cell constituents and is related to bacterial virulence. O-antigen diversity is almost entirely due to genetic variations in O-antigen gene clusters. In this study, the O-polysaccharide structures of Salmo...

journal_title：Glycobiology

authors： Liu B,Perepelov AV,Svensson MV,Shevelev SD,Guo D,Senchenkova SN,Shashkov AS,Weintraub A,Feng L,Widmalm G,Knirel YA,Wang L

更新日期：2010-06-01 00:00:00

abstract：:Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...

journal_title：Glycobiology

authors： Tsuji S,Yamashita M,Hoffman DR,Nishiyama A,Shinohara T,Ohtsu T,Shibata Y

更新日期：2009-05-01 00:00:00

abstract：:The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.1...

journal_title：Glycobiology

authors： Ju T,Zheng Q,Cummings RD

更新日期：2006-10-01 00:00:00

abstract：:Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...

journal_title：Glycobiology

authors： Conze T,Carvalho AS,Landegren U,Almeida R,Reis CA,David L,Söderberg O

更新日期：2010-02-01 00:00:00

abstract：:Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benef...

journal_title：Glycobiology

authors： Porfirio S,Archer-Hartmann S,Moreau GB,Ramakrishnan G,Haque R,Kirkpatrick BD,Petri WA,Azadi P

更新日期：2020-09-28 00:00:00

abstract：:Lens culinaris lectin (LCA) is a useful probe for the detection in serum of a core-fucosylated alpha-fetoprotein, called AFP-L3 fraction, which is a well-known marker for the diagnosis and prognosis of hepatocellular carcinoma. Here we performed a systematic quantitative interaction analysis of LCA and its close homol...

journal_title：Glycobiology

authors： Tateno H,Nakamura-Tsuruta S,Hirabayashi J

更新日期：2009-05-01 00:00:00

abstract：:Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...

journal_title：Glycobiology

authors： Ganguly J,Low LY,Kamal N,Saile E,Forsberg LS,Gutierrez-Sanchez G,Hoffmaster AR,Liddington R,Quinn CP,Carlson RW,Kannenberg EL

更新日期：2013-07-01 00:00:00