The secondary cell wall polysaccharide of Bacillus anthracis provides the specific binding ligand for the C-terminal cell wall-binding domain of two phage endolysins, PlyL and PlyG.


:Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage lysin L (PlyL) and phage lysin G (PlyG), are specific for Bacillus anthracis. To date, the cell wall ligands for their C-terminal CBD have not been identified. We recently described structures for a number of secondary cell wall polysaccharides (SCWPs) from B. anthracis and B. cereus strains. They are covalently bound to the PG and are comprised of a -ManNAc-GlcNAc-HexNAc- backbone with various galactosyl or glucosyl substitutions. Surface plasmon resonance (SPR) showed that the endolysins PlyL and PlyG bind to the SCWP from B. anthracis (SCWPBa) with high affinity (i.e. in the μM range with dissociation constants ranging from 0.81 × 10(-6) to 7.51 × 10(-6) M). In addition, the PlyL and PlyG SCWPBa binding sites reside with their C-terminal domains. The dissociation constants for the interactions of these endolysins and their derived C-terminal domains with the SCWPBa were in the range reported for other protein-carbohydrate interactions. Our findings show that the SCWPBa is the ligand that confers PlyL and PlyG lysin binding and localization to the PG. PlyL and PlyG also bound the SCWP from B. cereus G9241 with comparable affinities to SCWPBa. No detectable binding was found to the SCWPs from B. cereus ATCC (American Type Culture Collection) 10987 and ATCC 14579, thus demonstrating specificity of lysin binding to SCWPs.






Ganguly J,Low LY,Kamal N,Saile E,Forsberg LS,Gutierrez-Sanchez G,Hoffmaster AR,Liddington R,Quinn CP,Carlson RW,Kannenberg EL




Has Abstract


2013-07-01 00:00:00














  • Characterization of a putative 3-deoxy-D-manno-2-octulosonic acid (Kdo) transferase gene from Arabidopsis thaliana.

    abstract::The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pa...


    pub_type: 杂志文章


    authors: Séveno M,Séveno-Carpentier E,Voxeur A,Menu-Bouaouiche L,Rihouey C,Delmas F,Chevalier C,Driouich A,Lerouge P

    更新日期:2010-05-01 00:00:00

  • Mouse anti-ceramide antiserum: a specific tool for the detection of endogenous ceramide.

    abstract::Ceramide is a pivotal molecule in signal transduction and an essential structural component of the epidermal permeability barrier. The epidermis is marked by a high concentration of ceramide and by a unique spectrum of ceramide species: Besides the two ceramide structures commonly found in mammalian tissue, N-acylsphi...


    pub_type: 杂志文章


    authors: Vielhaber G,Brade L,Lindner B,Pfeiffer S,Wepf R,Hintze U,Wittern KP,Brade H

    更新日期:2001-06-01 00:00:00

  • Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

    abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...


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    authors: Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

    更新日期:2016-07-01 00:00:00

  • Glycosylation and proteolytic processing of 70 kDa C-terminal recombinant polypeptides of Plasmodium falciparum merozoite surface protein 1 expressed in mammalian cells.

    abstract::The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...


    pub_type: 杂志文章


    authors: Yang S,Nikodem D,Davidson EA,Gowda DC

    更新日期:1999-12-01 00:00:00

  • A new beta-1,2-N-acetylglucosaminyltransferase that may play a role in the biosynthesis of mammalian O-mannosyl glycans.

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    pub_type: 杂志文章


    authors: Takahashi S,Sasaki T,Manya H,Chiba Y,Yoshida A,Mizuno M,Ishida H,Ito F,Inazu T,Kotani N,Takasaki S,Takeuchi M,Endo T

    更新日期:2001-01-01 00:00:00

  • Epidermal growth factor receptor glycosylation is required for ganglioside GM3 binding and GM3-mediated suppression [correction of suppresion] of activation.

    abstract::Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...


    pub_type: 杂志文章


    authors: Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

    更新日期:2001-07-01 00:00:00

  • Exploring the glycan interaction in vivo: Future prospects of neo-glycoproteins for diagnostics.

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    pub_type: 杂志文章,评审


    authors: Ogura A,Kurbangalieva A,Tanaka K

    更新日期:2016-08-01 00:00:00

  • Study on the relationships between molecular weights of chondroitin sulfate oligosaccharides and Aβ-induced oxidative stress and the related mechanisms.

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    pub_type: 杂志文章


    authors: Zhao N,Meng J,Jiang W,Xu W,Liu C,Wang F

    更新日期:2020-10-09 00:00:00

  • The role of MRH domain-containing lectins in ERAD.

    abstract::The endoplasmic reticulum (ER) quality control system ensures that newly synthesized proteins in the early secretory pathway are in the correct conformation. Polypeptides that have failed to fold into native conformers are subsequently retrotranslocated and degraded by the cytosolic ubiquitin-proteasome system, a proc...


    pub_type: 杂志文章,评审


    authors: Hosokawa N,Kamiya Y,Kato K

    更新日期:2010-06-01 00:00:00

  • Galectin-1 stimulates monocyte chemotaxis via the p44/42 MAP kinase pathway and a pertussis toxin-sensitive pathway.

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    authors: Malik RK,Ghurye RR,Lawrence-Watt DJ,Stewart HJ

    更新日期:2009-12-01 00:00:00

  • A new method for the analysis of amide-linked hydroxy fatty acids in lipid-As from gram-negative bacteria.

    abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...


    pub_type: 杂志文章


    authors: Bhat UR,Carlson RW

    更新日期:1992-12-01 00:00:00

  • Evolutionary considerations in relating oligosaccharide diversity to biological function.

    abstract::The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...


    pub_type: 杂志文章,评审


    authors: Gagneux P,Varki A

    更新日期:1999-08-01 00:00:00

  • Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice.

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    pub_type: 杂志文章


    authors: Takamatsu S,Antonopoulos A,Ohtsubo K,Ditto D,Chiba Y,Le DT,Morris HR,Haslam SM,Dell A,Marth JD,Taniguchi N

    更新日期:2010-01-01 00:00:00

  • Modification of sialic acids by 9-O-acetylation is detected in human leucocytes using the lectin property of influenza C virus.

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    pub_type: 杂志文章


    authors: Zimmer G,Suguri T,Reuter G,Yu RK,Schauer R,Herrler G

    更新日期:1994-06-01 00:00:00

  • An updated view of the oligosaccharyltransferase complex in Plasmodium.

    abstract::Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currentl...


    pub_type: 杂志文章


    authors: Tamana S,Promponas VJ

    更新日期:2019-05-01 00:00:00

  • Mouse C127 cells transfected with fucosyltransferase fuc-TIII express masked Lewisx but not Lewisx antigen.

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    pub_type: 杂志文章


    authors: Valli M,Bardoni A,Trinchera M

    更新日期:1999-01-01 00:00:00

  • Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: evidence for endogenous sialic acid biosynthetic ability in insects.

    abstract::In this study, we report the first cloning and characterization of a N-acetylneuraminic acid phosphate synthase gene from Drosophila melanogaster, an insect in the protostome lineage. The gene is ubiquitously expressed at all stages of Drosophila development and in Schneider cells. Similar to the human homologue, the ...


    pub_type: 杂志文章


    authors: Kim K,Lawrence SM,Park J,Pitts L,Vann WF,Betenbaugh MJ,Palter KB

    更新日期:2002-02-01 00:00:00

  • Rigorous analysis of free solution glycosaminoglycan dynamics using simple, new tools.

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    pub_type: 杂志文章


    authors: Nagarajan B,Sankaranarayanan NV,Desai UR

    更新日期:2020-07-16 00:00:00

  • Comparative analysis of core-fucose-binding lectins from Lens culinaris and Pisum sativum using frontal affinity chromatography.

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    pub_type: 杂志文章


    authors: Tateno H,Nakamura-Tsuruta S,Hirabayashi J

    更新日期:2009-05-01 00:00:00

  • Immunological reactions in response to apicomplexan glycosylphosphatidylinositols.

    abstract::Apicomplexan protozoa are a phylum of parasites that includes pathogens such as Plasmodium, the causative agent of the most severe form of malaria responsible for almost 1 million deaths per year and Toxoplasma gondii causing toxoplasmosis, a disease leading to cerebral meningitis in immunocompromised individuals or t...


    pub_type: 杂志文章,评审


    authors: Debierre-Grockiego F,Schwarz RT

    更新日期:2010-07-01 00:00:00

  • Strict specificity for high-mannose type N-glycans and primary structure of a red alga Eucheuma serra lectin.

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    pub_type: 杂志文章


    authors: Hori K,Sato Y,Ito K,Fujiwara Y,Iwamoto Y,Makino H,Kawakubo A

    更新日期:2007-05-01 00:00:00

  • Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors.

    abstract::The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...


    pub_type: 杂志文章


    authors: Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

    更新日期:2007-04-01 00:00:00

  • Full structural characterization of Shigella flexneri M90T serotype 5 wild-type R-LPS and its delta galU mutant: glycine residue location in the inner core of the lipopolysaccharide.

    abstract::Shigella flexneri is a gram-negative bacterium responsible for serious enteric infections that occur mainly in the terminal ileum and colon. High interest in Shigella, as a human pathogen, is driven by its antibiotic resistance and the necessity to develop a vaccine against its infections. Vaccines of the last generat...


    pub_type: 杂志文章


    authors: Molinaro A,Silipo A,Castro CD,Sturiale L,Nigro G,Garozzo D,Bernardini ML,Lanzetta R,Parrilli M

    更新日期:2008-03-01 00:00:00

  • Sea urchin egg receptor for sperm: the oligosaccharide chains stabilize sperm binding.

    abstract::Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...


    pub_type: 杂志文章


    authors: Dhume ST,Stears RL,Lennarz WJ

    更新日期:1996-01-01 00:00:00

  • Monitoring of immunoglobulin N- and O-glycosylation in health and disease.

    abstract::Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...


    pub_type: 杂志文章,评审


    authors: de Haan N,Falck D,Wuhrer M

    更新日期:2020-03-20 00:00:00

  • Sulfation of sialyl lactosamine oligosaccharides by chondroitin 6-sulfotransferase.

    abstract::We have previously shown that chondroitin 6-sulfotransferase (C6ST) catalyzes transfer of sulfate not only to position 6 of GalNAc residue of chondroitin but also to position 6 of Gal residue of keratan sulfate. In this study, we examined the sulfation of sialyl lactosamine oligosaccharides by C6ST. C6ST catalyzed tra...


    pub_type: 杂志文章


    authors: Habuchi O,Suzuki Y,Fukuta M

    更新日期:1997-04-01 00:00:00

  • A refined palate: bacterial consumption of host glycans in the gut.

    abstract::The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...


    pub_type: 杂志文章,评审


    authors: Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

    更新日期:2013-09-01 00:00:00

  • The observation of multivalent complexes of Shiga-like toxin with globotriaoside and the determination of their stoichiometry by nanoelectrospray Fourier-transform ion cyclotron resonance mass spectrometry.

    abstract::We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...


    pub_type: 杂志文章


    authors: Kitova EN,Kitov PI,Bundle DR,Klassen JS

    更新日期:2001-07-01 00:00:00

  • Expression regulation and function of heparan sulfate 6-O-endosulfatases in the spermatogonial stem cell niche.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...


    pub_type: 杂志文章


    authors: Langsdorf A,Schumacher V,Shi X,Tran T,Zaia J,Jain S,Taglienti M,Kreidberg JA,Fine A,Ai X

    更新日期:2011-02-01 00:00:00

  • Tn and STn are members of a family of carbohydrate tumor antigens that possess carbohydrate-carbohydrate interactions.

    abstract::The mucin-type O-glycome in cancer aberrantly expresses the truncated glycans Tn (GalNAcα1-Ser/Thr) and STn (Neu5Acα2,6GalNAcα1-Ser/Thr). However, the role of Tn and STn in cancer and other diseases is not well understood. Our recent discovery of the self-binding properties (carbohydrate-carbohydrate interactions, CCI...


    pub_type: 杂志文章,评审


    authors: Sletmoen M,Gerken TA,Stokke BT,Burchell J,Brewer CF

    更新日期:2018-07-01 00:00:00