The secondary cell wall polysaccharide of Bacillus anthracis provides the specific binding ligand for the C-terminal cell wall-binding domain of two phage endolysins, PlyL and PlyG.


:Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage lysin L (PlyL) and phage lysin G (PlyG), are specific for Bacillus anthracis. To date, the cell wall ligands for their C-terminal CBD have not been identified. We recently described structures for a number of secondary cell wall polysaccharides (SCWPs) from B. anthracis and B. cereus strains. They are covalently bound to the PG and are comprised of a -ManNAc-GlcNAc-HexNAc- backbone with various galactosyl or glucosyl substitutions. Surface plasmon resonance (SPR) showed that the endolysins PlyL and PlyG bind to the SCWP from B. anthracis (SCWPBa) with high affinity (i.e. in the μM range with dissociation constants ranging from 0.81 × 10(-6) to 7.51 × 10(-6) M). In addition, the PlyL and PlyG SCWPBa binding sites reside with their C-terminal domains. The dissociation constants for the interactions of these endolysins and their derived C-terminal domains with the SCWPBa were in the range reported for other protein-carbohydrate interactions. Our findings show that the SCWPBa is the ligand that confers PlyL and PlyG lysin binding and localization to the PG. PlyL and PlyG also bound the SCWP from B. cereus G9241 with comparable affinities to SCWPBa. No detectable binding was found to the SCWPs from B. cereus ATCC (American Type Culture Collection) 10987 and ATCC 14579, thus demonstrating specificity of lysin binding to SCWPs.






Ganguly J,Low LY,Kamal N,Saile E,Forsberg LS,Gutierrez-Sanchez G,Hoffmaster AR,Liddington R,Quinn CP,Carlson RW,Kannenberg EL




Has Abstract


2013-07-01 00:00:00














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    authors: Nordén R,Nyström K,Aurelius J,Brisslert M,Olofsson S

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    authors: Peracaula R,Tabarés G,Royle L,Harvey DJ,Dwek RA,Rudd PM,de Llorens R

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    authors: Liu B,Perepelov AV,Svensson MV,Shevelev SD,Guo D,Senchenkova SN,Shashkov AS,Weintraub A,Feng L,Widmalm G,Knirel YA,Wang L

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    pub_type: 杂志文章


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    更新日期:2016-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Lin G,Todeschini AR,Koizumi A,Neves JL,González H,Dematteis S,Hada N,Previato JO,Ferreira F,Mendonça-Previato L,Díaz A

    更新日期:2013-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Mast SW,Diekman K,Karaveg K,Davis A,Sifers RN,Moremen KW

    更新日期:2005-04-01 00:00:00

  • Mutation of Asn128 to Asp of Phaseolus vulgaris leucoagglutinin (PHA-L) eliminates carbohydrate-binding and biological activity.

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    pub_type: 杂志文章


    authors: Mirkov TE,Chrispeels MJ

    更新日期:1993-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Appenheimer MM,Huang RY,Chandrasekaran EV,Dalziel M,Hu YP,Soloway PD,Wuensch SA,Matta KL,Lau JT

    更新日期:2003-08-01 00:00:00

  • Glucocorticoids and microbiota regulate ontogeny of intestinal fucosyltransferase 2 requisite for gut homeostasis.

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    pub_type: 杂志文章


    authors: Nanthakumar NN,Meng D,Newburg DS

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    authors: Yang S,Nikodem D,Davidson EA,Gowda DC

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    authors: Alverez K,Haswell C,St Clair M,Perng GS,Shorebah M,Pierce M,Fregien N

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    authors: Iwamoto S,Isoyama M,Hirano M,Yamaya K,Ito Y,Matsuo I,Totani K

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    pub_type: 杂志文章,评审


    authors: Weigel PH

    更新日期:2017-09-01 00:00:00

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    authors: Sheikh MO,Halmo SM,Wells L

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    authors: Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten U

    更新日期:1997-10-01 00:00:00

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    pub_type: 杂志文章,评审


    authors: Camby I,Le Mercier M,Lefranc F,Kiss R

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  • Crystal structures of a β-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulfide bond-mediated dimeric forms.

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    pub_type: 杂志文章


    authors: Khan F,Kurre D,Suguna K

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  • Characterization of the meningococcal serogroup X capsule N-acetylglucosamine-1-phosphotransferase.

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    authors: Ju T,Zheng Q,Cummings RD

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    pub_type: 杂志文章


    authors: Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

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    pub_type: 杂志文章


    authors: Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

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    pub_type: 杂志文章


    authors: Niimura Y,Moue T,Takahashi N,Nagai K

    更新日期:2010-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Takamatsu S,Antonopoulos A,Ohtsubo K,Ditto D,Chiba Y,Le DT,Morris HR,Haslam SM,Dell A,Marth JD,Taniguchi N

    更新日期:2010-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

    更新日期:2019-11-20 00:00:00

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    pub_type: 杂志文章


    authors: Li LX,Ashikov A,Liu H,Griffith CL,Bakker H,Doering TL

    更新日期:2017-01-01 00:00:00

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    abstract::Galectin-8 (gal-8) is a "tandem-repeat"-type galectin, containing two carbohydrate recognition domains connected by a linker peptide. gal-8 is expressed both in the cytoplasm and nucleus in vascular endothelial cells (ECs) from normal and tumor-associated blood vessels, and in lymphatic endothelial cells. Herein, we d...


    pub_type: 杂志文章,评审


    authors: Troncoso MF,Ferragut F,Bacigalupo ML,Cárdenas Delgado VM,Nugnes LG,Gentilini L,Laderach D,Wolfenstein-Todel C,Compagno D,Rabinovich GA,Elola MT

    更新日期:2014-10-01 00:00:00