The biosynthesis of Lewis X in Helicobacter pylori.

abstract：:Apiose is a unique branched-chain pentose found principally in plants. It is a key component of structurally complex cell wall polysaccharides, as well as being present in a large number of naturally occurring secondary metabolites. This review provides a comprehensive overview of the current state of knowledge on the...

journal_title：Glycobiology

authors： Pičmanová M,Møller BL

更新日期：2016-05-01 00:00:00

abstract：:N-Acetylglucosaminlyltransferase-V (GnT-V) synthesizes GlcNAcbeta1,6Man branched N-glycans both in vitro and in vivo. A paralog, GnT-Vb (or GnT-IX), has also been shown to synthesize both GlcNAcbeta1,6Man branched N- and O-glycans. GnT-V is expressed in most human and rodent tissues while GnT-Vb expression is limited ...

journal_title：Glycobiology

authors： Alvarez-Manilla G,Troupe K,Fleming M,Martinez-Uribe E,Pierce M

更新日期：2010-02-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00

abstract：:Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...

journal_title：Glycobiology

authors： Kim JW,Budzak J,Liu Y,Jégouzo SAF,Drickamer K,Taylor ME

更新日期：2018-08-01 00:00:00

abstract：:Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...

journal_title：Glycobiology

authors： Weigel PH

更新日期：2017-09-01 00:00:00

abstract：:Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...

journal_title：Glycobiology

authors： Dairaku K,Spiro RG

更新日期：1997-06-01 00:00:00

abstract：:Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currentl...

journal_title：Glycobiology

authors： Tamana S,Promponas VJ

更新日期：2019-05-01 00:00:00

abstract：:The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pa...

journal_title：Glycobiology

authors： Séveno M,Séveno-Carpentier E,Voxeur A,Menu-Bouaouiche L,Rihouey C,Delmas F,Chevalier C,Driouich A,Lerouge P

更新日期：2010-05-01 00:00:00

abstract：:Genetic polymorphism studies in humans provide unique opportunities to understand human biology and the mechanisms of diseases. Correlations between polymorphisms in the genes encoding human Siglecs and various diseases have been reported. Leading examples, such as the CD33 polymorphism associated with late-onset Alzh...

journal_title：Glycobiology

authors： Angata T

更新日期：2014-09-01 00:00:00

abstract：:The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid tre...

journal_title：Glycobiology

authors： Lawrence CW,Little PA,Little BW,Glushka J,van Halbeek H,Alhadeff JA

更新日期：1993-06-01 00:00:00

abstract：:Protein O-fucosyltransferase 1 (Pofut1) and protein O-fucosyltransferase 2 (Pofut2) add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively. Glycan chain elongation past O-fucose can occur to yield a tetrasa...

journal_title：Glycobiology

authors： Al-Shareffi E,Chaubard JL,Leonhard-Melief C,Wang SK,Wong CH,Haltiwanger RS

更新日期：2013-02-01 00:00:00

abstract：:The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...

journal_title：Glycobiology

authors： Parker W,Lateef J,Everett ML,Platt JL

更新日期：1996-07-01 00:00:00

abstract：:Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...

journal_title：Glycobiology

authors： Miwa M,Horimoto T,Kiyohara M,Katayama T,Kitaoka M,Ashida H,Yamamoto K

更新日期：2010-11-01 00:00:00

abstract：:Affinities of the human blood group glycosyltransferases, alpha-(1-->3)-N-acetylgalactosaminyltransferase (GTA) and alpha-(1-->3)-galactosyltransferase (GTB) for their common acceptor substrate alpha-l-Fucp-(1-->2)-beta-d-Galp-O(CH2)(7)CH3 (1), in the absence and presence of bound uridine 5'-diphosphate (UDP) and Mn2+...

journal_title：Glycobiology

authors： Shoemaker GK,Soya N,Palcic MM,Klassen JS

更新日期：2008-08-01 00:00:00

abstract：:The leguminous-type (L-type) lectin ER-Golgi intermediate compartment (ERGIC)-53, a homo-oligomeric endoplasmic reticulum (ER)-Golgi recycling protein, functions as a transport receptor for newly synthesized glycoproteins in the early secretory pathway. Although a limited subset of cargo glycoproteins transported by E...

journal_title：Glycobiology

authors： Chen Y,Hojo S,Matsumoto N,Yamamoto K

更新日期：2013-07-01 00:00:00

abstract：:Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...

journal_title：Glycobiology

authors： Kaida K,Ariga T,Yu RK

更新日期：2009-07-01 00:00:00

abstract：:Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...

journal_title：Glycobiology

authors： Ikarashi K,Fujiwara H,Yamazaki Y,Goto J,Kaneko K,Kato H,Fujii S,Sasaki H,Fukumoto S,Furukawa K,Waki H,Furukawa K

更新日期：2011-10-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:Glycosylation determines essential biological functions of epithelial mucins in health and disease. We report on the influence of glycosylation of the immunodominant DTR motif of MUC1 on its antigenicity. Sets of novel glycopeptides were synthesized that enabled us to examine sole and combined effects of peptide lengt...

journal_title：Glycobiology

authors： Karsten U,Serttas N,Paulsen H,Danielczyk A,Goletz S

更新日期：2004-08-01 00:00:00

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00

abstract：:Shiga toxin (Stx) 2e of Stx-producing Escherichia coli (STEC) represents the major virulence factor responsible for the pig edema disease which is characterized by hemorrhagic lesions, neurological disorders and often fatal outcomes. Stx2e-producing strains from the intestine of slaughtered pigs (n = 3), feces of pigl...

journal_title：Glycobiology

authors： Müthing J,Meisen I,Zhang W,Bielaszewska M,Mormann M,Bauerfeind R,Schmidt MA,Friedrich AW,Karch H

更新日期：2012-06-01 00:00:00

abstract：:Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

journal_title：Glycobiology

authors： Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

更新日期：2006-06-01 00:00:00

abstract：:We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...

journal_title：Glycobiology

authors： Li J,Rancour DM,Allende ML,Worth CA,Darling DS,Gilbert JB,Menon AK,Young WW Jr

更新日期：2001-03-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:Core alpha1,6-fucosylation is a conserved feature of animal N-linked oligosaccharides being present in both invertebrates and vertebrates. To prove that the enzymatic basis for this modification is also evolutionarily conserved, cDNAs encoding the catalytic regions of the predicted Caenorhabditis elegans and Drosophil...

journal_title：Glycobiology

authors： Paschinger K,Staudacher E,Stemmer U,Fabini G,Wilson IB

更新日期：2005-05-01 00:00:00

abstract：:Lymphocytic beta 1,4-galactosyltransferase (beta 1,4-GalTase, EC 2.4.1.38) activity was measured in B cells using a neoglyco-protein, N-acetylglucosamine-phenylisothiocyanate-bovine serum albumin (GlcNAc-pITC-BSA), as an acceptor substrate in a novel enzyme-linked immunosorbent assay (ELISA)-based method. This assay p...

journal_title：Glycobiology

authors： Keusch J,Lydyard PM,Isenberg DA,Delves PJ

更新日期：1995-06-01 00:00:00

abstract：:A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...

journal_title：Glycobiology

authors： Abe H,Shimma Y,Jigami Y

更新日期：2003-02-01 00:00:00

abstract：:We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...

journal_title：Glycobiology

authors： Shoji H,Nishi N,Hirashima M,Nakamura T

更新日期：2002-03-01 00:00:00

abstract：:Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...

journal_title：Glycobiology

authors： Wojczyk BS,Takahashi N,Levy MT,Andrews DW,Abrams WR,Wunner WH,Spitalnik SL

更新日期：2005-06-01 00:00:00