Recent advancements in understanding mammalian O-mannosylation.

abstract：:Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...

journal_title：Glycobiology

authors： Shi WX,Chammas R,Varki A

更新日期：1998-02-01 00:00:00

abstract：:Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...

journal_title：Glycobiology

authors： Miller MC,Klyosov A,Mayo KH

更新日期：2009-09-01 00:00:00

abstract：:Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. Galectin-1 (Gal-1), a prototype member of the galectin family, displays only one ca...

journal_title：Glycobiology

authors： Romero JM,Trujillo M,Estrin DA,Rabinovich GA,Di Lella S

更新日期：2016-12-01 00:00:00

abstract：:The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...

journal_title：Glycobiology

authors： Parker MJ,Gomery K,Richard G,MacKenzie CR,Cox AD,Richards JC,Evans SV

更新日期：2014-05-01 00:00:00

abstract：:Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...

journal_title：Glycobiology

authors： Ziegler FD,Trimble RB

更新日期：1991-12-01 00:00:00

abstract：:The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...

journal_title：Glycobiology

authors： Parker W,Lateef J,Everett ML,Platt JL

更新日期：1996-07-01 00:00:00

abstract：:Glycoside hydrolases are particularly abundant in all areas of metabolism as they are involved in the degradation of natural polysaccharides and glycoconjugates. These enzymes are classified into 133 families (CAZy server, http://www.cazy.org) in which members of each family have a similar structure and catalytic mech...

journal_title：Glycobiology

authors： André-Miral C,Koné FM,Solleux C,Grandjean C,Dion M,Tran V,Tellier C

更新日期：2015-04-01 00:00:00

abstract：:The structure of the O-antigen polysaccharide (PS) from the Shiga-toxin producing Escherichia coli O63 has been elucidated using a combination of bioinformatics, component analyses and NMR spectroscopy. The O-antigen is comprised of tetrasaccharide repeating units with the following structure: →2)-β-d-Quip3N(d-allo-Th...

journal_title：Glycobiology

authors： Ståhle J,Fontana C,Weintraub A,Widmalm G

更新日期：2019-02-01 00:00:00

abstract：:Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine to generate 3-O-sulfated heparan sulfate (HS), which is a rare component in HS from natural sources. We previously reported that 3-O- sulfotransferase isoform 5 (3-OST-5) generates both an antithrombin-binding site to exhibit ...

journal_title：Glycobiology

authors： Chen J,Duncan MB,Carrick K,Pope RM,Liu J

更新日期：2003-11-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...

journal_title：Glycobiology

authors： Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

更新日期：2017-10-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...

journal_title：Glycobiology

authors： Yanagisawa M,Ariga T,Yu RK

更新日期：2006-09-01 00:00:00

abstract：:The human anticoagulant factor, Protein C, is a plasma glycoprotein that has reported anti-ischaemic and anti-inflammatory properties. To explore potential mechanisms for these reported activities, we examined the effect of Protein C on the process of cell adhesion to vascular endothelial cells, which plays a critical...

journal_title：Glycobiology

authors： Grinnell BW,Hermann RB,Yan SB

更新日期：1994-04-01 00:00:00

abstract：:In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...

journal_title：Glycobiology

authors： Ramya TN,Weerapana E,Cravatt BF,Paulson JC

更新日期：2013-02-01 00:00:00

abstract：:Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...

journal_title：Glycobiology

authors： Gaudin JC,Arar C,Monsigny M,Legrand A

更新日期：1997-12-01 00:00:00

abstract：:Affinities of the human blood group glycosyltransferases, alpha-(1-->3)-N-acetylgalactosaminyltransferase (GTA) and alpha-(1-->3)-galactosyltransferase (GTB) for their common acceptor substrate alpha-l-Fucp-(1-->2)-beta-d-Galp-O(CH2)(7)CH3 (1), in the absence and presence of bound uridine 5'-diphosphate (UDP) and Mn2+...

journal_title：Glycobiology

authors： Shoemaker GK,Soya N,Palcic MM,Klassen JS

更新日期：2008-08-01 00:00:00

abstract：:Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...

journal_title：Glycobiology

authors： Stray SJ,Cummings RD,Air GM

更新日期：2000-07-01 00:00:00

abstract：:Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...

journal_title：Glycobiology

authors： Pfeiffer G,Stirm S,Geyer R,Strube KH,Bergwerff AA,Kamerling JP,Vliegenthart JF

更新日期：1992-10-01 00:00:00

abstract：:Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

journal_title：Glycobiology

authors： Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

更新日期：2006-06-01 00:00:00

abstract：:P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

journal_title：Glycobiology

authors： Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

更新日期：1998-07-01 00:00:00

abstract：:Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...

journal_title：Glycobiology

authors： Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue S

更新日期：1999-09-01 00:00:00

abstract：:The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...

journal_title：Glycobiology

authors： Kajiura H,Seki T,Fujiyama K

更新日期：2010-06-01 00:00:00

abstract：:The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...

journal_title：Glycobiology

authors： Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

更新日期：1999-07-01 00:00:00

abstract：:The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...

journal_title：Glycobiology

authors： Janoš P,Kozmon S,Tvaroška I,Koca J

更新日期：2016-07-01 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...

journal_title：Glycobiology

authors： Jin C,Bencúrová M,Borth N,Ferko B,Jensen-Jarolim E,Altmann F,Hantusch B

更新日期：2006-04-01 00:00:00

abstract：:The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...

journal_title：Glycobiology

authors： Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

更新日期：2008-12-01 00:00:00

abstract：:Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...

journal_title：Glycobiology

authors： Kudoh A,Okawa Y,Shibata N

更新日期：2015-01-01 00:00:00

abstract：:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...

journal_title：Glycobiology

authors： de Haan N,Falck D,Wuhrer M

更新日期：2020-03-20 00:00:00

abstract：:In this report we describe a system capable of resolving all of the known unsaturated disaccharides derived from the chondroitin sulphates, dermatan sulphate and hyaluronic acid by chondroitinase digestion. This system is superior to others in that the non-sulphated and mono-, di- and tri-sulphated disaccharides can b...

journal_title：Glycobiology

authors： Zebrower M,Kieras FJ,Heaney-Kieras J

更新日期：1991-06-01 00:00:00