Influenza virus infection of desialylated cells.

abstract：:In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...

journal_title：Glycobiology

authors： Angulo I,Acebrón I,de las Rivas B,Muñoz R,Rodríguez-Crespo I,Menéndez M,García P,Tateno H,Goldstein IJ,Pérez-Agote B,Mancheño JM

更新日期：2011-10-01 00:00:00

abstract：:To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...

journal_title：Glycobiology

authors： Coppa GV,Gabrielli O,Buzzega D,Zampini L,Galeazzi T,Maccari F,Bertino E,Volpi N

更新日期：2011-03-01 00:00:00

abstract：:Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...

journal_title：Glycobiology

authors： Petitou M,Barzu T,Herault JP,Herbert JM

更新日期：1997-04-01 00:00:00

abstract：:Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...

journal_title：Glycobiology

authors： Morelle W,Strecker G

更新日期：1997-09-01 00:00:00

abstract：:Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

journal_title：Glycobiology

authors： Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

更新日期：2006-06-01 00:00:00

abstract：:Murine sperm initiate fertilization by binding to the specialized extracellular matrix of their complementary eggs, known as the zona pellucida. On the basis of data reported in this study, mouse sperm also bind to rabbit erythrocytes with higher affinity than they do to murine eggs. This unusual interaction between a...

journal_title：Glycobiology

authors： Sutton-Smith M,Wong NK,Khoo KH,Wu SW,Yu SY,Patankar MS,Easton R,Lattanzio FA,Morris HR,Dell A,Clark GF

更新日期：2007-06-01 00:00:00

abstract：:The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes re...

journal_title：Glycobiology

authors： Ye L,Zheng X,Zheng H

更新日期：2014-04-01 00:00:00

abstract：:Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...

journal_title：Glycobiology

authors： Pfeiffer G,Stirm S,Geyer R,Strube KH,Bergwerff AA,Kamerling JP,Vliegenthart JF

更新日期：1992-10-01 00:00:00

abstract：:Human noroviruses (HuNoV), members of the family Caliciviridae, are the major cause of acute viral gastroenteritis worldwide. Successful infection is linked to the ability of the protruding (P) domain of the viral capsid to bind histo-blood group antigens (HBGA). Binding to gangliosides plays a major role for many non...

journal_title：Glycobiology

authors： Wegener H,Mallagaray Á,Schöne T,Peters T,Lockhauserbäumer J,Yan H,Uetrecht C,Hansman GS,Taube S

更新日期：2017-11-01 00:00:00

abstract：:Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...

journal_title：Glycobiology

authors： Takahashi S,Sasaki T,Manya H,Chiba Y,Yoshida A,Mizuno M,Ishida H,Ito F,Inazu T,Kotani N,Takasaki S,Takeuchi M,Endo T

更新日期：2001-01-01 00:00:00

abstract：:Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...

journal_title：Glycobiology

authors： Vionnet J,Vann WF

更新日期：2007-07-01 00:00:00

abstract：:Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...

journal_title：Glycobiology

authors： van Wijk XM,Vallen MJ,van de Westerlo EM,Oosterhof A,Hao W,Versteeg EM,Raben J,Wismans RG,Smetsers TF,Dijkman HB,Schalkwijk J,van Kuppevelt TH

更新日期：2012-12-01 00:00:00

abstract：:The human parasitic trematode Schistosoma mansoni has a complex life cycle that includes the freshwater snail Biomphalaria glabrata as intermediate host. Within each stage, the parasite synthesizes a wide array of glycoconjugates, exhibiting, in part, unique carbohydrate structures. In addition, the parasite expresses...

journal_title：Glycobiology

authors： Lehr T,Geyer H,Maass K,Doenhoff MJ,Geyer R

更新日期：2007-01-01 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:The sialic acid-specific cytotoxic lectin viscumin and its recombinant equivalent rViscumin specifically bind to CD75s-gangliosides with terminal Neu5Acα6Galβ4GlcNAc sequence. We, therefore, comparatively analyzed the content of CD75s-gangliosides and closely related iso-CD75s-gangliosides (terminated by Neu5Acα3Galβ4...

journal_title：Glycobiology

authors： Souady J,Hülsewig M,Distler U,Haier J,Denz A,Pilarsky C,Senninger N,Dreisewerd K,Peter-Katalinic J,Müthing J

更新日期：2011-05-01 00:00:00

abstract：:Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha fruit, is a galactose-binding lectin and a potent mitogen of T and B cells. Knowledge obtained from previous studies on the affinity of ALA was limited to molecular and submolecular levels of Galbeta1-->3GalNAc (T) and its derivatives. In the...

journal_title：Glycobiology

authors： Singh T,Chatterjee U,Wu JH,Chatterjee BP,Wu AM

更新日期：2005-01-01 00:00:00

abstract：:Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...

journal_title：Glycobiology

authors： Prasanphanich NS,Luyai AE,Song X,Heimburg-Molinaro J,Mandalasi M,Mickum M,Smith DF,Nyame AK,Cummings RD

更新日期：2014-07-01 00:00:00

abstract：:The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid tre...

journal_title：Glycobiology

authors： Lawrence CW,Little PA,Little BW,Glushka J,van Halbeek H,Alhadeff JA

更新日期：1993-06-01 00:00:00

abstract：:Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currentl...

journal_title：Glycobiology

authors： Tamana S,Promponas VJ

更新日期：2019-05-01 00:00:00

abstract：:A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...

journal_title：Glycobiology

authors： Abe H,Shimma Y,Jigami Y

更新日期：2003-02-01 00:00:00

abstract：:We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...

journal_title：Glycobiology

authors： Hoffmann A,Nimtz M,Conradt HS

更新日期：1997-06-01 00:00:00

abstract：:Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...

journal_title：Glycobiology

authors： Jin C,Bencúrová M,Borth N,Ferko B,Jensen-Jarolim E,Altmann F,Hantusch B

更新日期：2006-04-01 00:00:00

abstract：:Based on chemical analysis, we have previously concluded that the biologically important lipoarabinomannan (LAM) and lipomannan (LM) from Mycobacterium are multiglycosylated forms of the phosphatidylinositol mannosides (PIMs), the characteristic cell envelope mannophosphoinositides of mycobacteria. Using definitive an...

journal_title：Glycobiology

authors： Khoo KH,Dell A,Morris HR,Brennan PJ,Chatterjee D

更新日期：1995-02-01 00:00:00

abstract：:To investigate the developmental role of complex N-linked oligosaccharides, we previously inactivated the mouse Mgat1 gene which encodes UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI). Mgat1-null embryos developed morphogenic abnormalities by embryonic day (E) 9.5 a...

journal_title：Glycobiology

authors： Campbell RM,Metzler M,Granovsky M,Dennis JW,Marth JD

更新日期：1995-07-01 00:00:00

abstract：:The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...

journal_title：Glycobiology

authors： Janoš P,Kozmon S,Tvaroška I,Koca J

更新日期：2016-07-01 00:00:00

abstract：:We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...

journal_title：Glycobiology

authors： Kitova EN,Kitov PI,Bundle DR,Klassen JS

更新日期：2001-07-01 00:00:00

abstract：:Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...

journal_title：Glycobiology

authors： Festari MF,Trajtenberg F,Berois N,Pantano S,Revoredo L,Kong Y,Solari-Saquieres P,Narimatsu Y,Freire T,Bay S,Robello C,Bénard J,Gerken TA,Clausen H,Osinaga E

更新日期：2017-01-01 00:00:00

abstract：:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

journal_title：Glycobiology

authors： Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

更新日期：2004-08-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...

journal_title：Glycobiology

authors： Langsdorf A,Schumacher V,Shi X,Tran T,Zaia J,Jain S,Taglienti M,Kreidberg JA,Fine A,Ai X

更新日期：2011-02-01 00:00:00

abstract：:trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...

journal_title：Glycobiology

authors： Cremona ML,Campetella O,Sánchez DO,Frasch AC

更新日期：1999-06-01 00:00:00