Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans.


:The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.122). Although O-glycans play important roles in vertebrates, much remains to be learned from model organisms such as the free-living nematode Caenorhabditis elegans, which offer many advantages in exploring O-glycan structure/function. Here, we report the cloning and enzymatic characterization of T-synthase from C. elegans (Ce-T-synthase). A putative C. elegans gene for T-synthase, C38H2.2, was identified in GenBank by a BlastP search using the human T-synthase protein sequence. The full-length cDNA for Ce-T-synthase, which was generated by polymerase chain reaction using a C. elegans cDNA library as the template, contains 1170 bp including the stop TAA. The cDNA encodes a protein of 389 amino acids with typical type II membrane topology and a remarkable 42.7% identity to the human T-synthase. Ce-T-synthase has seven Cys residues in the lumenal domain including six conserved Cys residues in all orthologs. The Ce-T-synthase has four potential N-glycosylation sequons, whereas the mammalian orthologs lack N-glycosylation sequons. Only one gene for Ce-T-synthase was identified in the genome-wide search, and it contains eight exons. Promoter analysis of the Ce-T-synthase using green fluorescent protein (GFP) constructs shows that the gene is expressed at all developmental stages and appears to be in all cells. Unexpectedly, only minimal activity was recovered in the recombinant, soluble Ce-T-synthase secreted from a wide variety of mammalian cell lines, whereas robust enzyme activity was recovered in the soluble Ce-T-synthase expressed in Hi-5 insect cells. Vertebrate T-synthase requires the molecular chaperone Cosmc, but our results show that Ce-T-synthase does not require Cosmc and might require invertebrate-specific factors for the formation of the optimally active enzyme. These results show that the Ce-T-synthase is a functional ortholog to the human T-synthase in generating core 1 O-glycans and open new avenues to explore O-glycan function in this model organism.






Ju T,Zheng Q,Cummings RD




Has Abstract


2006-10-01 00:00:00














  • Directed evolution of a remarkably efficient Kdnase from a bacterial neuraminidase.

    abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...


    pub_type: 杂志文章


    authors: Shamsi Kazem Abadi S,Deen MC,Watson JN,Shidmoossavee FS,Bennet AJ

    更新日期:2020-04-20 00:00:00

  • Mac-2: a versatile galactose-binding protein of mammalian tissues.

    abstract::Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...


    pub_type: 杂志文章,评审


    authors: Hughes RC

    更新日期:1994-02-01 00:00:00

  • Structural features in heparin that interact with VEGF165 and modulate its biological activity.

    abstract::The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...


    pub_type: 杂志文章


    authors: Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

    更新日期:1999-07-01 00:00:00

  • Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots.

    abstract::Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...


    pub_type: 杂志文章


    authors: Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

    更新日期:2019-11-20 00:00:00

  • Molecular characterization of beta-trace protein in human serum and urine: a potential diagnostic marker for renal diseases.

    abstract::We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...


    pub_type: 杂志文章


    authors: Hoffmann A,Nimtz M,Conradt HS

    更新日期:1997-06-01 00:00:00

  • Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate.

    abstract::The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...


    pub_type: 杂志文章


    authors: Kakuda S,Sato Y,Tonoyama Y,Oka S,Kawasaki T

    更新日期:2005-02-01 00:00:00

  • Glycan and glycosaminoglycan binding properties of stromal cell-derived factor (SDF)-1alpha.

    abstract::We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...


    pub_type: 杂志文章


    authors: Mbemba E,Gluckman JC,Gattegno L

    更新日期:2000-01-01 00:00:00

  • Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3.

    abstract::Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal orig...


    pub_type: 杂志文章


    authors: Cornillot JD,Pontet M,Dupuy C,Chadli A,Caron M,Joubert-Caron R,Bourin P,Bladier D

    更新日期:1998-05-01 00:00:00

  • Extraction and structural analysis of glycosaminoglycans from formalin-fixed, paraffin-embedded tissues.

    abstract::Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...


    pub_type: 杂志文章


    authors: van Wijk XM,Vallen MJ,van de Westerlo EM,Oosterhof A,Hao W,Versteeg EM,Raben J,Wismans RG,Smetsers TF,Dijkman HB,Schalkwijk J,van Kuppevelt TH

    更新日期:2012-12-01 00:00:00

  • Involvement of gangliosides in glucosamine-induced proliferation decrease of retinal pericytes.

    abstract::The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...


    pub_type: 杂志文章


    authors: Masson E,Wiernsperger N,Lagarde M,El Bawab S

    更新日期:2005-06-01 00:00:00

  • Glycosylation and proteolytic processing of 70 kDa C-terminal recombinant polypeptides of Plasmodium falciparum merozoite surface protein 1 expressed in mammalian cells.

    abstract::The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...


    pub_type: 杂志文章


    authors: Yang S,Nikodem D,Davidson EA,Gowda DC

    更新日期:1999-12-01 00:00:00

  • Prevention of the death of the rat axotomized hypoglossal nerve and promotion of its regeneration by bovine brain gangliosides.

    abstract::We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...


    pub_type: 杂志文章


    authors: Itoh M,Fukumoto S,Baba N,Kuga Y,Mizuno A,Furukawa K

    更新日期:1999-11-01 00:00:00

  • Immunological reactions in response to apicomplexan glycosylphosphatidylinositols.

    abstract::Apicomplexan protozoa are a phylum of parasites that includes pathogens such as Plasmodium, the causative agent of the most severe form of malaria responsible for almost 1 million deaths per year and Toxoplasma gondii causing toxoplasmosis, a disease leading to cerebral meningitis in immunocompromised individuals or t...


    pub_type: 杂志文章,评审


    authors: Debierre-Grockiego F,Schwarz RT

    更新日期:2010-07-01 00:00:00

  • Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively.

    abstract::Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two fo...


    pub_type: 杂志文章


    authors: Bigi A,Morosi L,Pozzi C,Forcella M,Tettamanti G,Venerando B,Monti E,Fusi P

    更新日期:2010-02-01 00:00:00

  • Glycosylation and biological activity of CAMPATH-1H expressed in different cell lines and grown under different culture conditions.

    abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...


    pub_type: 杂志文章


    authors: Lifely MR,Hale C,Boyce S,Keen MJ,Phillips J

    更新日期:1995-12-01 00:00:00

  • Enzymically inactive members of the trans-sialidase family from Trypanosoma cruzi display beta-galactose binding activity.

    abstract::trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...


    pub_type: 杂志文章


    authors: Cremona ML,Campetella O,Sánchez DO,Frasch AC

    更新日期:1999-06-01 00:00:00

  • Characterization of the oligosaccharides assembled on the Pichia pastoris-expressed recombinant aspartic protease.

    abstract::Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...


    pub_type: 杂志文章


    authors: Montesino R,Nimtz M,Quintero O,García R,Falcón V,Cremata JA

    更新日期:1999-10-01 00:00:00

  • The observation of multivalent complexes of Shiga-like toxin with globotriaoside and the determination of their stoichiometry by nanoelectrospray Fourier-transform ion cyclotron resonance mass spectrometry.

    abstract::We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...


    pub_type: 杂志文章


    authors: Kitova EN,Kitov PI,Bundle DR,Klassen JS

    更新日期:2001-07-01 00:00:00

  • Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins.

    abstract::The occurrence of a terminal disialyl motif on mammalian O-glycans is increasingly being identified through recent mass spectrometry (MS)-based glycomic profiling. In most cases, it is carried on simple core 1 structures in which both the galactose and N-acetyl galactosamine can be disialylated. In contrast, a disialy...


    pub_type: 杂志文章


    authors: Wang SH,Tsai CM,Lin KI,Khoo KH

    更新日期:2013-06-01 00:00:00

  • SR proteins and galectins: what's in a name?

    abstract::Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...


    pub_type: 杂志文章,评审


    authors: Haudek KC,Patterson RJ,Wang JL

    更新日期:2010-10-01 00:00:00

  • Mutation of Asn128 to Asp of Phaseolus vulgaris leucoagglutinin (PHA-L) eliminates carbohydrate-binding and biological activity.

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    pub_type: 杂志文章


    authors: Mirkov TE,Chrispeels MJ

    更新日期:1993-12-01 00:00:00

  • Production of N-sulfated polysaccharides using yeast-expressed N-deacetylase/N-sulfotransferase-1 (NDST-1).

    abstract::Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...


    pub_type: 杂志文章


    authors: Saribaş AS,Mobasseri A,Pristatsky P,Chen X,Barthelson R,Hakes D,Wang J

    更新日期:2004-12-01 00:00:00

  • Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells.

    abstract::The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...


    pub_type: 杂志文章


    authors: Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

    更新日期:2008-12-01 00:00:00

  • Reconstructed glycan profile for evaluation of operating status of the endoplasmic reticulum glycoprotein quality control.

    abstract::Glycoprotein oligosaccharides function as tags for protein quality control in the endoplasmic reticulum (ER). Since most of proteins are glycosylated and function only after they are properly folded, glycoprotein glycan profiles in the ER might be useful to analyze various cellular status including diseases. Here, we ...


    pub_type: 杂志文章


    authors: Iwamoto S,Isoyama M,Hirano M,Yamaya K,Ito Y,Matsuo I,Totani K

    更新日期:2013-01-01 00:00:00

  • New oligosaccharyltransferase assay method.

    abstract::We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demons...


    pub_type: 杂志文章


    authors: Kohda D,Yamada M,Igura M,Kamishikiryo J,Maenaka K

    更新日期:2007-11-01 00:00:00

  • Specificity of xenoreactive anti-Gal alpha 1-3Gal IgM for alpha-galactosyl ligands.

    abstract::The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...


    pub_type: 杂志文章


    authors: Parker W,Lateef J,Everett ML,Platt JL

    更新日期:1996-07-01 00:00:00

  • High-level expression of the Endo-beta-N-acetylglucosaminidase F2 gene in E.coli: one step purification to homogeneity.

    abstract::The Endo F2gene was overexpressed in E.coli as a fusion protein joined to the maltose-binding protein. MBP-Endo F2was found in a highly enriched state as insoluble, inactive inclusion bodies. Extraction of the inclusion bodies with 20% acetic acid followed by exhaustive dialysis rendered the fusion protein active and ...


    pub_type: 杂志文章


    authors: Reddy A,Grimwood BG,Plummer TH,Tarentino AL

    更新日期:1998-06-01 00:00:00

  • Identification of two discrete peptide: N-glycanases in Oryzias latipes during embryogenesis.

    abstract::Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...


    pub_type: 杂志文章


    authors: Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue S

    更新日期:1999-09-01 00:00:00

  • Molecular characterization of the cis-prenyltransferase of Giardia lamblia.

    abstract::Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...


    pub_type: 杂志文章


    authors: Grabińska KA,Cui J,Chatterjee A,Guan Z,Raetz CR,Robbins PW,Samuelson J

    更新日期:2010-07-01 00:00:00

  • Purification of CMP-N-acetylneuraminic acid synthetase from bovine anterior pituitary glands.

    abstract::CMP-beta-N-acetylneuraminic acid (CMP-neuNAc) is the substrate for the sialylation of glycoconjugates by sialyltransferases in microbes and higher eukaryotes. CMP-neuNAc synthetase catalyzes the formation of this substrate, CMP-neuNAc, from CTP and neuNAc. In this report we describe the purification of CMP-neuNAc synt...


    pub_type: 杂志文章


    authors: Vionnet J,Concepcion N,Warner T,Zapata G,Hanover J,Vann WF

    更新日期:1999-05-01 00:00:00