Abstract:
:Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two forms, NEU4 long and NEU4 short, differing in the presence of a 12-amino-acid sequence at the N-terminus. Contradictory data are present in the literature about the subcellular distribution of these enzymes, their membrane anchoring mechanism being still unclear. In this work, we investigate the human NEU4 long and NEU4 short membrane anchoring mechanism and their subcellular localization. Protein extraction with Triton X-114 and sodium carbonate and cross-linking experiments demonstrate that both forms of NEU4 are extrinsic membrane proteins, anchored via protein-protein interactions. Moreover, through confocal microscopy and subcellular fractionation, we show that the long form localizes in mitochondria, while the short form is also associated with the endoplasmic reticulum. Finally, mitochondria subfractionation experiments suggest that NEU4 long is bound to the outer mitochondrial membrane.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Bigi A,Morosi L,Pozzi C,Forcella M,Tettamanti G,Venerando B,Monti E,Fusi Pdoi
10.1093/glycob/cwp156subject
Has Abstractpub_date
2010-02-01 00:00:00pages
148-57issue
2eissn
0959-6658issn
1460-2423pii
cwp156journal_volume
20pub_type
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