Monosaccharide and oligosaccharide analysis of isoelectric focusing-separated and blotted granulocyte colony-stimulating factor glycoforms using high-pH anion-exchange chromatography with pulsed amperometric detection.

abstract：:The galectin from Geodia cydonium (GCA) had previously been shown to be involved in regulatory mechanisms of cell sorting and adhesion during reaggregation of allogeneic sponge cells. In this contribution the binding specificity of GCA was established to be GalNAc alpha 1-3GalNAc beta as structural component of Forssm...

journal_title：Glycobiology

authors： Hanisch FG,Baldus SE,Kümmel TA

更新日期：1996-04-01 00:00:00

abstract：:The gut microbiota plays a major role in human health and an alteration in gut microbiota structure and function has been implicated in several diseases. In the colon, mucus covering the epithelium is critical to maintain a homeostatic relationship with the gut microbiota by harbouring a microbial community at safe di...

journal_title：Glycobiology

authors： Bell A,Juge N

更新日期：2020-10-09 00:00:00

abstract：:Multivalency in lectins is a phenomenon that has been discussed at considerable length. The structural basis for the role of multivalency in garlic lectin has been investigated here through computational studies. Biochemical studies have shown that the binding affinity of garlic lectin for high mannose oligosaccharide...

journal_title：Glycobiology

authors： Ramachandraiah G,Chandra NR,Surolia A,Vijayan M

更新日期：2003-11-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...

journal_title：Glycobiology

authors： Muthusamy A,Achur RN,Valiyaveettil M,Madhunapantula SV,Kakizaki I,Bhavanandan VP,Gowda CD

更新日期：2004-07-01 00:00:00

abstract：:The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pa...

journal_title：Glycobiology

authors： Séveno M,Séveno-Carpentier E,Voxeur A,Menu-Bouaouiche L,Rihouey C,Delmas F,Chevalier C,Driouich A,Lerouge P

更新日期：2010-05-01 00:00:00

abstract：:Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...

journal_title：Glycobiology

authors： Petitou M,Barzu T,Herault JP,Herbert JM

更新日期：1997-04-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...

journal_title：Glycobiology

authors： Kim JW,Budzak J,Liu Y,Jégouzo SAF,Drickamer K,Taylor ME

更新日期：2018-08-01 00:00:00

abstract：:The synthesis of Galalpha1-3Gal-terminated oligosaccharides (alpha-Gal) epitopes has been interrupted during the course of evolution, starting with Old World primates. Partial sequences similar to the alpha1,3-galactosyltransferase (alpha1,3GalT) gene, which governs the synthesis of alpha-Gal epitopes, have been detec...

journal_title：Glycobiology

authors： Lantéri M,Giordanengo V,Vidal F,Gaudray P,Lefebvre JC

更新日期：2002-12-01 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...

journal_title：Glycobiology

authors： Peracaula R,Tabarés G,Royle L,Harvey DJ,Dwek RA,Rudd PM,de Llorens R

更新日期：2003-06-01 00:00:00

abstract：:We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...

journal_title：Glycobiology

authors： Hoffmann A,Nimtz M,Conradt HS

更新日期：1997-06-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:Tarin, the Colocasia esculenta lectin from the superfamily of α-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 Å...

journal_title：Glycobiology

authors： Pereira PR,Meagher JL,Winter HC,Goldstein IJ,Paschoalin VM,Silva JT,Stuckey JA

更新日期：2017-01-01 00:00:00

abstract：:Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...

journal_title：Glycobiology

authors： Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue S

更新日期：1999-09-01 00:00:00

abstract：:Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...

journal_title：Glycobiology

authors： Clark SL,Santin AE,Bryant PA,Holman R,Rodnick KJ

更新日期：2013-11-01 00:00:00

abstract：:Schistosoma mansoni soluble egg antigens (SEAs) are crucially involved in modulating the host immune response to infection by S. mansoni. We report that human dendritic cells bind SEAs through the C-type lectin dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN). Monoclonal antibodies against the carbohydrat...

journal_title：Glycobiology

authors： van Die I,van Vliet SJ,Nyame AK,Cummings RD,Bank CM,Appelmelk B,Geijtenbeek TB,van Kooyk Y

更新日期：2003-06-01 00:00:00

abstract：:Streptococcus macedonicus is a Gram positive lactic acid bacterium that is part of the starter flora present in Greek sheep and goat cheeses. The S. macedonicus Sc136 strain produces a high-molecular-mass, highly texturizing exopolysaccharide composed of D-glucose, D-galactose, and N-acetyl-D-glucosamine in the molar ...

journal_title：Glycobiology

authors： Vincent SJ,Faber EJ,Neeser JR,Stingele F,Kamerling JP

更新日期：2001-02-01 00:00:00

abstract：:We report here a method of simultaneously quantifying glucosylceramide (GlcCer) and galactosylceramide (GalCer) by normal-phase HPLC using O-phtalaldehyde derivatives. Treatment with sphingolipid ceramide N-deacylase which converts the cerebrosides in the sample to their lyso-forms was followed by the quantitative lab...

journal_title：Glycobiology

authors： Zama K,Hayashi Y,Ito S,Hirabayashi Y,Inoue T,Ohno K,Okino N,Ito M

更新日期：2009-07-01 00:00:00

abstract：:The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...

journal_title：Glycobiology

authors： Kakuda S,Sato Y,Tonoyama Y,Oka S,Kawasaki T

更新日期：2005-02-01 00:00:00

abstract：:Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...

journal_title：Glycobiology

authors： Festari MF,Trajtenberg F,Berois N,Pantano S,Revoredo L,Kong Y,Solari-Saquieres P,Narimatsu Y,Freire T,Bay S,Robello C,Bénard J,Gerken TA,Clausen H,Osinaga E

更新日期：2017-01-01 00:00:00

abstract：:Cryptococcus neoformans, an opportunistic fungal pathogen, produces a glycan capsule to evade the immune system during infection. This definitive virulence factor is composed mainly of complex polysaccharides, which are made in the secretory pathway by reactions that utilize activated nucleotide sugar precursors. Alth...

journal_title：Glycobiology

authors： Li LX,Ashikov A,Liu H,Griffith CL,Bakker H,Doering TL

更新日期：2017-01-01 00:00:00

abstract：:In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...

journal_title：Glycobiology

authors： Mast SW,Diekman K,Karaveg K,Davis A,Sifers RN,Moremen KW

更新日期：2005-04-01 00:00:00

abstract：:Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currentl...

journal_title：Glycobiology

authors： Tamana S,Promponas VJ

更新日期：2019-05-01 00:00:00

abstract：:Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...

journal_title：Glycobiology

authors： Dall'Olio GM,Jassal B,Montanucci L,Gagneux P,Bertranpetit J,Laayouni H

更新日期：2011-11-01 00:00:00

abstract：:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...

journal_title：Glycobiology

authors： Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter E

更新日期：2005-10-01 00:00:00

abstract：:Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...

journal_title：Glycobiology

authors： Ikarashi K,Fujiwara H,Yamazaki Y,Goto J,Kaneko K,Kato H,Fujii S,Sasaki H,Fukumoto S,Furukawa K,Waki H,Furukawa K

更新日期：2011-10-01 00:00:00

abstract：:Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...

journal_title：Glycobiology

authors： Lopez PH,Aja S,Aoki K,Seldin MM,Lei X,Ronnett GV,Wong GW,Schnaar RL

更新日期：2017-01-01 00:00:00

abstract：:Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...

journal_title：Glycobiology

authors： Suzuki M,Suzuki M,Nakayama J,Suzuki A,Angata K,Chen S,Sakai K,Hagihara K,Yamaguchi Y,Fukuda M

更新日期：2005-09-01 00:00:00

abstract：:Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...

journal_title：Glycobiology

authors： Saribaş AS,Mobasseri A,Pristatsky P,Chen X,Barthelson R,Hakes D,Wang J

更新日期：2004-12-01 00:00:00