Abstract:
:It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We have analyzed GalNAc-carrying peptides of up to 25 amino acids, and could distinguish even neighboring glycosylation sites. This method was also able to localize and characterize disaccharides (e.g., the Thomsen-Friedenreich disaccharide) on MUC1 derived peptides. PSD-MALDI-MS fragment ion patterns were recorded in the positive ion mode from the synthetic peptide TAP25 [(T1aAPPAHGVT9S10APDT14RPAPGS20) T1bAPPA], an overlapping sequence of MUC1 tandem repeats, which was glycosylated with GaINAc in vitro. The glycosylation sites found were either Thr9 or Thr1b in the monoglycosylated, Thr9 and Thr1b in the diglycosylated, and Thr9, Thr1b, and Ser20 in the triglycosylated peptide. A single PSD-MALDI-MS spectrum of the underivatized and uncleaved di- or triglycosylated TAP25 peptide was sufficient to identify the glycosylation sites, thereby distinguishing six potential, partly adjacent, glycosylation sites. The monoglycosylated fraction was found to consist of a mixture of two glycosylated species with the same molecular weight. This was shown by the analysis of proteolytic digests. PSD-MALDI-MS of the resulting peptides right out of the digestion probe was sufficient to identify the Gal-NAc-glycosylation sites as either Thr9 or Thr1b, respectively. Beyond the methodical aspects the results revealed that in vitro glycosylation of the TAP25 peptide with a transferase system from human milk differs from that obtained with a breast cancer cell transferase system.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten Udoi
10.1093/glycob/7.7.881subject
Has Abstractpub_date
1997-10-01 00:00:00pages
881-96issue
7eissn
0959-6658issn
1460-2423journal_volume
7pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.5.517
更新日期:1998-05-01 00:00:00
abstract::Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt061
更新日期:2013-11-01 00:00:00
abstract::Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh133
更新日期:2004-12-01 00:00:00
abstract::Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1193
更新日期:1997-12-01 00:00:00
abstract::Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj071
更新日期:2006-04-01 00:00:00
abstract::The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.12.1183
更新日期:1998-12-01 00:00:00
abstract::All IgG-type antibodies are N-glycosylated in their Fc part at Asn-297. Typically, a fucose residue is attached to the first N-acetylglucosamine of these complex-type N-glycans. Antibodies lacking core fucosylation show a significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and an increased effi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq109
更新日期:2010-12-01 00:00:00
abstract::Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwx056
更新日期:2017-09-01 00:00:00
abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl057
更新日期:2007-01-01 00:00:00
abstract::Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwj080
更新日期:2006-05-01 00:00:00
abstract::The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl033
更新日期:2006-12-01 00:00:00
abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi008
更新日期:2005-03-01 00:00:00
abstract::Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy085
更新日期:2019-01-01 00:00:00
abstract::In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.4.459
更新日期:1994-08-01 00:00:00
abstract::Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm032
更新日期:2007-07-01 00:00:00
abstract::Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy035
更新日期:2018-07-01 00:00:00
abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww019
更新日期:2016-07-01 00:00:00
abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwp027
更新日期:2009-07-01 00:00:00
abstract::An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromatography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.9.759
更新日期:2001-09-01 00:00:00
abstract::Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww111
更新日期:2017-01-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00
abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1089
更新日期:1997-12-01 00:00:00
abstract::P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.7.695
更新日期:1998-07-01 00:00:00
abstract::Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh082
更新日期:2004-08-01 00:00:00
abstract::The mucin-type O-glycome in cancer aberrantly expresses the truncated glycans Tn (GalNAcα1-Ser/Thr) and STn (Neu5Acα2,6GalNAcα1-Ser/Thr). However, the role of Tn and STn in cancer and other diseases is not well understood. Our recent discovery of the self-binding properties (carbohydrate-carbohydrate interactions, CCI...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwy032
更新日期:2018-07-01 00:00:00
abstract::Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq036
更新日期:2010-07-01 00:00:00
abstract::In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt041
更新日期:2013-09-01 00:00:00
abstract::Galbeta1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight-amino-acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabdi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp125
更新日期:2009-12-01 00:00:00
abstract::Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.6.605
更新日期:1991-12-01 00:00:00