A sequencing strategy for the localization of O-glycosylation sites of MUC1 tandem repeats by PSD-MALDI mass spectrometry.

Abstract:

:It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We have analyzed GalNAc-carrying peptides of up to 25 amino acids, and could distinguish even neighboring glycosylation sites. This method was also able to localize and characterize disaccharides (e.g., the Thomsen-Friedenreich disaccharide) on MUC1 derived peptides. PSD-MALDI-MS fragment ion patterns were recorded in the positive ion mode from the synthetic peptide TAP25 [(T1aAPPAHGVT9S10APDT14RPAPGS20) T1bAPPA], an overlapping sequence of MUC1 tandem repeats, which was glycosylated with GaINAc in vitro. The glycosylation sites found were either Thr9 or Thr1b in the monoglycosylated, Thr9 and Thr1b in the diglycosylated, and Thr9, Thr1b, and Ser20 in the triglycosylated peptide. A single PSD-MALDI-MS spectrum of the underivatized and uncleaved di- or triglycosylated TAP25 peptide was sufficient to identify the glycosylation sites, thereby distinguishing six potential, partly adjacent, glycosylation sites. The monoglycosylated fraction was found to consist of a mixture of two glycosylated species with the same molecular weight. This was shown by the analysis of proteolytic digests. PSD-MALDI-MS of the resulting peptides right out of the digestion probe was sufficient to identify the Gal-NAc-glycosylation sites as either Thr9 or Thr1b, respectively. Beyond the methodical aspects the results revealed that in vitro glycosylation of the TAP25 peptide with a transferase system from human milk differs from that obtained with a breast cancer cell transferase system.

journal_name

Glycobiology

journal_title

Glycobiology

authors

Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten U

doi

10.1093/glycob/7.7.881

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

881-96

issue

7

eissn

0959-6658

issn

1460-2423

journal_volume

7

pub_type

杂志文章
  • The expanding beta 4-galactosyltransferase gene family: messages from the databanks.

    abstract::From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/8.5.517

    authors: Lo NW,Shaper JH,Pevsner J,Shaper NL

    更新日期:1998-05-01 00:00:00

  • The initial noncovalent binding of glucose to human hemoglobin in nonenzymatic glycation.

    abstract::Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwt061

    authors: Clark SL,Santin AE,Bryant PA,Holman R,Rodnick KJ

    更新日期:2013-11-01 00:00:00

  • Sialyltransferase mRNA abundances in B cells are strictly controlled, correlated with cognate lectin binding, and differentially responsive to immune signaling in vitro.

    abstract::Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwh133

    authors: Marino JH,Hoffman M,Meyer M,Miller KS

    更新日期:2004-12-01 00:00:00

  • Externalization and binding of galectin-1 on cell surface of K562 cells upon erythroid differentiation.

    abstract::Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/7.8.1193

    authors: Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

    更新日期:1997-12-01 00:00:00

  • Immunoglobulin G specifically binding plant N-glycans with high affinity could be generated in rabbits but not in mice.

    abstract::Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwj071

    authors: Jin C,Bencúrová M,Borth N,Ferko B,Jensen-Jarolim E,Altmann F,Hantusch B

    更新日期:2006-04-01 00:00:00

  • Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi.

    abstract::The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/8.12.1183

    authors: Vandersall-Nairn AS,Merkle RK,O'Brien K,Oeltmann TN,Moremen KW

    更新日期:1998-12-01 00:00:00

  • Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase.

    abstract::All IgG-type antibodies are N-glycosylated in their Fc part at Asn-297. Typically, a fucose residue is attached to the first N-acetylglucosamine of these complex-type N-glycans. Antibodies lacking core fucosylation show a significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and an increased effi...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwq109

    authors: von Horsten HH,Ogorek C,Blanchard V,Demmler C,Giese C,Winkler K,Kaup M,Berger M,Jordan I,Sandig V

    更新日期:2010-12-01 00:00:00

  • Planning, evaluating and vetting receptor signaling studies to assess hyaluronan size-dependence and specificity.

    abstract::Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...

    journal_title:Glycobiology

    pub_type: 杂志文章,评审

    doi:10.1093/glycob/cwx056

    authors: Weigel PH

    更新日期:2017-09-01 00:00:00

  • Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types.

    abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwl057

    authors: Kanda Y,Yamada T,Mori K,Okazaki A,Inoue M,Kitajima-Miyama K,Kuni-Kamochi R,Nakano R,Yano K,Kakita S,Shitara K,Satoh M

    更新日期:2007-01-01 00:00:00

  • Advancing glycomics: implementation strategies at the consortium for functional glycomics.

    abstract::Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...

    journal_title:Glycobiology

    pub_type: 杂志文章,评审

    doi:10.1093/glycob/cwj080

    authors: Raman R,Venkataraman M,Ramakrishnan S,Lang W,Raguram S,Sasisekharan R

    更新日期:2006-05-01 00:00:00

  • Core oligosaccharide structure from the highly phytopathogenic Agrobacterium tumefaciens TT111 and conformational analysis of the putative rhamnan epitope.

    abstract::The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwl033

    authors: De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

    更新日期:2006-12-01 00:00:00

  • Preparation and application of biologically active fluorescent hyaluronan oligosaccharides.

    abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwi008

    authors: Seyfried NT,Blundell CD,Day AJ,Almond A

    更新日期:2005-03-01 00:00:00

  • Galectin-3 binds selectively to the terminal, non-reducing end of β(1→4)-galactans, with overall affinity increasing with chain length.

    abstract::Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwy085

    authors: Miller MC,Zheng Y,Zhou Y,Tai G,Mayo KH

    更新日期:2019-01-01 00:00:00

  • Monosaccharide and oligosaccharide analysis of isoelectric focusing-separated and blotted granulocyte colony-stimulating factor glycoforms using high-pH anion-exchange chromatography with pulsed amperometric detection.

    abstract::In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/4.4.459

    authors: Andersen DC,Goochee CF,Cooper G,Weitzhandler M

    更新日期:1994-08-01 00:00:00

  • Successive glycosyltransfer of sialic acid by Escherichia coli K92 polysialyltransferase in elongation of oligosialic acceptors.

    abstract::Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwm032

    authors: Vionnet J,Vann WF

    更新日期:2007-07-01 00:00:00

  • Novel thioglycoside analogs of α-galactosylceramide stimulate cytotoxicity and preferential Th1 cytokine production by human invariant natural killer T cells.

    abstract::Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwy035

    authors: Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

    更新日期:2018-07-01 00:00:00

  • Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

    abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cww019

    authors: Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

    更新日期:2016-07-01 00:00:00

  • A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.

    abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cws159

    authors: Kawabe K,Tateyama D,Toyoda H,Kawasaki N,Hashii N,Nakao H,Matsumoto S,Nonaka M,Matsumura H,Hirose Y,Morita A,Katayama M,Sakuma M,Kawasaki N,Furue MK,Kawasaki T

    更新日期:2013-03-01 00:00:00

  • Antiganglioside antibodies and their pathophysiological effects on Guillain-Barré syndrome and related disorders--a review.

    abstract::Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...

    journal_title:Glycobiology

    pub_type: 杂志文章,评审

    doi:10.1093/glycob/cwp027

    authors: Kaida K,Ariga T,Yu RK

    更新日期:2009-07-01 00:00:00

  • An ultrasensitive chemical method for polysialic acid analysis.

    abstract::An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromatography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytical...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/11.9.759

    authors: Inoue S,Lin SL,Lee YC,Inoue Y

    更新日期:2001-09-01 00:00:00

  • Revisiting the human polypeptide GalNAc-T1 and T13 paralogs.

    abstract::Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cww111

    authors: Festari MF,Trajtenberg F,Berois N,Pantano S,Revoredo L,Kong Y,Solari-Saquieres P,Narimatsu Y,Freire T,Bay S,Robello C,Bénard J,Gerken TA,Clausen H,Osinaga E

    更新日期:2017-01-01 00:00:00

  • Incorporation of orally applied (13)C-galactose into milk lactose and oligosaccharides.

    abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwj092

    authors: Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

    更新日期:2006-06-01 00:00:00

  • Modulation of the expression of the rabbit galectin-3 gene by p53 and c-Ha-ras proteins and PMA.

    abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/7.8.1089

    authors: Gaudin JC,Arar C,Monsigny M,Legrand A

    更新日期:1997-12-01 00:00:00

  • Delineation of the epitope recognized by an antibody specific for N-glycolylneuraminic acid-containing gangliosides.

    abstract::P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/8.7.695

    authors: Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

    更新日期:1998-07-01 00:00:00

  • The binding of VIP36 and alpha-amylase in the secretory vesicles via high-mannose type glycans.

    abstract::Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwh082

    authors: Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

    更新日期:2004-08-01 00:00:00

  • Tn and STn are members of a family of carbohydrate tumor antigens that possess carbohydrate-carbohydrate interactions.

    abstract::The mucin-type O-glycome in cancer aberrantly expresses the truncated glycans Tn (GalNAcα1-Ser/Thr) and STn (Neu5Acα2,6GalNAcα1-Ser/Thr). However, the role of Tn and STn in cancer and other diseases is not well understood. Our recent discovery of the self-binding properties (carbohydrate-carbohydrate interactions, CCI...

    journal_title:Glycobiology

    pub_type: 杂志文章,评审

    doi:10.1093/glycob/cwy032

    authors: Sletmoen M,Gerken TA,Stokke BT,Burchell J,Brewer CF

    更新日期:2018-07-01 00:00:00

  • Molecular characterization of the cis-prenyltransferase of Giardia lamblia.

    abstract::Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwq036

    authors: Grabińska KA,Cui J,Chatterjee A,Guan Z,Raetz CR,Robbins PW,Samuelson J

    更新日期:2010-07-01 00:00:00

  • α-L-fucosidase from Paenibacillus thiaminolyticus: its hydrolytic and transglycosylation abilities.

    abstract::In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwt041

    authors: Benesová E,Lipovová P,Dvoráková H,Králová B

    更新日期:2013-09-01 00:00:00

  • Caenorhabditis elegans galectins LEC-6 and LEC-1 recognize a chemically synthesized Galbeta1-4Fuc disaccharide unit which is present in Protostomia glycoconjugates.

    abstract::Galbeta1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight-amino-acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabdi...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/cwp125

    authors: Takeuchi T,Nishiyama K,Sugiura K,Takahashi M,Yamada A,Kobayashi S,Takahashi H,Natsugari H,Kasai K

    更新日期:2009-12-01 00:00:00

  • Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.

    abstract::Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...

    journal_title:Glycobiology

    pub_type: 杂志文章

    doi:10.1093/glycob/1.6.605

    authors: Ziegler FD,Trimble RB

    更新日期:1991-12-01 00:00:00