The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode.

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:We expressed recombinant Cys subdomains in COS-7 cells to examine the role of this highly conserved protein domain in mucin biosynthesis. The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/...

journal_title：Glycobiology

authors： Perez-Vilar J,Randell SH,Boucher RC

更新日期：2004-04-01 00:00:00

abstract：:O-antigen (O-polysaccharide), a part of the outer membrane of Gram-negative bacteria, is one of the most variable cell constituents and is related to bacterial virulence. O-antigen diversity is almost entirely due to genetic variations in O-antigen gene clusters. In this study, the O-polysaccharide structures of Salmo...

journal_title：Glycobiology

authors： Liu B,Perepelov AV,Svensson MV,Shevelev SD,Guo D,Senchenkova SN,Shashkov AS,Weintraub A,Feng L,Widmalm G,Knirel YA,Wang L

更新日期：2010-06-01 00:00:00

abstract：:Glycosphingolipids are expressed on the cell membrane and act as important factors in various events that occur across the plasma membrane. Lactosylceramide (LacCer) is synthesized from glucosylceramide and is a common precursor of various glycosphingolipids existing in whole body. Based on the enzyme purification, β1...

journal_title：Glycobiology

authors： Tokuda N,Numata S,Li X,Nomura T,Takizawa M,Kondo Y,Yamashita Y,Hashimoto N,Kiyono T,Urano T,Furukawa K,Furukawa K

更新日期：2013-10-01 00:00:00

abstract：:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...

journal_title：Glycobiology

authors： Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter E

更新日期：2005-10-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:Giardia lamblia, the protist that causes diarrhea, makes an Asn-linked-glycan (N-glycan) precursor that contains just two sugars (GlcNAc(2)) attached by a pyrophosphate linkage to a polyprenol lipid. Because the candidate cis-prenyltransferase of Giardia appears to be more similar to bacterial enzymes than to those of...

journal_title：Glycobiology

authors： Grabińska KA,Cui J,Chatterjee A,Guan Z,Raetz CR,Robbins PW,Samuelson J

更新日期：2010-07-01 00:00:00

abstract：:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

journal_title：Glycobiology

authors： Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

更新日期：2004-08-01 00:00:00

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...

journal_title：Glycobiology

authors： Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

更新日期：2019-11-20 00:00:00

abstract：:F-type lectins are fucolectins with characteristic fucose and calcium-binding sequence motifs and a unique lectin fold (the "F-type" fold). F-type lectins are phylogenetically widespread with selective distribution. Several eukaryotic F-type lectins have been biochemically and structurally characterized, and the F-typ...

journal_title：Glycobiology

authors： Bishnoi R,Khatri I,Subramanian S,Ramya TN

更新日期：2015-08-01 00:00:00

abstract：:Lens culinaris lectin (LCA) is a useful probe for the detection in serum of a core-fucosylated alpha-fetoprotein, called AFP-L3 fraction, which is a well-known marker for the diagnosis and prognosis of hepatocellular carcinoma. Here we performed a systematic quantitative interaction analysis of LCA and its close homol...

journal_title：Glycobiology

authors： Tateno H,Nakamura-Tsuruta S,Hirabayashi J

更新日期：2009-05-01 00:00:00

abstract：:Beta-1,2-linked mannosides from Candida albicans phosphopeptidomannan (PPM) bind to macrophages through a receptor independent from the macrophage alpha-linked mannose receptor and stimulate these cells to secrete immune mediators. Anti-beta-1,2-linked mannoside but not anti-alpha-linked mannoside antibodies produced ...

journal_title：Glycobiology

authors： Jouault T,Fradin C,Dzierszinski F,Borg-Von-Zepelin M,Tomavo S,Corman R,Trinel PA,Kerckaert JP,Poulain D

更新日期：2001-08-01 00:00:00

abstract：:UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...

journal_title：Glycobiology

authors： Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

更新日期：2016-07-01 00:00:00

abstract：:Intestinal maturation can be influenced by intrinsic factors (glucocorticoid hormones) and by extrinsic factors (resident microflora); their relative roles in ontogeny of mouse intestinal trehalase expression, a marker of general gut development, and of beta1,4-galactosyltransferase (beta GT), a marker of glycosyltran...

journal_title：Glycobiology

authors： Nanthakumar NN,Dai D,Meng D,Chaudry N,Newburg DS,Walker WA

更新日期：2005-03-01 00:00:00

abstract：:Lutrophin (LH) consists of an array of isoforms with different charges and bioactivities. This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges. Porcine LH (pLH) was separated into four isoforms by isoelectric focusing (IEF), followed by subu...

journal_title：Glycobiology

authors： Ujihara M,Yamamoto K,Nomura K,Toyoshima S,Demura H,Nakamura Y,Ohmura K,Osawa T

更新日期：1992-06-01 00:00:00

abstract：:Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...

journal_title：Glycobiology

authors： Dall'Olio GM,Jassal B,Montanucci L,Gagneux P,Bertranpetit J,Laayouni H

更新日期：2011-11-01 00:00:00

abstract：:N-Glycans from the nematode Haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the N,N'-diacetylchitobiosyl core, two being on the reducing-terminal proximal GlcNAc and one on the distal core GlcNAc residue. The...

journal_title：Glycobiology

authors： Paschinger K,Wilson IB

更新日期：2015-06-01 00:00:00

abstract：:Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integri...

journal_title：Glycobiology

authors： Chatterjee D,Khoo KH

更新日期：1998-02-01 00:00:00

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00

abstract：:Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...

journal_title：Glycobiology

authors： Smith DF,Rangarajan D

更新日期：1995-03-01 00:00:00

abstract：:We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...

journal_title：Glycobiology

authors： Hoffmann A,Nimtz M,Conradt HS

更新日期：1997-06-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00

abstract：:Angiogenesis is associated with growth, invasion, and metastasis of human solid tumors. Aberrant activation of endothelial cells and induction of microvascular permeability by a vascular endothelial growth factor (VEGF) receptor-2 (VEGFR-2) signaling pathway is observed in pathological angiogenesis including tumor, wo...

journal_title：Glycobiology

authors： Chung TW,Kim SJ,Choi HJ,Kim KJ,Kim MJ,Kim SH,Lee HJ,Ko JH,Lee YC,Suzuki A,Kim CH

更新日期：2009-03-01 00:00:00

abstract：:The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can elicit an immune response or bind to Man receptors, thus reducing their efficacy. Recent studies have...

journal_title：Glycobiology

authors： Hopkins D,Gomathinayagam S,Rittenhour AM,Du M,Hoyt E,Karaveg K,Mitchell T,Nett JH,Sharkey NJ,Stadheim TA,Li H,Hamilton SR

更新日期：2011-12-01 00:00:00

abstract：:We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...

journal_title：Glycobiology

authors： Kotani M,Kawashima I,Ozawa H,Terashima T,Tai T

更新日期：1993-04-01 00:00:00

abstract：:Glycosylation is the most common post-translational modification of serum proteins, and changes in the type and abundance of glycans in human serum have been correlated with a growing number of human diseases. While the glycosylation pattern of human serum is well studied, little is known about the profiles of other m...

journal_title：Glycobiology

authors： Behrens AJ,Duke RM,Petralia LM,Harvey DJ,Lehoux S,Magnelli PE,Taron CH,Foster JM

更新日期：2018-11-01 00:00:00

abstract：:Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...

journal_title：Glycobiology

authors： Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

更新日期：2001-07-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:Isolation of glycopeptides utilizing hydrogen bonding between glycopeptide glycans and a carbohydrate-gel matrix in the organic phase is useful for site-specific characterization of oligosaccharides of glycoproteins, when combined with mass spectrometry. In this study, recovery of glycopeptides was improved by includi...

journal_title：Glycobiology

authors： Tajiri M,Yoshida S,Wada Y

更新日期：2005-12-01 00:00:00