A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.

abstract：:In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...

journal_title：Glycobiology

authors： Ramya TN,Weerapana E,Cravatt BF,Paulson JC

更新日期：2013-02-01 00:00:00

abstract：:We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demons...

journal_title：Glycobiology

authors： Kohda D,Yamada M,Igura M,Kamishikiryo J,Maenaka K

更新日期：2007-11-01 00:00:00

abstract：:Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...

journal_title：Glycobiology

authors： Gesteira TF,Coulson-Thomas VJ

更新日期：2018-11-01 00:00:00

abstract：:The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...

journal_title：Glycobiology

authors： Janoš P,Kozmon S,Tvaroška I,Koca J

更新日期：2016-07-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:Cytotrophoblasts are the specialized epithelial cells of the placenta. During the first trimester, a subpopulation of chorionic villus cytotrophoblasts differentiates along an invasive pathway and penetrates the maternal endometrium, decidua and spiral arterioles. Cytotrophoblast invasiveness declines rapidly during t...

journal_title：Glycobiology

authors： Moss L,Prakobphol A,Wiedmann TW,Fisher SJ,Damsky CH

更新日期：1994-10-01 00:00:00

abstract：:Epithelial cells in the lining of the intestines play critical roles in maintaining homeostasis while challenged by dynamic and sudden changes in luminal contents. Given the high density of glycosylation that encompasses their extracellular surface, environmental changes may lead to extensive reorganization of membran...

journal_title：Glycobiology

authors： Park D,Xu G,Barboza M,Shah IM,Wong M,Raybould H,Mills DA,Lebrilla CB

更新日期：2017-09-01 00:00:00

abstract：:Type A Pasteurella multocida, an animal pathogen, employs a hyaluronan [HA] capsule to avoid host defenses. PmHAS, the 972-residue membrane-associated hyaluronan synthase, catalyzes the transfer of both GlcNAc and GlcUA to form the HA polymer. To define the catalytic and membrane-associated domains, pmHAS mutants were...

journal_title：Glycobiology

authors： Jing W,DeAngelis PL

更新日期：2000-09-01 00:00:00

abstract：:Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (Gal-1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of ...

journal_title：Glycobiology

authors： Camby I,Le Mercier M,Lefranc F,Kiss R

更新日期：2006-11-01 00:00:00

abstract：:In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. The truncated glycosylation exposes cryptic peptide epitopes that can be recognized by antibodies. Since these immunogenic regions are cancer specific, they represent ideal targets for therapeutic antibodies. We investigated ...

journal_title：Glycobiology

authors： Movahedin M,Brooks TM,Supekar NT,Gokanapudi N,Boons GJ,Brooks CL

更新日期：2017-07-01 00:00:00

abstract：:We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...

journal_title：Glycobiology

authors： Shoji H,Nishi N,Hirashima M,Nakamura T

更新日期：2002-03-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...

journal_title：Glycobiology

authors： Yanagisawa M,Ariga T,Yu RK

更新日期：2006-09-01 00:00:00

abstract：:The occurrence of a terminal disialyl motif on mammalian O-glycans is increasingly being identified through recent mass spectrometry (MS)-based glycomic profiling. In most cases, it is carried on simple core 1 structures in which both the galactose and N-acetyl galactosamine can be disialylated. In contrast, a disialy...

journal_title：Glycobiology

authors： Wang SH,Tsai CM,Lin KI,Khoo KH

更新日期：2013-06-01 00:00:00

abstract：:Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...

journal_title：Glycobiology

authors： Prasanphanich NS,Luyai AE,Song X,Heimburg-Molinaro J,Mandalasi M,Mickum M,Smith DF,Nyame AK,Cummings RD

更新日期：2014-07-01 00:00:00

abstract：:The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.1...

journal_title：Glycobiology

authors： Ju T,Zheng Q,Cummings RD

更新日期：2006-10-01 00:00:00

abstract：:Glycoinositolphospholipids (GIPLs) were isolated from promastigotes of the lizard parasites Leishmania adleri by phenol/water extraction. Phosphoinositol oligosaccharides were liberated by mild alkaline hydrolysis, purified by gel filtration and high pH anion exchange chromatography, and characterized by methylation a...

journal_title：Glycobiology

authors： Previato JO,Jones C,Wait R,Routier F,Saraiva E,Mendonça-Previato L

更新日期：1997-07-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00

abstract：:Knowledge of the identity, synthesis and secretion of beta-galactoside-binding lectins by leukocytes is of importance because lactosaminoglycans present at the leukocyte cell surface may be physiologically significant lectin receptors that could mediate autocrine or paracrine functions and/or cell adhesion. This paper...

journal_title：Glycobiology

authors： Sharma A,DiCioccio RA,Allen HJ

更新日期：1992-08-01 00:00:00

abstract：:The Gram-negative bacterium Campylobacter jejuni 81116 (Penner serotype HS:6) has a class E lipooligosaccharide (LOS) biosynthesis locus containing 19 genes, which encode for 11 putative glycosyltransferases, 1 lipid A acyltransferase and 7 enzymes thought to be involved in the biosynthesis of dideoxyhexosamine (ddHex...

journal_title：Glycobiology

authors： Li ZZ,Riegert AS,Goneau MF,Cunningham AM,Vinogradov E,Li J,Schoenhofen IC,Thoden JB,Holden HM,Gilbert M

更新日期：2017-04-01 00:00:00

abstract：:Despite the controversy regarding the importance of protein N-linked glycosylation in species of the genus Plasmodium, genes potentially encoding core subunits of the oligosaccharyltransferase (OST) complex have already been characterized in completely sequenced genomes of malaria parasites. Nevertheless, the currentl...

journal_title：Glycobiology

authors： Tamana S,Promponas VJ

更新日期：2019-05-01 00:00:00

abstract：:Intestinal maturation can be influenced by intrinsic factors (glucocorticoid hormones) and by extrinsic factors (resident microflora); their relative roles in ontogeny of mouse intestinal trehalase expression, a marker of general gut development, and of beta1,4-galactosyltransferase (beta GT), a marker of glycosyltran...

journal_title：Glycobiology

authors： Nanthakumar NN,Dai D,Meng D,Chaudry N,Newburg DS,Walker WA

更新日期：2005-03-01 00:00:00

abstract：:N-Glycans from the nematode Haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the N,N'-diacetylchitobiosyl core, two being on the reducing-terminal proximal GlcNAc and one on the distal core GlcNAc residue. The...

journal_title：Glycobiology

authors： Paschinger K,Wilson IB

更新日期：2015-06-01 00:00:00

abstract：:Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...

journal_title：Glycobiology

authors： Pashov A,MacLeod S,Saha R,Perry M,VanCott TC,Kieber-Emmons T

更新日期：2005-10-01 00:00:00

abstract：:The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...

journal_title：Glycobiology

authors： Masson E,Wiernsperger N,Lagarde M,El Bawab S

更新日期：2005-06-01 00:00:00

abstract：:N-Acetylglucosaminyltransferase-IV (GnT-IV) has two isoenzymes, GnT-IVa and GnT-IVb, which initiate the GlcNAcbeta1-4 branch synthesis on the Manalpha1-3 arm of the N-glycan core thereby increasing N-glycan branch complexity and conferring endogenous lectin binding epitopes. To elucidate the physiological significance...

journal_title：Glycobiology

authors： Takamatsu S,Antonopoulos A,Ohtsubo K,Ditto D,Chiba Y,Le DT,Morris HR,Haslam SM,Dell A,Marth JD,Taniguchi N

更新日期：2010-01-01 00:00:00

abstract：:Shigella flexneri is the major human pathogen causing shigellosis. O-antigens of all S. flexneri serotypes (except for serotype 6) share the →2)-α-l-Rhap(III)-(1 → 2)-α-l-Rhap(II)-(1 → 3)-α-l-Rhap(I)-(1 → 3)-β-d-GlcpNAc-(1→ basic O-unit, whereas differences between the serotypes are conferred by phage-encoded glucosyl...

journal_title：Glycobiology

authors： Knirel YA,Lan R,Senchenkova SN,Wang J,Shashkov AS,Wang Y,Perepelov AV,Xiong Y,Xu J,Sun Q

更新日期：2013-04-01 00:00:00

abstract：:In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...

journal_title：Glycobiology

authors： Mast SW,Diekman K,Karaveg K,Davis A,Sifers RN,Moremen KW

更新日期：2005-04-01 00:00:00

abstract：:Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...

journal_title：Glycobiology

authors： Lee-Sundlov MM,Ashline DJ,Hanneman AJ,Grozovsky R,Reinhold VN,Hoffmeister KM,Lau JT

更新日期：2017-01-01 00:00:00

abstract：:Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...

journal_title：Glycobiology

authors： Kaida K,Ariga T,Yu RK

更新日期：2009-07-01 00:00:00

abstract：:Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...

journal_title：Glycobiology

authors： Marino JH,Hoffman M,Meyer M,Miller KS

更新日期：2004-12-01 00:00:00