Abstract:
:Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B lymphocytes respond to specific immune signals in vitro with tightly regulated changes in the sialic acid composition of the cell surface glycocalyx. These changes are both quantitative and qualitative in nature and are apparently regulated at both the transcriptional and posttranscriptional levels. We used lectin binding and flow cytometry combined with relative real-time PCR to show that MAH and PNA binding are tightly correlated with the abundance of ST3Gal IV and ST3Gal I mRNA, respectively, under several different conditions of B cell stimulation. Finally, we show that although SNA binding and the expression of ST6Gal I coding sequence are not tightly correlated, there is a clear differential control of 5'UTR exon usage in response to different immune signals.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Marino JH,Hoffman M,Meyer M,Miller KSdoi
10.1093/glycob/cwh133subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1265-74issue
12eissn
0959-6658issn
1460-2423pii
cwh133journal_volume
14pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn090
更新日期:2008-12-01 00:00:00
abstract::N-Glycanase 1, encoded by NGLY1, catalyzes the deglycosylation of misfolded N-linked glycoproteins retrotranslocated into the cytosol. We identified nine cases with mutations in NGLY1. The patients show developmental delay, seizures, peripheral neuropathy, abnormal liver function and alacrima (absence of tears). The m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv024
更新日期:2015-08-01 00:00:00
abstract::Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh082
更新日期:2004-08-01 00:00:00
abstract::Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj071
更新日期:2006-04-01 00:00:00
abstract::Lens culinaris lectin (LCA) is a useful probe for the detection in serum of a core-fucosylated alpha-fetoprotein, called AFP-L3 fraction, which is a well-known marker for the diagnosis and prognosis of hepatocellular carcinoma. Here we performed a systematic quantitative interaction analysis of LCA and its close homol...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp016
更新日期:2009-05-01 00:00:00
abstract::Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha fruit, is a galactose-binding lectin and a potent mitogen of T and B cells. Knowledge obtained from previous studies on the affinity of ALA was limited to molecular and submolecular levels of Galbeta1-->3GalNAc (T) and its derivatives. In the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh144
更新日期:2005-01-01 00:00:00
abstract::Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two fo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp156
更新日期:2010-02-01 00:00:00
abstract::The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj087
更新日期:2006-05-01 00:00:00
abstract::Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.2.231
更新日期:1997-03-01 00:00:00
abstract::Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy050
更新日期:2018-08-01 00:00:00
abstract::Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi071
更新日期:2005-09-01 00:00:00
abstract::Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq215
更新日期:2011-11-01 00:00:00
abstract::Glycation of immunoglobulin G (IgG) can result from incubation with a reducing sugar in vitro or during circulation in vivo. Upon injection of a recombinantly produced human therapeutic IgG into humans, changes in the glycation levels could be observed as a function of circulation time. Mass changes on the individual ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr141
更新日期:2012-02-01 00:00:00
abstract::Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwp027
更新日期:2009-07-01 00:00:00
abstract::The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz009
更新日期:2019-05-01 00:00:00
abstract::The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm003
更新日期:2007-04-01 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.6.581
更新日期:1999-06-01 00:00:00
abstract::Serglycin with a green fluorescent protein tag (SG-GFP) expressed in epithelial Madin-Darby canine kidney cells is secreted mainly (85%) into the apical medium, but the glycosaminoglycan (GAG) chains on the SG-GFP protein core secreted basolaterally (15%) carry most of the sulfate added during biosynthesis (Tveit et a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj075
更新日期:2006-04-01 00:00:00
abstract::We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.2.177
更新日期:1996-03-01 00:00:00
abstract::The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.3.227
更新日期:1999-03-01 00:00:00
abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl057
更新日期:2007-01-01 00:00:00
abstract::Heparan sulfates (HSs) exert critical regulatory actions on many proteins, including growth factors, and are essential for normal development. Variations in their specific sulfation patterns are known to regulate binding and signaling of fibroblast growth factors (FGFs) via tyrosine kinase receptors (FGFRs). We previo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf072
更新日期:2002-11-01 00:00:00
abstract::Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl017
更新日期:2006-10-01 00:00:00
abstract::Since the turn of the century, cancer therapy has undergone a transformation in terms of new treatment modalities and renewed optimism in achieving long-lived tumor control and even cure. This is, in large part, thanks to the widespread incorporation of monoclonal antibodies (mAbs) into standard treatment regimens. Th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwy043
更新日期:2018-09-01 00:00:00
abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/5.2.161
更新日期:1995-03-01 00:00:00
abstract::Since plants are emerging as an important system for the expression of recombinant glycoproteins, especially those intended for therapeutic purposes, it is important to scrutinize to what extent glycans harbored by mammalian glycoproteins produced in transgenic plants differ from their natural counterpart. We report h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.4.365
更新日期:1999-04-01 00:00:00
abstract::Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww098
更新日期:2017-01-01 00:00:00
abstract::N-Glycans from the nematode Haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the N,N'-diacetylchitobiosyl core, two being on the reducing-terminal proximal GlcNAc and one on the distal core GlcNAc residue. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv015
更新日期:2015-06-01 00:00:00