Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide.

abstract：:A genetic basis for the biosynthetic assembly of the globotetraose containing lipopolysaccharide (LPS) of Haemophilus influenzae strain RM118 (Rd) was determined by structural analysis of LPS derived from mutant strains. We have previously shown that the parent strain RM118 elaborates a population of LPS molecules mad...

journal_title：Glycobiology

authors： Hood DW,Cox AD,Wakarchuk WW,Schur M,Schweda EK,Walsh SL,Deadman ME,Martin A,Moxon ER,Richards JC

更新日期：2001-11-01 00:00:00

abstract：:Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...

journal_title：Glycobiology

authors： Takahashi S,Sasaki T,Manya H,Chiba Y,Yoshida A,Mizuno M,Ishida H,Ito F,Inazu T,Kotani N,Takasaki S,Takeuchi M,Endo T

更新日期：2001-01-01 00:00:00

abstract：:Glycoprotein oligosaccharides function as tags for protein quality control in the endoplasmic reticulum (ER). Since most of proteins are glycosylated and function only after they are properly folded, glycoprotein glycan profiles in the ER might be useful to analyze various cellular status including diseases. Here, we ...

journal_title：Glycobiology

authors： Iwamoto S,Isoyama M,Hirano M,Yamaya K,Ito Y,Matsuo I,Totani K

更新日期：2013-01-01 00:00:00

abstract：:The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...

journal_title：Glycobiology

authors： Kajiura H,Seki T,Fujiyama K

更新日期：2010-06-01 00:00:00

abstract：:The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...

journal_title：Glycobiology

authors： Vandersall-Nairn AS,Merkle RK,O'Brien K,Oeltmann TN,Moremen KW

更新日期：1998-12-01 00:00:00

abstract：:Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...

journal_title：Glycobiology

authors： Miller MC,Zheng Y,Zhou Y,Tai G,Mayo KH

更新日期：2019-01-01 00:00:00

abstract：:Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. Here we describe the construction of a stable recom...

journal_title：Glycobiology

authors： Mattila P,Räbinä J,Hortling S,Helin J,Renkonen R

更新日期：2000-10-01 00:00:00

abstract：:Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...

journal_title：Glycobiology

authors： Ikarashi K,Fujiwara H,Yamazaki Y,Goto J,Kaneko K,Kato H,Fujii S,Sasaki H,Fukumoto S,Furukawa K,Waki H,Furukawa K

更新日期：2011-10-01 00:00:00

abstract：:Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...

journal_title：Glycobiology

authors： Haudek KC,Patterson RJ,Wang JL

更新日期：2010-10-01 00:00:00

abstract：:The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...

journal_title：Glycobiology

authors： Campbell MP,Abrahams JL,Rapp E,Struwe WB,Costello CE,Novotny M,Ranzinger R,York WS,Kolarich D,Rudd PM,Kettner C

更新日期：2019-05-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...

journal_title：Glycobiology

authors： Wojczyk BS,Takahashi N,Levy MT,Andrews DW,Abrams WR,Wunner WH,Spitalnik SL

更新日期：2005-06-01 00:00:00

abstract：:The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...

journal_title：Glycobiology

authors： Kakuda S,Sato Y,Tonoyama Y,Oka S,Kawasaki T

更新日期：2005-02-01 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00

abstract：:The Gram-negative bacterium Campylobacter jejuni 81116 (Penner serotype HS:6) has a class E lipooligosaccharide (LOS) biosynthesis locus containing 19 genes, which encode for 11 putative glycosyltransferases, 1 lipid A acyltransferase and 7 enzymes thought to be involved in the biosynthesis of dideoxyhexosamine (ddHex...

journal_title：Glycobiology

authors： Li ZZ,Riegert AS,Goneau MF,Cunningham AM,Vinogradov E,Li J,Schoenhofen IC,Thoden JB,Holden HM,Gilbert M

更新日期：2017-04-01 00:00:00

abstract：:Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...

journal_title：Glycobiology

authors： Hughes RC

更新日期：1994-02-01 00:00:00

abstract：:We report here a method of simultaneously quantifying glucosylceramide (GlcCer) and galactosylceramide (GalCer) by normal-phase HPLC using O-phtalaldehyde derivatives. Treatment with sphingolipid ceramide N-deacylase which converts the cerebrosides in the sample to their lyso-forms was followed by the quantitative lab...

journal_title：Glycobiology

authors： Zama K,Hayashi Y,Ito S,Hirabayashi Y,Inoue T,Ohno K,Okino N,Ito M

更新日期：2009-07-01 00:00:00

abstract：:Cancer-associated glycan structures can be both tumor markers and engines of disease progression. The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan. Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological an...

journal_title：Glycobiology

authors： Venturi G,Gomes Ferreira I,Pucci M,Ferracin M,Malagolini N,Chiricolo M,Dall'Olio F

更新日期：2019-09-20 00:00:00

abstract：:Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...

journal_title：Glycobiology

authors： Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

更新日期：2019-11-20 00:00:00

abstract：:Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...

journal_title：Glycobiology

authors： Bencúrová M,Hemmer W,Focke-Tejkl M,Wilson IB,Altmann F

更新日期：2004-05-01 00:00:00

abstract：:trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...

journal_title：Glycobiology

authors： Cremona ML,Campetella O,Sánchez DO,Frasch AC

更新日期：1999-06-01 00:00:00

abstract：:The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The pres...

journal_title：Glycobiology

authors： Thomsson KA,Prakobphol A,Leffler H,Reddy MS,Levine MJ,Fisher SJ,Hansson GC

更新日期：2002-01-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00

abstract：:Tarin, the Colocasia esculenta lectin from the superfamily of α-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 Å...

journal_title：Glycobiology

authors： Pereira PR,Meagher JL,Winter HC,Goldstein IJ,Paschoalin VM,Silva JT,Stuckey JA

更新日期：2017-01-01 00:00:00

abstract：:Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benef...

journal_title：Glycobiology

authors： Porfirio S,Archer-Hartmann S,Moreau GB,Ramakrishnan G,Haque R,Kirkpatrick BD,Petri WA,Azadi P

更新日期：2020-09-28 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:Trehalose synthase (TreS) catalyzes the reversible conversion of maltose into trehalose in mycobacteria as one of three biosynthetic pathways to this nonreducing disaccharide. Given the importance of trehalose to survival of mycobacteria, there has been considerable interest in understanding the enzymes involved in it...

journal_title：Glycobiology

authors： Caner S,Nguyen N,Aguda A,Zhang R,Pan YT,Withers SG,Brayer GD

更新日期：2013-09-01 00:00:00

abstract：:alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...

journal_title：Glycobiology

authors： Hirano K,Ziak M,Kamoshita K,Sukenaga Y,Kametani S,Shiga Y,Roth J,Akanuma H

更新日期：2000-12-01 00:00:00

abstract：:The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of the intra- and extracellular protein fractions revealed the presence of multiple transferrin glycoforms with molecular weights lower than t...

journal_title：Glycobiology

authors： Ailor E,Takahashi N,Tsukamoto Y,Masuda K,Rahman BA,Jarvis DL,Lee YC,Betenbaugh MJ

更新日期：2000-08-01 00:00:00