Abstract:
:Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that Galectin-1 (Gal-1), a carbohydrate-binding protein, induces myoblast differentiation and fusion in vitro, suggesting a potential role for this mammalian lectin in muscle regenerative processes in vivo. However, the expression and localization of Gal-1 in vivo during muscle injury and repair are unclear. We report the expression and localization of Gal-1 during degenerative-regenerative processes in vivo using two models of muscular dystrophy and muscle injury. Gal-1 expression increased significantly during muscle degeneration in the murine mdx and in the canine Golden Retriever Muscular Dystrophy animal models. Compulsory exercise of mdx mouse, which intensifies degeneration, also resulted in sustained Gal-1 levels. Furthermore, muscle injury of wild-type C57BL/6 mice, induced by BaCl(2) treatment, also resulted in a marked increase in Gal-1 levels. Increased Gal-1 levels appeared to localize both inside and outside the muscle fibers with significant extracellular Gal-1 colocalized with infiltrating CD45(+) leukocytes. By contrast, regenerating muscle tissue showed a marked decrease in Gal-1 to baseline levels. These results demonstrate significant regulation of Gal-1 expression in vivo and suggest a potential role for Gal-1 in muscle homeostasis and repair.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Cerri DG,Rodrigues LC,Stowell SR,Araujo DD,Coelho MC,Oliveira SR,Bizario JC,Cummings RD,Dias-Baruffi M,Costa MCdoi
10.1093/glycob/cwn079subject
Has Abstractpub_date
2008-11-01 00:00:00pages
842-50issue
11eissn
0959-6658issn
1460-2423pii
cwn079journal_volume
18pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingd...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz092
更新日期:2020-02-19 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.3.227
更新日期:1999-03-01 00:00:00
abstract::Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu091
更新日期:2015-01-01 00:00:00
abstract::Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt061
更新日期:2013-11-01 00:00:00
abstract::Mycobacterium tuberculosis lipoarabinomannan (LAM) and biosynthetically related lipoglycans and glycans play an important role in host-pathogen interactions. Therefore, the elucidation of the complete biosynthetic pathways of these important molecules is expected to afford novel therapeutic targets. The characterizati...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv061
更新日期:2015-11-01 00:00:00
abstract::Glycosylation determines essential biological functions of epithelial mucins in health and disease. We report on the influence of glycosylation of the immunodominant DTR motif of MUC1 on its antigenicity. Sets of novel glycopeptides were synthesized that enabled us to examine sole and combined effects of peptide lengt...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh090
更新日期:2004-08-01 00:00:00
abstract::trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.6.581
更新日期:1999-06-01 00:00:00
abstract::Elevated ammonium concentrations in the medium of cultivated cells have been shown to increase the intracellular levels of uridine-5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphospho-N-acetylgalactosamine (UDP-GalNAc; Ryll et al., 1994). These sugar nucleotides are substrates for glycosyltransferase...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.2.125
更新日期:1999-02-01 00:00:00
abstract::We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.2.177
更新日期:1996-03-01 00:00:00
abstract::The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx039
更新日期:2017-09-01 00:00:00
abstract::To facilitate deciphering the information content in the glycome, thin film-coated photoactivatable surfaces were applied for covalent immobilization of glycans, glycoconjugates, or lectins in microarray formats. Light-induced immobilization of a series of bacterial exopolysaccharides on photoactivatable dextran-coate...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh143
更新日期:2005-01-01 00:00:00
abstract::The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/1.5.487
更新日期:1991-11-01 00:00:00
abstract::Macrophages are major cells of inflammatory process and take part in a large number of physiological and pathological processes. According to tissue environment, they can polarize into pro-inflammatory (M1) or alternative (M2) cells. Although many evidences have hinted to a potential role of cell-surface glycosaminogl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu137
更新日期:2015-05-01 00:00:00
abstract::The galectin from Geodia cydonium (GCA) had previously been shown to be involved in regulatory mechanisms of cell sorting and adhesion during reaggregation of allogeneic sponge cells. In this contribution the binding specificity of GCA was established to be GalNAc alpha 1-3GalNAc beta as structural component of Forssm...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.3.321
更新日期:1996-04-01 00:00:00
abstract::The synthesis of Galalpha1-3Gal-terminated oligosaccharides (alpha-Gal) epitopes has been interrupted during the course of evolution, starting with Old World primates. Partial sequences similar to the alpha1,3-galactosyltransferase (alpha1,3GalT) gene, which governs the synthesis of alpha-Gal epitopes, have been detec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf087
更新日期:2002-12-01 00:00:00
abstract::Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.6.587
更新日期:2000-06-01 00:00:00
abstract::Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.6.873
更新日期:1994-12-01 00:00:00
abstract::Poly-N-acetyllactosamine extensions on N- and O-linked glycans are increasingly recognized as biologically important structural features, but access to these structures has not been widely available. Here, we report a detailed substrate specificity and catalytic efficiency of the bacterial β3-N-acetylglucosaminyltrans...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws101
更新日期:2012-11-01 00:00:00
abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.8.813
更新日期:1995-12-01 00:00:00
abstract::Schistosoma mansoni soluble egg antigens (SEAs) are crucially involved in modulating the host immune response to infection by S. mansoni. We report that human dendritic cells bind SEAs through the C-type lectin dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN). Monoclonal antibodies against the carbohydrat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg052
更新日期:2003-06-01 00:00:00
abstract::Since the turn of the century, cancer therapy has undergone a transformation in terms of new treatment modalities and renewed optimism in achieving long-lived tumor control and even cure. This is, in large part, thanks to the widespread incorporation of monoclonal antibodies (mAbs) into standard treatment regimens. Th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwy043
更新日期:2018-09-01 00:00:00
abstract::There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwv102
更新日期:2016-03-01 00:00:00
abstract::The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.7.683
更新日期:1995-10-01 00:00:00
abstract::The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww010
更新日期:2016-07-01 00:00:00
abstract::alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.12.1283
更新日期:2000-12-01 00:00:00
abstract::Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww111
更新日期:2017-01-01 00:00:00
abstract::Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.9.887
更新日期:1999-09-01 00:00:00
abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.6.535
更新日期:1992-12-01 00:00:00
abstract::Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...
journal_title:Glycobiology
pub_type: 信件
doi:10.1093/glycob/cwl003
更新日期:2006-09-01 00:00:00