Abstract:
:Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in therapy to cure severe anemia. The structure of the glycan chains of this rHuEpo slightly differ of those of the urinary human Epo (uHuEpo), considered as the natural Epo molecule. In an attempt to produce a rHuEpo as close as possible to the uHuEpo, Epo gene was expressed in a human lymphoblastoid cell line, named RPMI 1788. In order to fully characterize the Epo-RPMI, structural characterizations of the protein skeleton as well as glycan chains were undergone. As expected, the amino acid sequence of the Epo-RPMI conformed to that of uHuEpo. Surprisingly, the structure of some N-glycan chains, as mainly determined by ESI-MS, revealed some unusual characteristics. Thus, 80% of N-glycans possess a bisecting GlcNAc residue, 25% bear a second fucose residue which is present, in a large part, in a sialyl Le(x)motif, and 13% contain more than three LacNAc repeats (up to five per molecule). Despite these unusual structural characteristics, the data concerning the in vitro and in vivo biological activities were not impaired when compared to Epo-CHO and uHuEpo.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Cointe D,Béliard R,Jorieux S,Leroy Y,Glacet A,Verbert A,Bourel D,Chirat Fdoi
10.1093/glycob/10.5.511subject
Has Abstractpub_date
2000-05-01 00:00:00pages
511-9issue
5eissn
0959-6658issn
1460-2423pii
cwd056journal_volume
10pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws161
更新日期:2013-03-01 00:00:00
abstract::Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that G...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn079
更新日期:2008-11-01 00:00:00
abstract::β-Glucans possess broad immunomodulatory properties, including activation of innate immune functions such as oxidative burst activity. The differential roles of complement receptor type 3 (CR3) and Dectin-1, the known β-glucan receptors, and their associated signaling pathways in the generation of oxidative burst indu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu005
更新日期:2014-04-01 00:00:00
abstract::Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two fo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp156
更新日期:2010-02-01 00:00:00
abstract::We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.1.21
更新日期:2000-01-01 00:00:00
abstract::The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of the intra- and extracellular protein fractions revealed the presence of multiple transferrin glycoforms with molecular weights lower than t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.8.837
更新日期:2000-08-01 00:00:00
abstract::In renal cell carcinoma (RCC), the presence of higher gangliosides correlates with systematic metastasis. Disialosyl globopentaosylceramide (DSGb5) was identified previously as one of the major gangliosides from RCC tissues. Siglec-7 (sialic acid-binding Ig-like lectin-7), expressed on natural killer (NK) cells as an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq116
更新日期:2010-11-01 00:00:00
abstract::We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.653
更新日期:1994-10-01 00:00:00
abstract::In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. The truncated glycosylation exposes cryptic peptide epitopes that can be recognized by antibodies. Since these immunogenic regions are cancer specific, they represent ideal targets for therapeutic antibodies. We investigated ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww131
更新日期:2017-07-01 00:00:00
abstract::Genetic polymorphism studies in humans provide unique opportunities to understand human biology and the mechanisms of diseases. Correlations between polymorphisms in the genes encoding human Siglecs and various diseases have been reported. Leading examples, such as the CD33 polymorphism associated with late-onset Alzh...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwu043
更新日期:2014-09-01 00:00:00
abstract::The human anticoagulant factor, Protein C, is a plasma glycoprotein that has reported anti-ischaemic and anti-inflammatory properties. To explore potential mechanisms for these reported activities, we examined the effect of Protein C on the process of cell adhesion to vascular endothelial cells, which plays a critical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.221
更新日期:1994-04-01 00:00:00
abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1089
更新日期:1997-12-01 00:00:00
abstract::Two kinds of molecules, one recognizing the sialo-/asialo-N-acetyllactosamine structures and the other recognizing the Lewis X structure in a divalent cation-independent manner, were detected on the head of boar sperm prepared from cauda epididymis by fluorescence-labeled or biotinylated dextran-based multivalent olig...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.4.313
更新日期:2001-04-01 00:00:00
abstract::The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg024
更新日期:2003-06-01 00:00:00
abstract::Cancer-associated glycan structures can be both tumor markers and engines of disease progression. The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan. Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological an...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz053
更新日期:2019-09-20 00:00:00
abstract::Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww098
更新日期:2017-01-01 00:00:00
abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf059
更新日期:2002-08-01 00:00:00
abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::At weaning, the intestinal mucosa surface glycans change from predominantly sialylated to fucosylated. Intestinal adaptation from milk to solid food is regulated by intrinsic and extrinsic factors. The contribution by glucocorticoid, an intrinsic factor, and colonization by microbiota, an extrinsic factor, was measure...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt050
更新日期:2013-10-01 00:00:00
abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl057
更新日期:2007-01-01 00:00:00
abstract::The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn097
更新日期:2008-12-01 00:00:00
abstract::Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benef...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa028
更新日期:2020-09-28 00:00:00
abstract::Circulatory protein glycosylation is a biomarker of multiple disease and inflammatory states and has been applied in the clinic for liver dysfunction, heart disease and diabetes. With the notable exception of antibodies, the liver produces most of the circulatory glycoproteins, including the acute phase proteins relea...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz096
更新日期:2020-04-20 00:00:00
abstract::Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1193
更新日期:1997-12-01 00:00:00
abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi008
更新日期:2005-03-01 00:00:00
abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.5.395
更新日期:2001-05-01 00:00:00
abstract::The Gram-negative bacterium Campylobacter jejuni 81116 (Penner serotype HS:6) has a class E lipooligosaccharide (LOS) biosynthesis locus containing 19 genes, which encode for 11 putative glycosyltransferases, 1 lipid A acyltransferase and 7 enzymes thought to be involved in the biosynthesis of dideoxyhexosamine (ddHex...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww136
更新日期:2017-04-01 00:00:00
abstract::O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu017
更新日期:2014-06-01 00:00:00
abstract::Elevated ammonium concentrations in the medium of cultivated cells have been shown to increase the intracellular levels of uridine-5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphospho-N-acetylgalactosamine (UDP-GalNAc; Ryll et al., 1994). These sugar nucleotides are substrates for glycosyltransferase...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.2.125
更新日期:1999-02-01 00:00:00
abstract::Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz058
更新日期:2019-10-21 00:00:00