Sialic acid glycoengineering using N-acetylmannosamine and sialic acid analogs.

abstract：:Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...

journal_title：Glycobiology

authors： Malik RK,Ghurye RR,Lawrence-Watt DJ,Stewart HJ

更新日期：2009-12-01 00:00:00

abstract：:beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. Although a number of reports concerning tumor metastasis-related glycoproteins that contain beta1-6 GlcNAc branching have appeared, the precise f...

journal_title：Glycobiology

authors： Ihara S,Miyoshi E,Nakahara S,Sakiyama H,Ihara H,Akinaga A,Honke K,Dickson RB,Lin CY,Taniguchi N

更新日期：2004-02-01 00:00:00

abstract：:Circulatory protein glycosylation is a biomarker of multiple disease and inflammatory states and has been applied in the clinic for liver dysfunction, heart disease and diabetes. With the notable exception of antibodies, the liver produces most of the circulatory glycoproteins, including the acute phase proteins relea...

journal_title：Glycobiology

authors： Oswald DM,Jones MB,Cobb BA

更新日期：2020-04-20 00:00:00

abstract：:The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...

journal_title：Glycobiology

authors： Gagneux P,Varki A

更新日期：1999-08-01 00:00:00

abstract：:Shigella flexneri is the major human pathogen causing shigellosis. O-antigens of all S. flexneri serotypes (except for serotype 6) share the →2)-α-l-Rhap(III)-(1 → 2)-α-l-Rhap(II)-(1 → 3)-α-l-Rhap(I)-(1 → 3)-β-d-GlcpNAc-(1→ basic O-unit, whereas differences between the serotypes are conferred by phage-encoded glucosyl...

journal_title：Glycobiology

authors： Knirel YA,Lan R,Senchenkova SN,Wang J,Shashkov AS,Wang Y,Perepelov AV,Xiong Y,Xu J,Sun Q

更新日期：2013-04-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium responsible for serious enteric infections that occur mainly in the terminal ileum and colon. High interest in Shigella, as a human pathogen, is driven by its antibiotic resistance and the necessity to develop a vaccine against its infections. Vaccines of the last generat...

journal_title：Glycobiology

authors： Molinaro A,Silipo A,Castro CD,Sturiale L,Nigro G,Garozzo D,Bernardini ML,Lanzetta R,Parrilli M

更新日期：2008-03-01 00:00:00

abstract：:Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...

journal_title：Glycobiology

authors： Hosoi T,Imai Y,Irimura T

更新日期：1998-08-01 00:00:00

abstract：:In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...

journal_title：Glycobiology

authors： Benesová E,Lipovová P,Dvoráková H,Králová B

更新日期：2013-09-01 00:00:00

abstract：:We show by nanoelectrospray ionization (nanoES) Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) that it is possible to observe oligosaccharide-protein complexes with dissociation constants in the millimolar range, such as P(k) trisaccharide (globotriaoside) complexed with the Shiga-like toxin (...

journal_title：Glycobiology

authors： Kitova EN,Kitov PI,Bundle DR,Klassen JS

更新日期：2001-07-01 00:00:00

abstract：:The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...

journal_title：Glycobiology

authors： Kajiura H,Seki T,Fujiyama K

更新日期：2010-06-01 00:00:00

abstract：:Heparin/heparan sulfates (H/HS) are ubiquitous biopolymers that interact with many proteins to induce a range of biological functions. Unfortunately, how these biopolymers recognize their preferred protein targets remain poorly understood. It is suggested that computational simulations offer attractive avenues but a n...

journal_title：Glycobiology

authors： Nagarajan B,Sankaranarayanan NV,Desai UR

更新日期：2020-07-16 00:00:00

abstract：:Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...

journal_title：Glycobiology

authors： Bhat UR,Carlson RW

更新日期：1992-12-01 00:00:00

abstract：:Neisseria meningitidis (Nm) is a leading cause of bacterial meningitis and sepsis. A key feature in pathogenicity is the capsular polysaccharide (CPS) that prevents complement activation and thus supports bacterial survival in the host. Twelve serogroups characterized by immunologically and structurally different CPSs...

journal_title：Glycobiology

authors： Fiebig T,Berti F,Freiberger F,Pinto V,Claus H,Romano MR,Proietti D,Brogioni B,Stummeyer K,Berger M,Vogel U,Costantino P,Gerardy-Schahn R

更新日期：2014-02-01 00:00:00

abstract：:Ganglioside GM3 (Siaα2-3Galβ1-4Glcβ1-1Cer) has been known to participate in insulin signaling by regulating the association of the insulin receptor in caveolae microdomains (lipid rafts), which is essential for the execution of the complete insulin metabolic signaling in adipocytes. Macrophage-secreted factors includi...

journal_title：Glycobiology

authors： Nagafuku M,Sato T,Sato S,Shimizu K,Taira T,Inokuchi J

更新日期：2015-03-01 00:00:00

abstract：:Heparan sulfates (HSs) exert critical regulatory actions on many proteins, including growth factors, and are essential for normal development. Variations in their specific sulfation patterns are known to regulate binding and signaling of fibroblast growth factors (FGFs) via tyrosine kinase receptors (FGFRs). We previo...

journal_title：Glycobiology

authors： Ford-Perriss M,Guimond SE,Greferath U,Kita M,Grobe K,Habuchi H,Kimata K,Esko JD,Murphy M,Turnbull JE

更新日期：2002-11-01 00:00:00

abstract：:Glycosylation is the most common post-translational modification of serum proteins, and changes in the type and abundance of glycans in human serum have been correlated with a growing number of human diseases. While the glycosylation pattern of human serum is well studied, little is known about the profiles of other m...

journal_title：Glycobiology

authors： Behrens AJ,Duke RM,Petralia LM,Harvey DJ,Lehoux S,Magnelli PE,Taron CH,Foster JM

更新日期：2018-11-01 00:00:00

abstract：:Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...

journal_title：Glycobiology

authors： Raman R,Venkataraman M,Ramakrishnan S,Lang W,Raguram S,Sasisekharan R

更新日期：2006-05-01 00:00:00

abstract：:In addition to sialic acid, bacteria produce several other nonulosonic acids, including legionaminic acid (Leg). This has exactly the same stereochemistry as sialic acid, with the added features of 9-deoxy and 7-amino groups. In order to explore the biological effects of replacing sialic acid residues (Neu5Ac) in glyc...

journal_title：Glycobiology

authors： Watson DC,Leclerc S,Wakarchuk WW,Young NM

更新日期：2011-01-01 00:00:00

abstract：:Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...

journal_title：Glycobiology

authors： Bridges CG,Ahmed SP,Kang MS,Nash RJ,Porter EA,Tyms AS

更新日期：1995-03-01 00:00:00

abstract：:Bacterial protein glycosylation systems from varying species have been functionally reconstituted in Escherichia coli. Both N- and O-linked glycosylation pathways, in which the glycans are first assembled onto lipid carriers and subsequently transferred to acceptor proteins by an oligosaccharyltransferase (OTase), hav...

journal_title：Glycobiology

authors： Gebhart C,Ielmini MV,Reiz B,Price NL,Aas FE,Koomey M,Feldman MF

更新日期：2012-07-01 00:00:00

abstract：:Schistosoma mansoni soluble egg antigens (SEAs) are crucially involved in modulating the host immune response to infection by S. mansoni. We report that human dendritic cells bind SEAs through the C-type lectin dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN). Monoclonal antibodies against the carbohydrat...

journal_title：Glycobiology

authors： van Die I,van Vliet SJ,Nyame AK,Cummings RD,Bank CM,Appelmelk B,Geijtenbeek TB,van Kooyk Y

更新日期：2003-06-01 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives which were initiated by the Xyl-MU acting as a primer were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xy...

journal_title：Glycobiology

authors： Tazawa T,Takagaki K,Matsuya H,Nakamura T,Sasaki M,Endo M

更新日期：1998-09-01 00:00:00

abstract：:We have recently demonstrated that the mannose-binding lectins, namely banana lectin (BL) and garlic lectin (GL), interacted with the insulin receptors on M210B4 cells--an established mesenchymal cell line of murine marrow origin--and initiate mitogen-activated protein kinase kinase (MEK)-dependent extracellular signa...

journal_title：Glycobiology

authors： Bajaj M,Hinge A,Limaye LS,Gupta RK,Surolia A,Kale VP

更新日期：2011-04-01 00:00:00

abstract：:Since the turn of the century, cancer therapy has undergone a transformation in terms of new treatment modalities and renewed optimism in achieving long-lived tumor control and even cure. This is, in large part, thanks to the widespread incorporation of monoclonal antibodies (mAbs) into standard treatment regimens. Th...

journal_title：Glycobiology

authors： Dalziel M,Beers SA,Cragg MS,Crispin M

更新日期：2018-09-01 00:00:00

abstract：:Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...

journal_title：Glycobiology

authors： Stray SJ,Cummings RD,Air GM

更新日期：2000-07-01 00:00:00

abstract：:The glycobiology of the cestodes, a class of parasitic flatworms, is still largely unexplored. An important cestode species is Echinococcus granulosus, the tissue-dwelling larval stage of which causes hydatid disease. The E. granulosus larva is protected from the host by a massive mucin-based extracellular matrix term...

journal_title：Glycobiology

authors： Lin G,Todeschini AR,Koizumi A,Neves JL,González H,Dematteis S,Hada N,Previato JO,Ferreira F,Mendonça-Previato L,Díaz A

更新日期：2013-04-01 00:00:00

abstract：:Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...

journal_title：Glycobiology

authors： Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

更新日期：2019-11-20 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...

journal_title：Glycobiology

authors： Shamsi Kazem Abadi S,Deen MC,Watson JN,Shidmoossavee FS,Bennet AJ

更新日期：2020-04-20 00:00:00