Abstract:
:β-Trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophysical studies on a β-trefoil lectin from Entamoeba histolytica (EntTref), which exists as a mixture of monomers and dimers in solution. Further, we have determined the affinities of EntTref for rhamnose, galactose and different galactose-linked sugars. We obtained the crystal structure of EntTref in a sugar-free form (EntTref_apo) and a rhamnose-bound form (EntTref_rham). A novel Cys residue-mediated dimerization was revealed in the crystal structure of EntTref_apo while the structure of EntTref_rham provided the structural basis for the recognition of rhamnose by a β-trefoil lectin for the first time. To the best of our knowledge, this is the only report of the structural, functional and biophysical characterization of a β-trefoil lectin from a protozoan source and the first report of Cys-mediated dimerization in this class of lectins.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Khan F,Kurre D,Suguna Kdoi
10.1093/glycob/cwaa001subject
Has Abstractpub_date
2020-07-20 00:00:00pages
474-488issue
7eissn
0959-6658issn
1460-2423pii
5710858journal_volume
30pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::In renal cell carcinoma (RCC), the presence of higher gangliosides correlates with systematic metastasis. Disialosyl globopentaosylceramide (DSGb5) was identified previously as one of the major gangliosides from RCC tissues. Siglec-7 (sialic acid-binding Ig-like lectin-7), expressed on natural killer (NK) cells as an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq116
更新日期:2010-11-01 00:00:00
abstract::We tested the importance of the aspartate-any residue-aspartate (DXD) motif for the enzymatic activity and nucleotide binding capacity of the Golgi glycosyltransferase GM2 synthase. We prepared point mutations of the motif, which is found in the sequence 352-VLWVDDDFV, and analyzed cells that stably expressed the muta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.3.217
更新日期:2001-03-01 00:00:00
abstract::Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh030
更新日期:2004-03-01 00:00:00
abstract::Macrophages are major cells of inflammatory process and take part in a large number of physiological and pathological processes. According to tissue environment, they can polarize into pro-inflammatory (M1) or alternative (M2) cells. Although many evidences have hinted to a potential role of cell-surface glycosaminogl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu137
更新日期:2015-05-01 00:00:00
abstract::Herein the biodistributions and in vivo kinetics of chemically prepared neoglycoproteins are reviewed. Chemical methods can be used to conjugate various mono- and oligosaccharides onto a protein surface. The kinetics and organ-specific accumulation profiles of these glycoconjugates, which are introduced through intrav...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cww038
更新日期:2016-08-01 00:00:00
abstract::The deletion of the protein mannosyltransferase 1 gene (PMT1) of Saccharomyces cerevisiae results in viable cells. O-Mannosylation of proteins is reduced to about half of the value in comparison to wild-type cells. In order to distinguish between the the PMT1 gene product (= Pmt1p) and residual transferase activity, a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.1.77
更新日期:1995-02-01 00:00:00
abstract::Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp161
更新日期:2010-02-01 00:00:00
abstract::The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq011
更新日期:2010-05-01 00:00:00
abstract::Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine to generate 3-O-sulfated heparan sulfate (HS), which is a rare component in HS from natural sources. We previously reported that 3-O- sulfotransferase isoform 5 (3-OST-5) generates both an antithrombin-binding site to exhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg101
更新日期:2003-11-01 00:00:00
abstract::We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp057
更新日期:2009-08-01 00:00:00
abstract::Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp084
更新日期:2009-09-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq133
更新日期:2011-02-01 00:00:00
abstract::Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy050
更新日期:2018-08-01 00:00:00
abstract::The sialic acid-specific cytotoxic lectin viscumin and its recombinant equivalent rViscumin specifically bind to CD75s-gangliosides with terminal Neu5Acα6Galβ4GlcNAc sequence. We, therefore, comparatively analyzed the content of CD75s-gangliosides and closely related iso-CD75s-gangliosides (terminated by Neu5Acα3Galβ4...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq200
更新日期:2011-05-01 00:00:00
abstract::Mammalian alpha1,6-fucosyltransferase (FUT8) catalyses the transfer of a fucose residue from a donor substrate, guanosine 5'-diphosphate-beta-L-fucose to the reducing terminal N-acetylglucosamine (GlcNAc) of the core structure of an asparagine-linked oligosaccharide. Alpha1,6-fucosylation, also referred to as core fuc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl079
更新日期:2007-05-01 00:00:00
abstract::Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.5.475
更新日期:1993-10-01 00:00:00
abstract::The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx039
更新日期:2017-09-01 00:00:00
abstract::Heparin/heparan sulfates (H/HS) are ubiquitous biopolymers that interact with many proteins to induce a range of biological functions. Unfortunately, how these biopolymers recognize their preferred protein targets remain poorly understood. It is suggested that computational simulations offer attractive avenues but a n...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa015
更新日期:2020-07-16 00:00:00
abstract::We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.4.499
更新日期:1997-06-01 00:00:00
abstract::The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.5.499
更新日期:1996-07-01 00:00:00
abstract::Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (Gal-1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of ...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwl025
更新日期:2006-11-01 00:00:00
abstract::The structure of the O-antigen polysaccharide (PS) from the Shiga-toxin producing Escherichia coli O63 has been elucidated using a combination of bioinformatics, component analyses and NMR spectroscopy. The O-antigen is comprised of tetrasaccharide repeating units with the following structure: →2)-β-d-Quip3N(d-allo-Th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy098
更新日期:2019-02-01 00:00:00
abstract::Affinities of the human blood group glycosyltransferases, alpha-(1-->3)-N-acetylgalactosaminyltransferase (GTA) and alpha-(1-->3)-galactosyltransferase (GTB) for their common acceptor substrate alpha-l-Fucp-(1-->2)-beta-d-Galp-O(CH2)(7)CH3 (1), in the absence and presence of bound uridine 5'-diphosphate (UDP) and Mn2+...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn043
更新日期:2008-08-01 00:00:00
abstract::The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww010
更新日期:2016-07-01 00:00:00
abstract::Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh077
更新日期:2004-07-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh016
更新日期:2004-02-01 00:00:00
abstract::The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm003
更新日期:2007-04-01 00:00:00
abstract::A genetic basis for the biosynthetic assembly of the globotetraose containing lipopolysaccharide (LPS) of Haemophilus influenzae strain RM118 (Rd) was determined by structural analysis of LPS derived from mutant strains. We have previously shown that the parent strain RM118 elaborates a population of LPS molecules mad...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.11.957
更新日期:2001-11-01 00:00:00
abstract::L-Selectin is a lectin-like receptor on lymphocytes which mediates their attachment to high endothelial venules (HEV) within lymph nodes. Previous work has identified HEV-associated endothelial ligands for L-selectin as sialylated, fucosylated and sulphated glycoproteins of approximately 50 kDa and approximately 90 kD...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.4.373
更新日期:1992-08-01 00:00:00
abstract::The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.1...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl008
更新日期:2006-10-01 00:00:00