Abstract:
:Bacterium-host interactions are important for diverse ecological settings including pathogenicity and symbiosis. Electron cryotomography is a powerful method for studying the macromolecular complexes that mediate such interactions in situ. The main limitation of electron cryotomography is its restriction to relatively thin samples such as individual bacterial cells. Cryo-focused ion beam milling was recently proposed as a solution to the thickness limitation. This approach allows the artifact-free thinning of biological specimens for subsequent imaging in the transmission electron microscope. By enabling near-native imaging of bacteria inside their eukaryotic host, this combination of techniques promotes the integration of data from structural biology and infection biology. Therefore, electron cryotomography associated with cryo-focused ion beam milling holds great potential for establishing multiscale models of cell-cell interactions from the atomic, to the cellular and to the intercellular scale.
journal_name
Curr Opin Microbioljournal_title
Current opinion in microbiologyauthors
Medeiros JM,Böck D,Pilhofer Mdoi
10.1016/j.mib.2017.12.006subject
Has Abstractpub_date
2018-06-01 00:00:00pages
62-68eissn
1369-5274issn
1879-0364pii
S1369-5274(17)30200-Xjournal_volume
43pub_type
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