Abstract:
PURPOSE:The purpose of the present study was to apply noninvasive methods for monitoring regeneration and mechanical properties of tissue-engineered cartilage in vivo at different growth stages using MR elastography (MRE). METHODS:Three types of scaffolds, including silk, collagen, and gelatin seeded by human mesenchymal stem cells, were implanted subcutaneously in mice and imaged at 9.4T where the shear stiffness and transverse MR relaxation time (T2 ) were measured for the regenerating constructs for 8 wk. An MRE phase contrast spin echo-based sequence was used for collecting MRE images. At the conclusion of the in vivo study, constructs were excised and transcript levels of cartilage-specific genes were quantitated using reverse-transcription polymerase chain reaction. RESULTS:Tissue-engineered constructs showed a cartilage-like construct with progressive tissue formation characterized by increase in shear stiffness and decrease in T2 that can be correlated with increased cartilage transcript levels including aggrecan, type II collagen, and cartilage oligomeric matrix protein after 8 wk of in vivo culture. CONCLUSION:Altogether, the outcome of this research demonstrates the feasibility of MRE and MRI for noninvasive monitoring of engineered cartilage construct's growth after implantation and provides noninvasive biomarkers for regeneration, which may be translated into treatment of tissue defects.
journal_name
Magn Reson Medjournal_title
Magnetic resonance in medicineauthors
Khalilzad-Sharghi V,Han Z,Xu H,Othman SFdoi
10.1002/mrm.25745subject
Has Abstractpub_date
2016-03-01 00:00:00pages
1209-17issue
3eissn
0740-3194issn
1522-2594journal_volume
75pub_type
杂志文章abstract::A technique for noninvasively quantifying the concentration of sodium ((23) Na) ions was applied to the study of ischemic stroke. (23) Na-magnetic resonance imaging techniques have shown considerable potential for measuring subtle changes in ischemic tissue, although studies to date have suffered primarily from poor s...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.23031
更新日期:2012-03-01 00:00:00
abstract::MRI has become an important tool to noninvasively assess global and regional cardiac function, infarct size, or myocardial blood flow in surgically or genetically modified mouse models of human heart disease. Constraints on scan time due to sensitivity to general anesthesia in hemodynamically compromised mice frequent...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22605
更新日期:2011-01-01 00:00:00
abstract::Longitudinal water proton relaxation rates of methemoglobin solutions show a strong dependence on temperature and pH. The increase of the relaxation rates with temperature is associated with shortened exchange lifetime of the coordinated water molecule. An accurate measurement of the relaxation rate of methemoglobin s...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910330613
更新日期:1995-06-01 00:00:00
abstract::The aim of this work was to identify ventricular shape-based biomarkers in MR images to discriminate between patients with Alzheimer's disease (AD) and healthy elderly. Clinical MR images were collected for 58 patients and 28 age-matched healthy controls. After normalizing all the images the ventricular cerebrospinal ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21471
更新日期:2008-02-01 00:00:00
abstract:PURPOSE:To investigate the use of shielded-coaxial-cable (SCC) coils as elements for multi-channel receive-only and transceive arrays for 7T human MRI and to compare their performance with equivalently sized conventional loop coils. METHODS:The SCC coil element consists of a coaxial loop with interrupted central condu...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.27964
更新日期:2020-03-01 00:00:00
abstract::It is well known that when compared to conventional spin-echo (CSE) imaging for equivalent effective echo times, fast spin-echo (FSE) imaging experiments yield higher signal intensities for coupled spin systems, such as that for lipid. One hypothesis put forth for this phenomenon is the removal of scalar coupling-base...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910350409
更新日期:1996-04-01 00:00:00
abstract::Four-dimensional (4D) flow imaging has been used to study flow patterns and pathophysiology, usually focused on specific thoracic vessels and cardiac chambers. Whole-heart 4D flow at high measurement accuracy covering the entire thoracic cardiovascular system would be desirable to simplify and improve hemodynamic asse...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22090
更新日期:2009-10-01 00:00:00
abstract::A method for volume selective proton spectroscopy is presented based on a multiecho sequence with short refocusing interval tcp. It is demonstrated, that by appropriate choice of tcp on the order of 4-6 ms, signals from overlapping multiplets like the glutamine and glutamate (Glu/Gln) resonances in spectra of the huma...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910370603
更新日期:1997-06-01 00:00:00
abstract::This paper describes a method for correcting the chemical-shift artifacts in 19F NMR imaging of perfluoroctylbromide emulsion (PFOB) by utilizing the two spectral peaks of PFOB which have a long T2 value in conjunction with the Dixon method. Corrected images are obtained from the magnitude of the measured images using...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910230206
更新日期:1992-02-01 00:00:00
abstract::Reduction of image-to-image signal fluctuations and imaging artifacts is important for fMRI studies. The imaging artifacts caused by respiration, cardiac pulsation, and gross head motion and the suppression of these artifacts have been studied for many years. However, the artifacts caused by eye movement and their eff...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910380407
更新日期:1997-10-01 00:00:00
abstract::This work aimed to investigate the effects of administering Gd-DTPA on the BOLD effect, and to determine the feasibility of using this approach to measure fractional changes in blood volume and blood oxygenation on neuronal activation during a visual paradigm. A linear relationship between cortical R(2)(*) and the int...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.10340
更新日期:2003-01-01 00:00:00
abstract::Three-dimensional (3D) coronary imaging has the potential to overcome problems resulting from vessel tortuosity and to reduce partial volume effects. With these techniques, however, acquisition times are long and respiratory motion artifacts problematical. This work describes the development of a method that applies p...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/(sici)1522-2594(199903)41:3<555::aid-mrm19
更新日期:1999-03-01 00:00:00
abstract::Myocardial blood flow varies during the cardiac cycle in response to pulsatile changes in epicardial circulation and cyclical variation in myocardial tension. First-pass assessment of myocardial perfusion by dynamic contrast-enhanced MRI is one of the most challenging applications of MRI because of the spatial and tem...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22538
更新日期:2010-12-01 00:00:00
abstract::Two-dimensional 1H-31P correlation spectroscopy and relaxation time measurements of intact diseased pancreas were performed in order to identify an unknown peak appearing at -0.18 +/- 0.04 ppm in a 31P NMR spectrum of taurocholate-induced experimental acute pancreatitis in rats. The signal was identified as a solubili...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910100111
更新日期:1989-04-01 00:00:00
abstract:PURPOSE:Sensitivity encoding (SENSE) reconstruction of multiband echo planar imaging (EPI) may cause artifacts when simultaneously excited slices require different phase correction to remove the EPI-specific ghost shifted by half of the matrix size (N). We propose a simplified solution of this problem that combines SEN...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25915
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:The correlation between glutamine metabolism and oncogene expression in cancers has led to a renewed interest in the role of glutamine in cancer cell survival. Hyperpolarized [5-(13) C]glutamine is evaluated as a potential biomarker for noninvasive metabolic measurements of drug response in prostate cancer cell...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25360
更新日期:2015-06-01 00:00:00
abstract::The water proton resonance frequency (PRF) is temperature dependent and can thus be used for magnetic resonance (MR) thermometry. Since lipid proton resonance frequencies do not depend on temperature, fat suppression is essential for PRF-based temperature mapping. The efficacy of echo-shifted (TE > TR) gradient-echo i...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/(sici)1522-2594(199907)42:1<53::aid-mrm9>3
更新日期:1999-07-01 00:00:00
abstract::A new parallel imaging technique was implemented which can result in reduced image acquisition times in MRI. MR data is acquired in parallel using an array of receiver coils and then reconstructed simultaneously with multiple processors. The method requires the initial estimation of the 2D sensitivity profile of each ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/1522-2594(200008)44:2<301::aid-mrm18>3.0.c
更新日期:2000-08-01 00:00:00
abstract::When developing new techniques for NMR imaging and in vivo spectroscopy it is a major advantage to be able to calculate the magnetization slice profiles, at any point during a pulse sequence in the presence of a field gradient. While this has frequently been done by treating the problem as a number of discrete resonan...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910050511
更新日期:1987-11-01 00:00:00
abstract::The purpose of the study was to test a newly developed, MR-compatible, liquid nitrogen-cooled cryoprobe. The probe has an outer diameter of 3.5 mm and was specifically developed for percutaneous, MR-guided, interstitial cryotherapy. The probe was inserted percutaneously into the livers of 10 rabbits. The cryotherapy p...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910390304
更新日期:1998-03-01 00:00:00
abstract::Multislice, two-dimensional phosphorus 31 spectroscopic imaging (SI) of human brain was performed in 15 normal volunteers on a 3-Tesla magnetic resonance system. Images of free magnesium concentrations and pH as well as phosphoesters, inorganic phosphate, phosphocreatine, and adenosine triphosphate (ATP), were calcula...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/(sici)1522-2594(199902)41:2<400::aid-mrm26
更新日期:1999-02-01 00:00:00
abstract::Changes in the exchange rate of water across the blood-brain barrier, denoted k(w), may indicate blood-brain barrier dysfunction before the leakage of large-molecule contrast agents is observable. A previously proposed approach for measuring k(w) is to use diffusion-weighted arterial spin labeling to measure the vascu...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.23104
更新日期:2012-05-01 00:00:00
abstract::Three-dimensional magnetization-prepared (MP) MR angiography (MRA) was applied to the carotid and vertebral arteries by using ECG triggering, a slab selective RF inversion pulse, centric phase encoding and acquisition during diastole. Both theoretically and experimentally, the MP MRA sequence was shown to perform well...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910370219
更新日期:1997-02-01 00:00:00
abstract::We present new diffusion phantoms dedicated to the study and validation of high-angular-resolution diffusion imaging (HARDI) models. The phantom design permits the application of imaging parameters that are typically employed in studies of the human brain. The phantoms were made of small-diameter acrylic fibers, chose...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21789
更新日期:2008-12-01 00:00:00
abstract::Manganese-enhanced MRI has previously been used for visualization of brain architecture and functional mapping of neural pathways. The present work investigated the potential of manganese-enhanced MRI for noninvasive imaging of salivary glands in living subjects. Marked shortening of T(1) was observed in salivary glan...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22452
更新日期:2010-09-01 00:00:00
abstract::The investigation of flow behavior in coronary arteries is of great importance for an understanding of heart failure and heart regulation mechanisms. The purpose of the present study was to demonstrate that flow velocity can be quantified in the coronary arteries of the isolated rat heart with high-resolution phase co...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.10563
更新日期:2003-09-01 00:00:00
abstract:PURPOSE:To develop and evaluate a novel 2D phase-unwrapping method that works robustly in the presence of severe noise, rapid phase changes, and disconnected regions. THEORY AND METHODS:The MR phase map usually varies rapidly in regions adjacent to wraps. In contrast, the phasors can vary slowly, especially in regions...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.26647
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:Magnetic resonance (MR) cholangiopancreatography (MRCP) is an established specialist method for imaging the upper abdomen and biliary/pancreatic ducts. Due to limitations of either MR image contrast or low through-plane resolution, patients may require further evaluation with contrast-enhanced computed tomograp...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.27852
更新日期:2019-11-01 00:00:00
abstract::Signal-to-noise ratio (SNR) is a major challenge to sodium magnetic resonance imaging. Phased array coils have been shown significantly improving SNR in proton imaging over volume coils. This study investigates SNR advantage of a 15-channel array head coil (birdcage volume coil for transmit/receive and 15-channel arra...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24192
更新日期:2012-12-01 00:00:00
abstract::Spatially resolved spectroscopy (SPARS) is a method for obtaining high-resolution NMR spectra of well-defined volumes of human tissues in situ. This method was combined with the Carr-Purcell-Meiboom-Gill multiple-echo sequence for T2, and with inversion recovery for T1 relaxation measurements. Relaxation times obtaine...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910040504
更新日期:1987-05-01 00:00:00