NMR relaxometric investigation on human methemoglobin and fluoromethemoglobin. An improved quantitative in vitro assay of human methemoglobin.

Abstract:

:Longitudinal water proton relaxation rates of methemoglobin solutions show a strong dependence on temperature and pH. The increase of the relaxation rates with temperature is associated with shortened exchange lifetime of the coordinated water molecule. An accurate measurement of the relaxation rate of methemoglobin solutions thus requires careful control of the experimental temperature. This observation prompted the authors to look for an improved version of the relaxometric in vitro determination of methemoglobin. The method is based on transforming methemoglobin into the corresponding fluoromethemoglobin derivative, which shows both a higher relaxivity and a negligible dependence on temperature. The proposed method has been found to be in good agreement with data from spectrophotometric assays.

journal_name

Magn Reson Med

authors

Aime S,Fasano M,Paoletti S,Arnelli A,Ascenzi P

doi

10.1002/mrm.1910330613

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

827-31

issue

6

eissn

0740-3194

issn

1522-2594

journal_volume

33

pub_type

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