Abstract：
:Photosensitizers belonging to the boron-dipyrromethenes (BODIPYs) class were recently found endowed with good efficacy in the antibacterial photodynamic therapy (aPDT) against both Gram-positive and Gram-negative bacteria. In this paper, we report on the remarkable adjuvant effect exerted in this respect by linear polyamidoamines (PAAs), a family of moderately basic polymers obtained by Michael-type polyaddition of amines to bisacrylamides. Three different PAAs (AGMA1, BP-AGMA, and BP-DMEDA) were studied, testing for each two different molecular weight samples (8000 and 24000 Da). At nontoxic concentrations (1 or 10 µg mL-1) all PAAs remarkably improved the killing efficacy of BODIPY upon irradiation with a green LED device (range: from 480 to 580 nm with λmax = 525 nm) up to an energy rate of 16.6 J cm-2. A 6-7 log unit decrease in bacteria survival was observed with concentrations of BODIPY of 1.0 and 0.1 µM in the case of Escherichia coli and Staphylococcus aureus, respectively. The one-way analysis of variance (ANOVA) was used to evaluate the statistical significance of different treatments (n ≥ 3). Thus, the PAA-photosensitizer combination warrants potentially as a new, effective, and mild method of killing bacteria. Moreover, the antibacterial treatment here reported might be successfully applied to defeat the bacterial resistance often encountered with many antibacterial drugs owing to the double action of this two-component treatment.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Caruso E,Ferrara S,Ferruti P,Manfredi A,Ranucci E,Orlandi VTdoi
10.1007/s10103-017-2345-0subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1401-1407issue
6eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-017-2345-0journal_volume
33pub_type
杂志文章abstract：:The aim of this study was to quantitatively evaluate the wettability of dentin after Yb:KYW thin-disk femtosecond-pulsed laser ablation by measuring the contact angle. Different laser parameters were used including different fluences (F), scanning speeds, and scanning line spacings. Crowns of 15 extracted human teeth ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-014-1655-8
更新日期：2015-08-01 00:00:00
abstract：:The biodistribution and pharmacokinetics of meta-tetra(hydroxyphenyl)chlorin (mTHPC)(1) have been documented in humans, rats, dogs and rabbits. It has been demonstrated to be an effective photodynamic therapy agent for treatment of squamous cell carcinoma. Squamous cell carcinoma is a common feline neoplasm, causing s...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s101030200014
更新日期：2002-01-01 00:00:00
abstract：INTRODUCTION:In-transit cutaneous malignant melanoma metastases remains a difficult and distressing disease. It is a sequela to the primary lesion represented by melanoma deposits between the site of excision and the draining lymph nodes. We present our experience of carbon dioxide ablation of in-transit cutaneous mali...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0580-0
更新日期：2009-05-01 00:00:00
abstract：:The objective of this study was to evaluate the safety and efficacy of flexible ureteroscopy (FURS) and holmium:YAG laser lithotripsy for the treatment of upper urinary tract stones in patients on active oral anticoagulants. The records of 1081 patients who underwent flexible ureteroscopic holmium:YAG (Ho:YAG) laser l...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-017-2289-4
更新日期：2017-09-01 00:00:00
abstract：:The current study evaluates whether the addition of low-level laser therapy into standard conventional physical therapy in elderly with bilateral symptomatic tri-compartmental knee arthritis can successfully postpone the need for joint replacement surgery. A prospective randomized cohort study of 100 consecutive unsel...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-015-1814-6
更新日期：2015-12-01 00:00:00
abstract：:In periodontal therapy, the use of low-level diode lasers has recently been considered to improve wound healing of the gingival tissue. However, its effects on human gingival epithelial cells (HGECs) remain unknown. The aim of the present study was to examine whether high-frequency low-level diode laser irradiation st...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-013-1292-7
更新日期：2014-07-01 00:00:00
abstract：:The effects of He-Ne laser irradiation on erythrocyte hemolysis were studied. Erythrocytes are prone to be easily damaged with hemolysis under laser irradiation. It was found that the power density (PD) of laser irradiation, but not the total irradiation power, is the crucial parameter for the hemolysis. Under irradia...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-004-0320-z
更新日期：2004-01-01 00:00:00
abstract：:The development of imaging technique to visualize and quantify the structural alteration of the spinal cord injury (SCI) may lead to better understanding and treatments of the injuries. In this work, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) was t...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-018-2630-6
更新日期：2019-04-01 00:00:00
abstract：:This study was designed to determine the laser dose for the stimulation, zero-bioactivation, and inhibition of normal and neoplastic cells in vitro. The medical use of laser biomodulation has been occurring for decades in the area of tissue healing and inflammatory conditions. The potential to modulate the regeneratio...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-1040-9
更新日期：2012-09-01 00:00:00
abstract：:In spinal cord injury (SCI), inflammation is a major mediator of damage and loss of function and is regulated primarily by the bone marrow-derived macrophages (BMDMs). Photobiomodulation (PBM) or low-level light stimulation is known to have anti-inflammatory effects and has previously been used in the treatment of SCI...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-019-02941-y
更新日期：2020-09-01 00:00:00
abstract：:Our objective was to assess the efficacy and safety of combined 10.6 microm and 650 nm laser irradiation on patients with knee osteoarthritis (OA). Forty patients with OA were randomly allocated to an active laser group or to a placebo laser group (20 per group). They either received active or sham laser treatment at ...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-007-0536-9
更新日期：2009-03-01 00:00:00
abstract：:Corrosiveness of enamel surfaces of Smartbleach, Opus White, Opalescense Xtra Boost and a gel containing titanium dioxide (TiO(2)) particles, activated either by a frequency doubled neodymium: yttrium-aluminum-garnet (Nd:YAG) laser (532 nm) or a diode laser (810 nm) was evaluated by environmental scanning electron mic...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0567-x
更新日期：2009-05-01 00:00:00
abstract：:The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-018-2685-4
更新日期：2019-07-01 00:00:00
abstract：:Previous in vivo and in vitro studies have reported that low-level diode laser therapy induces a biostimulatory effect, such as cell proliferation. The aim of the present study was to evaluate whether the laser irradiation of osteoblast-like cells (MG-63) can modify alkaline phosphatase activity (ALP), antigenic profi...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-014-1557-9
更新日期：2014-07-01 00:00:00
abstract：:A two-dimensional model including the effects of dynamic changes in the physical properties on tissue temperature and damage was developed to describe laser energy transport, heat transfer, and damage accumulation during laser-induced interstitial thermotherapy (LITT). The Monte Carlo method was used to simulate photo...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-004-0324-8
更新日期：2005-01-01 00:00:00
abstract：:In 30 patients with periodontitis, a total of 278 teeth exhibiting bleeding on probing, subgingival calculus, and a probing depth between 3-6 mm were examined. For each participant, two treatment types were alternatively applied on the contralateral quadrants: scaling and root planing (SRP) as control, and SRP followe...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-010-0799-4
更新日期：2011-07-01 00:00:00
abstract：:This study aimed to analyze the effects of light-emitting diode (LED) therapy on sternotomy pain and healing in patients who underwent coronary artery bypass grafting (CABG). The patients were followed for 6 months after the surgery to determine their dehiscence. This study was conducted with 90 volunteers who electiv...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-013-1503-2
更新日期：2014-05-01 00:00:00
abstract：:Low-level laser therapy (LLLT), widely used in physiotherapy, has been known to enhance wound healing and stimulate cell proliferation, including fibroblast and endothelial cells. Applying LLLT can increase cell proliferation in many kinds of cells including fibroblasts and endothelial cells. However, the protective m...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-017-2364-x
更新日期：2018-02-01 00:00:00
abstract：:Several studies suggest a biomodulatory influence of low-level laser irradiation in the inflammatory and reparative processes of dental tissues. The aim of this study was to investigate the stimulatory effect of 808-nm laser irradiation on root development of rat molars and also to evaluate the histological reaction o...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-0935-9
更新日期：2012-05-01 00:00:00
abstract：:Patients with pain avoid movements, leading to a gradual impairment of their physical condition and functionality. In this context, the use of ultrasound (US) and low-level laser therapy (LLLT) show promising results for nonpharmacological and noninvasive treatment. The aim of this study was evaluated the synergistic ...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-014-1659-4
更新日期：2015-01-01 00:00:00
abstract：:Preclinical studies have shown that photodynamic therapy (PDT) enhances immune responses. To examine the role of the direct effects of PDT in liver cancer with regard to enhancement of the antitumor response, we injected PDT-generated H22 liver cancer cell lysate (as a tumor vaccine) intradermally into Kunming mice. I...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0609-4
更新日期：2009-07-01 00:00:00
abstract：:Photobiomodulation (PBM) has been shown to improve wound healing by promoting mesenchymal stem cell migration and proliferation. However, it remains unknown whether an 808-nm diode laser can influence human gingival mesenchymal stem cells (HGMSCs), and which dose this works well. In the present study, it was found tha...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-020-03040-z
更新日期：2020-10-01 00:00:00
abstract：:Zirconia has been regarded as a promising material for dental implants, and Nd:YAG laser treatment has been proposed as a potential strategy to improve its bioactivity. The main aim of the present study was to evaluate the in vitro behavior of human fetal osteoblasts in contact with laser-textured zirconia implant sur...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-020-03066-3
更新日期：2020-12-01 00:00:00
abstract：:Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and i...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-014-1629-x
更新日期：2015-08-01 00:00:00
abstract：:Liquid-assisted hard tissue ablation by infrared lasers has extensive clinical application. However, detailed studies are still needed to explore the underlying mechanism. In the present study, the dynamic process of bubble evolution induced by Ho:YAG laser under water without and with bone tissue at different thickne...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-016-1931-x
更新日期：2016-07-01 00:00:00
abstract：:Bisphosphonate-related osteonecrosis of the jaw is a well-known potential side effect of long-term bisphosphonate therapy; the primary objective of the treatment should be to improve patient quality of life through pain and infection management, to prevent the development of new lesions, and to slow disease progressio...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
doi：10.1007/s10103-016-1929-4
更新日期：2016-08-01 00:00:00
abstract：:Acne vulgaris is one of the most common dermatologic disorders encountered in everyday practice. Treatment options for this often psychologically scarring disease are numerous and, for many individuals, provide relief from the disorder. However, factors such as antibiotic resistance and slow onset of action from many ...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
doi：10.1007/s10103-006-0420-z
更新日期：2007-06-01 00:00:00
abstract：:Acute lung injury (ALI) is defined as respiratory failure syndrome, in which the pathogenesis could occur from sepsis making it a life-threatening disease by uncontrolled hyperinflammatory responses. A possible treatment for ALI is the use of low-power infrared lasers (LPIL), whose therapeutical effects depend on wave...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-018-2656-9
更新日期：2019-02-01 00:00:00
abstract：:Induction of matrix synthesis by low-level laser has been demonstrated extensively. However, the question of dose- or power intensity-dependency is under-investigated. To address this issue we chose human osteoblast cell cultures and measured their alkaline phosphatase (ALP) activity after laser irradiation. The cell ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-007-0511-5
更新日期：2008-10-01 00:00:00
abstract：:From the very first reports describing the method of action of phototherapy, the effects have been considered to be the result of photochemical and photophysical interactions between the absorbed photons and tissue and not related to secondary changes in tissue or skin temperature. However, thermal effects have been r...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-015-1755-0
更新日期：2015-07-01 00:00:00
