Abstract：
:Low-level laser therapy (LLLT), widely used in physiotherapy, has been known to enhance wound healing and stimulate cell proliferation, including fibroblast and endothelial cells. Applying LLLT can increase cell proliferation in many kinds of cells including fibroblasts and endothelial cells. However, the protective mechanisms of LLLT on endothelial apoptosis remain unclear. We hypothesized LLLT can protect endothelial cells from inflammation-induced apoptosis. Human endothelial cell line, EA.hy926 cells, and TNF-α/cycloheximide (TNF/CHX) were used to explore the protective effects of LLLT (660 nm) on inflammation-induced endothelial apoptosis. Cell viability, apoptosis, caspase-3/7/8/9 activity, MAPKs signaling, NF-κB activity, and inducible/endothelial nitric oxide synthase (iNOS/eNOS) expression were measured. Our results showed that LLLT increased EA.hy926 cell proliferation, attenuated the TNF/CHX-induced apoptosis, and reduced the TNF/CHX-mediated caspase-3/7/8/9 activation. In addition, LLLT increased ERK MAPK phosphorylation and suppressed the TNF/CHX-increased p38 MAPK, JNK, IKK phosphorylation, NF-κB translocation, and iNOS expression. The caspases-3 cleavage and cell death were not increased in cells treating with ERK inhibitor U0126, which implicated that ERK is not to be responsible for the protective effects of LLLT. After treating with p38 mitogen-activated protein kinase (MAPK) activator, the protection of LLLT in cell apoptosis was no longer existed, showing that LLLT protected the endothelial cells by suppressing p38 MAPK signaling. Our results provide a new insight into the possible molecular mechanisms in which LLLT protects against inflammatory-induced endothelial dysfunction.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Chu YH,Chen SY,Hsieh YL,Teng YH,Cheng YJdoi
10.1007/s10103-017-2364-xsubject
Has Abstractpub_date
2018-02-01 00:00:00pages
279-286issue
2eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-017-2364-xjournal_volume
33pub_type
杂志文章abstract：:The aim of this study was to investigate the effect of different working modes (pulsed and micropulsed) and power settings of a standardized 980-nm diode laser on collateral thermal soft-tissue damage. A total of 108 bovine liver samples were cut with a diode laser at various settings in pulsed and micropulsed mode an...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-1007-x
更新日期：2012-09-01 00:00:00
abstract：:Our previous in vitro study has shown that toluidine blue (TB)-mediated lethal photosensitization of periodontal pathogens (PPs) from periodontal patients is possible. The purpose of this study was to investigate whether TB-mediated photosensitization exerted damaging effects on periodontal tissues in mice. Twenty-fou...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-007-0513-3
更新日期：2009-03-01 00:00:00
abstract：:It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is controversial. The purpose of this meta-analysis was to evaluate the effects of LAH on pregnancy outcomes of cryopreserve...
journal_title：Lasers in medical science
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s10103-017-2372-x
更新日期：2018-04-01 00:00:00
abstract：:Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. B...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-0945-7
更新日期：2012-01-01 00:00:00
abstract：:The erbium:YSGG and erbium:YAG lasers are used for tissue ablation in dermatology, dentistry and ophthalmology. The purpose of this study was to compare germanium oxide and sapphire optical fibres for transmission of sufficient Q-switched erbium laser pulse energies for potential use in both soft and hard tissue ablat...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-004-0316-8
更新日期：2004-01-01 00:00:00
abstract：:The post-stimulation response of neural activities plays an important role to evaluate the effectiveness and safety of neural modulation techniques. Previous studies have established the capability of infrared neural modulation (INM) on neural firing regulation in the central nervous system (CNS); however, the dynamic...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-019-02826-0
更新日期：2020-03-01 00:00:00
abstract：:The purpose of this study was to evaluate the thickness and qualitative characteristics of the hybrid layer after two cavity preparation methods, using Er:YAG laser in QSP mode and conventional carbide burs. Additionally, two different adhesive techniques were investigated using etch-and-rinse and self-etch adhesive s...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-018-2575-9
更新日期：2019-02-01 00:00:00
abstract：:Oxygen consumption at the targeted site has a significant effect on dosimetry in photodynamic therapy (PDT). However, oxygen consumption in PDT using a pulsed laser as a light source has not been clarified. We therefore investigated the dependence of cytotoxicity on the oxygen consumption and the photosensitizer photo...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-004-0288-8
更新日期：2004-01-01 00:00:00
abstract：:This study aimed to investigate the influence of low-power gallium-aluminium-arsenide (GaAlAs) laser [830 nm, continuous wave (CW), 40 mW and fluence 4 J/cm(2)] on the healing of surgically created bone defects in rats treated with bioactive glass graft material. Surgical bone defects were created in the mandibles of ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0590-y
更新日期：2009-07-01 00:00:00
abstract：:Low-level red laser (LLRL)-tissue interactions have a wide range of medical applications and are garnering increased attention. Although the positive effects of low-level laser therapy (LLLT) have frequently been reported and enhanced collagen accumulation has been identified as one of the most important mechanisms in...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-015-1823-5
更新日期：2015-12-01 00:00:00
abstract：:The aim of this in vitro study was to evaluate some parameters of dental etching when irradiated with an erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser. One-hundred sound human third molars were selected and randomly distributed into ten groups (n = 10). The class V cavities of group 1 (control) w...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0623-6
更新日期：2010-05-01 00:00:00
abstract：:We evaluate the safety, efficacy, and oncological outcomes of early palliative photoselective vaporization of the prostate (PVP) by GreenLight high-performance system (HPS) 120-W laser in patients with acute urinary retention (AUR) induced by advanced prostate cancer (PCa). A total of 39 advanced PCa patients with AUR...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-012-1252-7
更新日期：2013-09-01 00:00:00
abstract：:In this study we compared the microleakage of conventional glass ionomer cement (GIC) restorations following the use of different methods of root caries removal. In vitro root caries were induced in 75 human root dentin samples that were divided in five groups of 15 each according to the method used for caries removal...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-010-0840-7
更新日期：2012-01-01 00:00:00
abstract：:Acne vulgaris is one of the most common dermatologic disorders encountered in everyday practice. Treatment options for this often psychologically scarring disease are numerous and, for many individuals, provide relief from the disorder. However, factors such as antibiotic resistance and slow onset of action from many ...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
doi：10.1007/s10103-006-0420-z
更新日期：2007-06-01 00:00:00
abstract：:The aim of this in vitro study was to assess the effect of Er:YAG laser surface treatment on the tensile bond strength of a sealant in permanent teeth. A total of 30 sound third molars were selected and embedded in cold-cure acrylic resin. The enamel surfaces were flattened by a grinding. The teeth were randomly divid...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-010-0869-7
更新日期：2012-03-01 00:00:00
abstract：:Laser excision has been used with increasing success to treat oral leukoplakia. The aim of the present study was to evaluate whether clinical healing of a leukoplakia after laser surgery is associated with a normal functional status of the new epithelium and whether pathological alterations in these parameters are rel...
journal_title：Lasers in medical science
pub_type： 临床试验,杂志文章
doi：10.1007/s10103-011-0941-y
更新日期：2012-01-01 00:00:00
abstract：:Selecting the most representative site for biopsy is crucial in establishing a definitive diagnosis of oral epithelial dysplasia. The current process involves clinical examination that can be subjective and prone to sampling errors. The aim of this study was therefore to investigate the use of optical coherence tomogr...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-0975-1
更新日期：2012-07-01 00:00:00
abstract：:We report the results of endoscopic laser therapy on 59 patients with upper gastrointestinal vascular ectasia. After 300 sessions, complications were two nonbleeding and three bleeding ulcers, treated successfully with endoscopic therapy. After treatment, blood transfusions were abolished in 61% and reduced in 22% of ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-006-0389-7
更新日期：2006-09-01 00:00:00
abstract：:Facial vitiligo has considerable psychological and emotional impacts. Though topical corticosteroids are the mainstay treatment, adverse reactions are a concern for prolonged use. Additionally, 308-nm excimer lamps have been widely used in vitiligo. This study aimed to assess the effectiveness of a combined 308-nm exc...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-020-03048-5
更新日期：2020-12-01 00:00:00
abstract：:The aim of the study was to investigate the effect of low-level 809 nm laser irradiation on the proliferation rate of human larynx carcinoma cells in vitro. Epithelial tumor cells were obtained from a laryngeal carcinoma and cultured under standard conditions. For laser treatment the cells were spread on 96-well tissu...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-003-0265-7
更新日期：2003-01-01 00:00:00
abstract：:Infrared laser therapy is used for skeletal muscle repair based on its biostimulative effect on satellite cells. However, shortening of telomere length limits regenerative potential in satellite cells, which occurs after each cell division cycle. Also, laser therapy could be more effective on non-physiologic tissues. ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-016-1956-1
更新日期：2016-08-01 00:00:00
abstract：:The purpose of this study is to evaluate microtensile bond strength (μTBS) of an etch-and-rinse adhesive to enamel and dentin after treatment with Er:YAG laser using different pulse durations. Extracted human molars were flattened to obtain enamel or dentin surfaces. The enamel specimens (E) were divided into nine gro...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-010-0830-9
更新日期：2012-01-01 00:00:00
abstract：:Low-intensity laser therapy (LILT) has been considered as a treatment modality in diabetic distal symmetric polyneuropathy (DSP). The aim of this study is to determine the effectiveness of LILT on DSP. We examined 107 subjects with type 2 diabetes for detection of DSP using the Michigan Neuropathy Screening Instrument...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-0977-z
更新日期：2011-11-01 00:00:00
abstract：:A nevus is described as a benign, often pigmented, skin growth that can be congenital or acquired. Different types of nevi are located at different depths within the skin. Traditional treatments for nevi include topical treatments, dermabrasion, and excision, which are generally ineffective, invasive, and often result...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
doi：10.1007/s10103-014-1697-y
更新日期：2015-09-01 00:00:00
abstract：:In root canal therapy, irrigating solutions are essential to assist in debridement and disinfection. Their spread and action is often restricted by canal anatomy, requiring some form of activation. Lasers have been shown to be promising tools for this purpose (laser-activated irrigation (LAI)). For LAI to be effective...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-013-1307-4
更新日期：2014-09-01 00:00:00
abstract：:A growing number of laboratory and clinical studies over the past 10 years have shown that low-level laser stimulation (633 or 670 nm) at extremely low power densities (about 0.15 mW/cm(2)), when administered through a particular emission mode, is capable of eliciting significant biological effects. Studies on cell cu...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
doi：10.1007/s10103-010-0837-2
更新日期：2011-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the acid resistance of erbium:yttrium-aluminum-garnet (Er:YAG) lased sub-surface dentin using a scanning electron microscope (SEM). Dentin disks were exposed to a single pulse of Er:YAG laser irradiation at 80 mJ/pulse under water spray, with the contact sapphire tip vertical ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-008-0553-3
更新日期：2009-07-01 00:00:00
abstract：:The aim of this study was to verify the maximum number of repetitions, fatigue index, blood lactate concentration ([Lac]), and cardiac autonomic responses after LED irradiation (LEDI) in the ipsilateral and contralateral limb. Twelve male subjects (22.0 ± 3.86 years; weight 82.94 ± 12.58 kg; height 1.77 ± 0.05 m), phy...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-017-2391-7
更新日期：2018-02-01 00:00:00
abstract：:The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295-330 g) were bilaterally ovariectomized and divided into four groups (n ...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-012-1251-8
更新日期：2013-11-01 00:00:00
abstract：:In order to design and manufacture a reliable biomedical sensor, sensitive tests have to be performed. This is of superlative importance as it has to be used for the general public. Keeping this into account, the test mechanism of such an extremely sensitive sensor is being done that involves exhaustive protocols rega...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-019-02945-8
更新日期：2020-09-01 00:00:00
