Abstract:
:Selecting the most representative site for biopsy is crucial in establishing a definitive diagnosis of oral epithelial dysplasia. The current process involves clinical examination that can be subjective and prone to sampling errors. The aim of this study was therefore to investigate the use of optical coherence tomography (OCT) for differentiation of normal and dysplastic oral epithelial samples, with a view to developing an objective and reproducible approach for biopsy site selection. Biopsy samples from patients with fibro-epithelial polyps (n = 13), mild dysplasia (n = 2), and moderate/severe dysplasia (n = 4) were scanned at 5-μm intervals using an OCT microscope and subsequently processed and stained with hematoxylin and eosin (H&E). Epithelial differentiation was measured from the rate of change (gradient) of the backscattered light intensity in the OCT signal as a function of depth. This parameter is directly related to the density of optical scattering from the cell nuclei. OCT images of normal oral epithelium showed a clear delineation of the mucosal layers observed in the matching histology. However, OCT images of oral dysplasia did not clearly identify the individual mucosal layers because of the increased density of abnormal cell nuclei, which impeded light penetration. Quantitative analysis on 2D-OCT and histology images differentiated dysplasia from normal control samples. Similar analysis on 3D-OCT datasets resulted in the reclassification of biopsy samples into the normal/mild and moderate/severe groups. Quantitative differentiation of normal and dysplastic lesions using OCT offers a non-invasive objective approach for localizing the most representative site to biopsy, particularly in oral lesions with similar clinical features.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Adegun OK,Tomlins PH,Hagi-Pavli E,McKenzie G,Piper K,Bader DL,Fortune Fdoi
10.1007/s10103-011-0975-1subject
Has Abstractpub_date
2012-07-01 00:00:00pages
795-804issue
4eissn
0268-8921issn
1435-604Xjournal_volume
27pub_type
杂志文章abstract::Skin represents an interface between internal and external environment; it protects human body by regulating the water loss and the maintenance of body temperature, defending against irritant and pathogen agents, and against physical, chemical, and UV damage. It provides to essential physiological functions, such as t...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-018-2635-1
更新日期:2019-04-01 00:00:00
abstract::Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. B...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0945-7
更新日期:2012-01-01 00:00:00
abstract::The objective of this study was to describe the dynamics of water heating carried out by continuous laser radiation with wavelengths 1.47, 1.55, and 1.94 μm with different types of fibers used for endovenous laser coagulation. The study was conducted in water using high-speed surveying of the heating process through t...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03184-y
更新日期:2020-11-07 00:00:00
abstract::The aims of this study were to evaluate the effects of Photogem®-mediated photosensitization on rat palatal mucosa and the biodistribution of the photosensitizer in this tissue. A solution of Photogem® (500 or 1000 mg/l) was applied to the palatal mucosa for 30 min and the exposure time to blue LED (460 nm) was 20 min...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0909-y
更新日期:2012-03-01 00:00:00
abstract::This study aimed to investigate the effects of administering photobiomodulation therapy (PBM) with bovine bone matrix on critical size defects in rats. Seventy-two adult male rats (albinus, Wistar), 90 days old, were used. Defect of 5 mm in diameter was made in their calvaria. The animals were divided into 4 groups: C...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03234-5
更新日期:2021-01-05 00:00:00
abstract::Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototyp...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-012-1057-8
更新日期:2013-02-01 00:00:00
abstract::Although some studies claim to the increase of composite resin adhesion to Er:YAG-laser-treated dentin, there are still no reports on the adhesion of composite resin cements to the irradiated surface. This in vitro study evaluated the tensile bond strength (TBS) of a composite resin cement to dentin treated with the E...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-006-0433-7
更新日期:2007-09-01 00:00:00
abstract::The aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12 weeks) were randomly distributed among control group (n =...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03155-3
更新日期:2020-10-10 00:00:00
abstract::Previous studies have been suggested that photodynamic therapy (PDT) can be used as an adjuvant treatment for denture stomatitis. In this study, we evaluated the effects of multiple sessions of PDT on Candida glabrata biofilms in specimens of polymerized acrylic resin formed after 5 days. Subsequently, four applicatio...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2147-4
更新日期:2017-04-01 00:00:00
abstract::The effect of low-level laser therapy (LLLT) on the cardiovascular system is not fully established. Since the endothelium is an important endocrine element, establishing the mechanisms of LLLT action is an important issue.The aim of the study was to evaluate the effect of transdermal LLLT on endothelial function.In th...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
doi:10.1007/s10103-016-1971-2
更新日期:2016-09-01 00:00:00
abstract::In 30 patients with periodontitis, a total of 278 teeth exhibiting bleeding on probing, subgingival calculus, and a probing depth between 3-6 mm were examined. For each participant, two treatment types were alternatively applied on the contralateral quadrants: scaling and root planing (SRP) as control, and SRP followe...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-010-0799-4
更新日期:2011-07-01 00:00:00
abstract::Central serous chorioretinopathy (CSC) is a disease characterized by a well-defined serous detachment of the neurosensory retina. Therapeutic options in chronic cases are limited, and side effects can result in a limited response. The aim of this article is to assess the efficacy of subthreshold micropulse laser thera...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03129-5
更新日期:2020-08-19 00:00:00
abstract::This retrospective case-control study aimed to compare 30 versus 40 W power of CO2 laser for the therapy of genitourinary syndrome of menopause (GSM). Postmenopausal women with severe intensity of dyspareunia and dryness were eligible to be included in this study. Primary outcomes were dyspareunia and dryness. Seconda...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2293-8
更新日期:2017-11-01 00:00:00
abstract::The aim of this study was to compare the rate of tooth displacement, quantity of root resorption, and alveolar bone changes in five groups: corticopuncture (CP), low-level laser therapy (LLLT), CP combined with LLLT (CP + LLLT), control (C), and negative control (NC). A total of 60 half-maxilla from 30 male Wistar rat...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2421-5
更新日期:2018-05-01 00:00:00
abstract::Osteoarthritis (OA) triggers increased levels of inflammatory markers, including prostaglandin (PG) E2 and proinflammatory cytokines. The elevation of cytokine levels is closely associated with increased articular tissue degeneration. Thus, the use of combination therapies may presumably be able to enhance the effects...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-016-2091-8
更新日期:2017-01-01 00:00:00
abstract::Knee osteoarthritis (KOA) is a common degenerative disease in which several treatments and treatment associations have been investigated. This review analyzed the efficacy of the association of photobiomodulation therapy (PBMT) and exercises for people with KOA in randomized controlled clinical trials. PubMed, Scopus,...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
doi:10.1007/s10103-020-03223-8
更新日期:2021-01-03 00:00:00
abstract::The aim of this study is to evaluate the effectiveness of the low-level laser therapy (LLLT) in the control of pain, swelling, and trismus associated with surgical removal of impacted lower third molars. Thirty patients were randomized into two treatment groups, each with 15 patients-group test (LLLT) and a group cont...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-012-1174-4
更新日期:2013-05-01 00:00:00
abstract::Quantitative characterization of skin collagen on photo-thermal response and its regeneration process is an important but difficult task. In this study, morphology and spectrum characteristics of collagen during photo-thermal response and its light-induced remodeling process were obtained by second-harmonic generation...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1296-3
更新日期:2014-01-01 00:00:00
abstract::The use of the CO(2) laser in the management of oral dysplastic lesions has become a more common practice. Very few studies have evaluated recurrence, residual disease malignant transformation, and overall outcome in patients undergoing such a procedure. In this prospective study, a total of 123 oral dysplastic lesion...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
doi:10.1007/s10103-011-0916-z
更新日期:2012-01-01 00:00:00
abstract::Delivery of intradermally focused nanosecond laser pulses with small energy as an alternate technique applicable to clinical procedures in dermatological and plastic surgery is an area of relatively new interest with multiple potential applications. We assessed this approach on common tattoo pigments in dermis in an i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030200025
更新日期:2002-01-01 00:00:00
abstract::We report results on the use of organically modified silica nanoparticles (SiNp) as a vehicle for the delivery of curcumin in human oral cancer cells for improvement of uptake and phototoxicity. Nanoformulated drug (curcumin-SiNp complex) was prepared by postloading curcumin in SiNp, and the complex was soluble in aqu...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1357-7
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:Acne scarring represents a common problem that negatively impacts patients' quality of life. Different types of treatments are currently available for this dermatological condition. This study evaluates the efficacy of a new 675-nm laser source system on acne scars with the use of established parameters that gu...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03063-6
更新日期:2021-02-01 00:00:00
abstract::. The goal of this study is an in vitro evaluation of thermal side-effects by the application of short sub- micro s CO(2) laser pulses in combination with an air-water spray on different types of bone tissue. A mechanically Q-switched CO(2) laser delivered 300 ns pulses at 9.6 micro m wavelength, which were focused do...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030200038
更新日期:2002-01-01 00:00:00
abstract::The aim of the present study was to investigate and compare the effects of high intensity laser therapy (HILT) and low level laser therapy (LLLT) on the treatment of patients with Bell's palsy. Forty-eight patients participated in and completed this study. The mean age was 43 ± 9.8 years. They were randomly assigned i...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-013-1352-z
更新日期:2014-01-01 00:00:00
abstract::This study aimed to compare the effects of photobiomodulation (PBM) in different energy densities and irradiances on maintaining cell viability, and proliferation of pulp fibroblasts from human primary teeth (HPF) were cultured in DMEM and used between the fourth and eighth passages. Then, HPF were irradiated with the...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2301-z
更新日期:2017-09-01 00:00:00
abstract::Retinal development involves a complex coordination of events that may be affected by premature delivery, leading to retinopathy of prematurity (ROP), a potential blinding childhood disease. The avascular retina drives further disease progression, with laser ablation reducing unfavorable anatomic outcomes as well as m...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
doi:10.1007/s10103-011-1021-z
更新日期:2013-02-01 00:00:00
abstract::The biodistribution and pharmacokinetics of meta-tetra(hydroxyphenyl)chlorin (mTHPC)(1) have been documented in humans, rats, dogs and rabbits. It has been demonstrated to be an effective photodynamic therapy agent for treatment of squamous cell carcinoma. Squamous cell carcinoma is a common feline neoplasm, causing s...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030200014
更新日期:2002-01-01 00:00:00
abstract::The aim of the present study was to evaluate the antimicrobial effect of antimicrobial photodynamic therapy (aPDT) in alveolar treatment of areas with induced periodontitis. Thirty male Wistar rats were subjected to ligature-induced periodontal disease (PD) in the first left inferior molars, while the right side molar...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1400-8
更新日期:2015-02-01 00:00:00
abstract::Gingival melanin hyperpigmentation is an esthetic concern for many individuals. In this study, we compared the standard surgical removal method with two different Er,Cr:YSGG laser settings in order to find the best treatment method. In 33 dental arches, the following three treatment groups were comparatively evaluated...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-018-2501-1
更新日期:2018-11-01 00:00:00
abstract::In an in vitro study, the effect of light polarization on the efficiency of 5-aminolaevulinic acid (ALA) photodynamic therapy (PDT) of basal cell carcinoma (BCC) was investigated. Three states of light polarization (non-polarized, linearly polarized, and circularly polarized) were considered. Cells were exposed to gre...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2369-5
更新日期:2018-02-01 00:00:00