Abstract：
:Oxygen consumption at the targeted site has a significant effect on dosimetry in photodynamic therapy (PDT). However, oxygen consumption in PDT using a pulsed laser as a light source has not been clarified. We therefore investigated the dependence of cytotoxicity on the oxygen consumption and the photosensitizer photobleaching of PDT using a pulsed laser by comparing with that using a continuous wave (CW) laser. Mouse renal carcinoma cells (Renca) were incubated with a second-generation photosensitizer, PAD-S31. The cells were then irradiated with either a 670-nm nanosecond pulsed light from the 3rd harmonics of a Nd:YAG laser-pumped optical parametric oscillator with a peak fluence rate of approximately 1 MW/cm(2) at 30 Hz or a 670-nm CW diode laser with a total light dose of 40 J/cm(2). Regardless of laser source, cytotoxic effects exhibited cumulative dose responses to the photosensitizer ranging from 12 to 96 microg/ml. However, cytotoxic effect of PDT using the pulsed light was significantly less than that using the CW light with the photosensitizer concentrations of 24 and 48 microg/ml under identical fluence rates. During PDT, the cells exposed to the pulsed light consumed oxygen more slowly, resulting in a lower amount of oxygen consumption when compared with PDT using CW light. In accordance with oxygen consumption, the pulsed light induced significantly less photobleaching of the photosensitizer than the CW light did. These results indicate that the efficiency of PDT using pulsed light is less when compared with CW light, probably being related to suppressed oxygen consumption during the pulsed light irradiation.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Kawauchi S,Morimoto Y,Sato S,Arai T,Seguchi K,Asanuma H,Kikuchi Mdoi
10.1007/s10103-004-0288-8subject
Has Abstractpub_date
2004-01-01 00:00:00pages
179-83issue
4eissn
0268-8921issn
1435-604Xjournal_volume
18pub_type
杂志文章abstract：:Neurofibromatosis type 1 (NF-1) is an autosomal dominant disorder characterized by skin tumours derived from peripheral nerves. It is a clinically diagnosed disorder of a mainly cosmetic concern. There are different excision modalities for treatment of cutaneous neurofibromas; however, none is considered to be univers...
journal_title：Lasers in medical science
pub_type： 杂志文章
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journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-017-2323-6
更新日期：2018-01-01 00:00:00
abstract：:Acupuncture is a form of traditional Chinese medicine that has developed over thousands of years. We studied the effects of laser puncture, needle acupuncture, and light stimulation on cerebral blood flow in 15 healthy volunteers (mean age 25.0±1.9 years, 5 female, 10 male) with non-invasive transcranial Doppler sonog...
journal_title：Lasers in medical science
pub_type： 杂志文章
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abstract：:To determine the relation between retinal microstructural changes and the response to 577-nm subthreshold micropulse laser (SML) treatment in chronic central serous chorioretinopathy (cCSC). This retrospective study included 39 eyes of 39 patients with cCSC, treated with the 577-nm SML. The eyes were evaluated in thre...
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pub_type： 杂志文章
doi：10.1007/s10103-020-03225-6
更新日期：2021-01-07 00:00:00
abstract：:Low-level laser irradiation (LLLI) and recombinant human bone morphogenetic protein type 2 (rhBMP-2) have been used to stimulate bone formation. LLLI stimulates proliferation of osteoblast precursor cells and cell differentiation and rhBMP-2 recruits osteoprogenitor cells to the bone healing area. This in vivo study e...
journal_title：Lasers in medical science
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更新日期：2012-09-01 00:00:00
abstract：:A two-dimensional model including the effects of dynamic changes in the physical properties on tissue temperature and damage was developed to describe laser energy transport, heat transfer, and damage accumulation during laser-induced interstitial thermotherapy (LITT). The Monte Carlo method was used to simulate photo...
journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2005-01-01 00:00:00
abstract：:In this study, shear bond strength (SBS) of metal orthodontic brackets to porcelain following conditioning by Er:YAG (erbium-doped yttrium aluminum garnet) and Nd:YAG (neodymium-doped yttrium aluminum garnet) laser in comparison to conventional methods was evaluated. One hundred glazed porcelain discs with an upper ce...
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abstract：:Precise nerve localization is of major importance in both surgery and regional anesthesia. Optically based techniques can identify tissue through differences in optical properties, like absorption and scattering. The aim of this study was to evaluate the potential of optical spectroscopy (diffuse reflectance spectrosc...
journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
abstract：:The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly...
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pub_type： 杂志文章,随机对照试验
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更新日期：2013-05-01 00:00:00
abstract：:In order to design and manufacture a reliable biomedical sensor, sensitive tests have to be performed. This is of superlative importance as it has to be used for the general public. Keeping this into account, the test mechanism of such an extremely sensitive sensor is being done that involves exhaustive protocols rega...
journal_title：Lasers in medical science
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更新日期：2020-09-01 00:00:00
abstract：:The bond strength of resin composite to Er,Cr:YSGG laser-irradiated enamel and dentin has been evaluated in only a few studies. Therefore, we measured and compared the micro-shear bond strength of composite restorations to enamel and dentin using two different cavity-preparation tools and conditioning methods. One hun...
journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-011-0961-7
更新日期：2012-07-01 00:00:00
abstract：:Low-energy laser irradiation (Ga-Al-As semiconductor laser, output 50 mW) was applied to rat osteoclast precursor cells for 1, 3, 6, or 10 min at 24-h intervals during the culture period. The number of tartrate-resistant acid phosphatase positive multinucleate cells was increased by approximately 1.3-fold in the 3- an...
journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
abstract：:This study aimed to investigate the effects of administering photobiomodulation therapy (PBM) with bovine bone matrix on critical size defects in rats. Seventy-two adult male rats (albinus, Wistar), 90 days old, were used. Defect of 5 mm in diameter was made in their calvaria. The animals were divided into 4 groups: C...
journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2021-01-05 00:00:00
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pub_type： 临床试验,杂志文章
doi：10.1007/s10103-017-2259-x
更新日期：2017-08-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/s10103-013-1366-6
更新日期：2015-02-01 00:00:00
abstract：:The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295-330 g) were bilaterally ovariectomized and divided into four groups (n ...
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更新日期：2013-11-01 00:00:00
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journal_title：Lasers in medical science
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abstract：:This retrospective case-control study aimed to compare 30 versus 40 W power of CO2 laser for the therapy of genitourinary syndrome of menopause (GSM). Postmenopausal women with severe intensity of dyspareunia and dryness were eligible to be included in this study. Primary outcomes were dyspareunia and dryness. Seconda...
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更新日期：2019-07-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2010-05-01 00:00:00
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journal_title：Lasers in medical science
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更新日期：2009-01-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
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journal_title：Lasers in medical science
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更新日期：2010-01-01 00:00:00
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abstract：:Our objective was to assess the efficacy and safety of combined 10.6 microm and 650 nm laser irradiation on patients with knee osteoarthritis (OA). Forty patients with OA were randomly allocated to an active laser group or to a placebo laser group (20 per group). They either received active or sham laser treatment at ...
journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10103-007-0536-9
更新日期：2009-03-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
doi：10.1007/BF02765103
更新日期：1997-10-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
