Abstract:
:Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and investigated the microbial reduction produced when different light delivery methods are employed. Each light delivery method was evaluated in respect to the light distribution provided inside curved root canals. After conventional endodontic preparation, teeth were sterilized before canals were contaminated by a bioluminescent strain of C. albicans (CEC789). Methylene blue (90 μM) was introduced into the canals and then irradiated (λ = 660 nm, P = 100 mW, beam diameter = 2 mm) with laser tip either in contact with pulp chamber or within the canal using an optical diffuser fiber. Light distribution was evaluated by CCD camera, and microbial reduction was monitored through bioluminescence imaging. Our findings demonstrated that the bioluminescent C. albicans biofilm model had good reproducibility and uniformity. Light distribution in dental tissue was markedly dependent on the light delivery system, and this strategy was directly related to microbial destruction. Both light delivery systems performed significant fungal inactivation. However, when irradiation was performed with optical diffuser fiber, microbial burden reduction was nearly 100 times more effective. Bioluminescence is an interesting real-time analysis to endodontic C. albicans biofilm inactivation. APDT showed to be an effective way to inactivate C. albicans biofilms. Diffuser fibers provided optimized light distribution inside curved root canals and significantly increased APDT efficiency.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Sabino CP,Garcez AS,Núñez SC,Ribeiro MS,Hamblin MRdoi
10.1007/s10103-014-1629-xsubject
Has Abstractpub_date
2015-08-01 00:00:00pages
1657-65issue
6eissn
0268-8921issn
1435-604Xjournal_volume
30pub_type
杂志文章abstract::Transoral laser microsurgery (TLM) is an emerging technique for the management of laryngeal and other head and neck malignancies. It is increasingly being used in place of traditional open surgery because of lower morbidity and improved organ preservation. Since the surgery is performed from the inside working outward...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章,评审
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journal_title:Lasers in medical science
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pub_type: 临床试验,杂志文章
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章,meta分析,评审
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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journal_title:Lasers in medical science
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