Abstract:
:Acute lung injury (ALI) is defined as respiratory failure syndrome, in which the pathogenesis could occur from sepsis making it a life-threatening disease by uncontrolled hyperinflammatory responses. A possible treatment for ALI is the use of low-power infrared lasers (LPIL), whose therapeutical effects depend on wavelength, power, fluence, and emission mode. The evaluation mRNA levels of repair gene related to oxidative damage after exposure to LPIL could provide important information about the modulation of genes as treatment for ALI. Thus, the aim of this study was to evaluate the mRNA levels from OGG1, APEX1, ERCC2, and ERCC1 genes in lung tissue from Wistar rats affected by ALI and after exposure to LPIL (808 nm; 100 mW). Adult male Wistar rats (n = 30) were randomized into six groups (n = 5, for each group): control, 10 J/cm2 (2 J), 20 J/cm2 (5 J), ALI, ALI + LPIL 10 J/cm2 and ALI + LPIL 20 J/cm2. ALI was induced by intraperitoneal E. coli lipopolysaccharide injection (10 mg/kg). Lungs were removed, and samples were withdrawn for total RNA extraction, cDNA synthesis, and mRNA levels were evaluated by RT-qPCR. Data normality was verified by Kolmogorov-Smirnov, comparisons among groups were by Student's t test, Mann-Whitney test, one-way ANOVA, Kruskal-Wallis followed by post-tests. Data showed that OGG1 (0.39 ± 0.10), ERCC2 (0.67 ± 0.24), and ERCC1 (0.60 ± 0.19) mRNA levels are reduced in ALI group when compared with the control group (1.00 ± 0.07, 1.03 ± 0.25, 1.01 ± 0.16, respectively) and, after LPIL, mRNA relative levels from DNA repair genes are altered when compared to non-exposed ALI group. Our research shows that ALI alter mRNA levels from genes related to base and nucleotide excision repair genes, suggesting that DNA repair is part of cell response to sepsis, and that photobiomodulation could modulate the mRNA levels from these genes in lung tissue.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
da Silva Sergio LP,Thomé AMC,da Silva Neto Trajano LA,Vicentini SC,Teixeira AF,Mencalha AL,de Paoli F,de Souza da Fonseca Adoi
10.1007/s10103-018-2656-9subject
Has Abstractpub_date
2019-02-01 00:00:00pages
157-168issue
1eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-018-2656-9journal_volume
34pub_type
杂志文章abstract::Hyperthermia treatment can induce component changes on cell. This study explored the potential of Z-scan to improve accuracy in the identification of subtle differences in mouse colon cancer cell line CT26 during hyperthermia treatment. Twenty-one samples were subjected individually to treatment of hyperthermia at 41,...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-019-02759-8
更新日期:2019-10-01 00:00:00
abstract::A retrospective study was conducted to investigate the incidence of reported adverse responses after epilatory laser treatment to control the growth of facial hair in patients with polycystic ovary syndrome. Forty-eight patients received a median of 6 (range 2-11) treatments with an Aesculap ruby laser during a 704 da...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-007-0451-0
更新日期:2008-01-01 00:00:00
abstract::Delivery of intradermally focused nanosecond laser pulses with small energy as an alternate technique applicable to clinical procedures in dermatological and plastic surgery is an area of relatively new interest with multiple potential applications. We assessed this approach on common tattoo pigments in dermis in an i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030200025
更新日期:2002-01-01 00:00:00
abstract::We report the use of spectral domain polarization sensitive optical coherence tomography for ex-vivo imaging of human oral mandibular tissue samples. Our results show that compared to the changes observed in the epithelium thickness and the decay constant of A-scan intensity profile, a much larger degree of change was...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-016-2096-3
更新日期:2017-01-01 00:00:00
abstract::Down syndrome (DS) has characteristics that include mental retardation, a characteristic phenotype, congenital heart defects, immune disorders, and increased risk of periodontal disease (PD). Antimicrobial photodynamic therapy (aPDT) is the combined use of photosensitizers associated with low-level laser (LLL) and oxy...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-016-2020-x
更新日期:2016-12-01 00:00:00
abstract::Core-shell nanoparticles have unusual physical, chemical and biological properties. Until now, for the Ag and TiO2 combination, only Ag core and TiO2 shell nanoparticles have been practically demonstrated. In this investigation, novel TiO2@Ag core-shell (TiO2 core and Ag shell) nanoparticles were produced via ultrason...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-015-1855-x
更新日期:2016-02-01 00:00:00
abstract::It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is controversial. The purpose of this meta-analysis was to evaluate the effects of LAH on pregnancy outcomes of cryopreserve...
journal_title:Lasers in medical science
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s10103-017-2372-x
更新日期:2018-04-01 00:00:00
abstract::Most of the widely used scales to evaluate scars are subjective relying on clinical observations. There is a growing need to find out a noninvasive objective tool for this purpose. The study is aimed at evaluating the value of the high-resolution ultrasound in the assessment of the scars when compared with a clinical ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-019-02830-4
更新日期:2020-03-01 00:00:00
abstract::In the application of lasers in dentistry, there is a delicate balance between the benefits gained from laser treatment and the heat-related damage arising from laser irradiation. Hence, it is necessary to understand the different processes associated with the irradiation of lasers on dental materials. To obtain insig...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2251-5
更新日期:2017-08-01 00:00:00
abstract::The aim of this study was to evaluate the degree of conversion (DC) and to identify the viscoelastic properties: storage modulus (E'), loss modulus (E"), tangent delta (tan δ), and glass transition temperature (T g ) of a microhybrid resin-composite light-activated by three different protocols. A Filtek Z250 (3 M ESPE...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-014-1572-x
更新日期:2015-05-01 00:00:00
abstract::This study analyzed the effects of photobiomodulation (PBM) with low-level laser therapy on nociceptive behavior and neuronal activity in the trigeminal nucleus after experimental unilateral temporomandibular joint (TMJ) disc injury. The animals were divided into 4 groups (n = 10 each): group 1, surgical injury of the...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-019-02842-0
更新日期:2020-03-01 00:00:00
abstract:INTRODUCTION:In-transit cutaneous malignant melanoma metastases remains a difficult and distressing disease. It is a sequela to the primary lesion represented by melanoma deposits between the site of excision and the draining lymph nodes. We present our experience of carbon dioxide ablation of in-transit cutaneous mali...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-008-0580-0
更新日期:2009-05-01 00:00:00
abstract::Photosensitizers belonging to the boron-dipyrromethenes (BODIPYs) class were recently found endowed with good efficacy in the antibacterial photodynamic therapy (aPDT) against both Gram-positive and Gram-negative bacteria. In this paper, we report on the remarkable adjuvant effect exerted in this respect by linear pol...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2345-0
更新日期:2018-08-01 00:00:00
abstract::This study aimed to evaluate the effects of low-level laser therapy (LLLT) immediately before tetanic contractions in skeletal muscle fatigue development and possible tissue damage. Male Wistar rats were divided into two control groups and nine active LLLT groups receiving one of three different laser doses (1, 3, and...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-014-1560-1
更新日期:2014-09-01 00:00:00
abstract::We evaluate the safety, efficacy, and oncological outcomes of early palliative photoselective vaporization of the prostate (PVP) by GreenLight high-performance system (HPS) 120-W laser in patients with acute urinary retention (AUR) induced by advanced prostate cancer (PCa). A total of 39 advanced PCa patients with AUR...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-012-1252-7
更新日期:2013-09-01 00:00:00
abstract::To evaluate the effect of various media and Iridex MicroPulse P3 (MP3) probe angles on the power output from the Cyclo G6 Glaucoma Laser (G6) System. A laser power meter was used to measure the power output (milliwatts, mW) of the Cyclo G6 System. Each of the ten trials consisted of measurements in six different media...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03089-w
更新日期:2020-07-18 00:00:00
abstract::The purpose of this in vitro study was to observe the morphological changes and apical dye penetration at apical dentin surfaces after apicoectomy with conventional techniques and laser beam. Sixty single-rooted extracted teeth were selected for the study. The crowns were resected below the cemento-enamel junction. Th...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-007-0497-z
更新日期:2008-10-01 00:00:00
abstract::Wound healing is a very complex process, some phases of which have only recently been explained. Magnetic and electromagnetic fields can modulate this process in a non-thermal way. The aim of this research was to compare the influence of constant and pulsed electromagnetic fields and low-level laser therapy (LLLT) on ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-008-0564-0
更新日期:2009-05-01 00:00:00
abstract::Skin represents an interface between internal and external environment; it protects human body by regulating the water loss and the maintenance of body temperature, defending against irritant and pathogen agents, and against physical, chemical, and UV damage. It provides to essential physiological functions, such as t...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-018-2635-1
更新日期:2019-04-01 00:00:00
abstract::In the present study, we demonstrated the use of nanoporous zinc oxide (ZnO NPs) in photodynamic therapy. The ZnO NPs structure possesses a high surface to volume ratio due to its porosity and ZnO NPs can be used as an efficient photosensitizer carrier system. We were able to grow ZnO NPs on the tip of borosilicate gl...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0989-8
更新日期:2012-05-01 00:00:00
abstract::Various therapies for the treatment of sprains have emerged as advances occur in biomedical engineering and photobiology. Therapy with coherent and non-coherent light is a treatment modality for various musculoskeletal injuries. The main certified phototherapy benefits are the reduction of nociceptive processes and th...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-018-2460-6
更新日期:2018-07-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of infections in humans. Photodynamic therapy using blue light (450 nm) could possibly be used to reduce MRSA on different human tissue surfaces without killing the human cells. It could be less harmful than 300-400 nm light or common disinfectan...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-019-02774-9
更新日期:2019-12-01 00:00:00
abstract::The present work evaluated the effects of LED light irradiation on the healing of the navels of neonatal dairy calves. Fifty-seven neonatal calves were divided into two groups. Animals had their umbilical stumps immersed in 10% iodine tincture for 60 s, and this process was repeated every 24 h for three consecutive da...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2283-x
更新日期:2017-09-01 00:00:00
abstract::High-intensity focused ultrasound (HIFU) can be applied noninvasively to create focused zones of tissue coagulation on various skin layers. We performed a comparative study of HIFU, evaluating patterns of focused tissue coagulation and ablation upon application thereof. A tissue-mimicking (TM) phantom was prepared wit...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-015-1804-8
更新日期:2015-12-01 00:00:00
abstract::Hyperhidrosis and bromhidrosis were lately reported as novel side effects of laser-assisted removal of axillary hair. The goal of our study was to evaluate the reversibility of these two side effects. An observational, single-center cohort study included over a 30-month screening period 30 patients with newly reported...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1404-4
更新日期:2014-03-01 00:00:00
abstract::Low-level red laser (LLRL)-tissue interactions have a wide range of medical applications and are garnering increased attention. Although the positive effects of low-level laser therapy (LLLT) have frequently been reported and enhanced collagen accumulation has been identified as one of the most important mechanisms in...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-015-1823-5
更新日期:2015-12-01 00:00:00
abstract::The purpose of this study is twofold: (1) to determine the feasibility of optical coherence tomography (OCT) to differentiate normal and diseased tissue of the neck region intraoperatively and (2) to evaluate how accurately a cohort of test subjects can identify various tissue types when shown a sample set of OCT imag...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03024-z
更新日期:2020-04-27 00:00:00
abstract::Our objective was to assess the efficacy and safety of combined 10.6 microm and 650 nm laser irradiation on patients with knee osteoarthritis (OA). Forty patients with OA were randomly allocated to an active laser group or to a placebo laser group (20 per group). They either received active or sham laser treatment at ...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-007-0536-9
更新日期:2009-03-01 00:00:00
abstract::Low-level laser therapy (LLLT) has proved to have biostimulating effects on tissues over which they are applied, therefore accelerating the healing process. Most studies in implantology were focused on a reduction of the duration of osseointegration. There exist few articles analyzing the potential effects of these th...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
doi:10.1007/s10103-015-1860-0
更新日期:2016-02-01 00:00:00
abstract::Antimicrobial photodynamic therapy represents an alternative method of killing resistant pathogens. Efforts have been made to develop delivery systems for hydrophobic drugs to improve the photokilling. This study evaluated the photodynamic effect of chloro-aluminum phthalocyanine (ClAlPc) encapsulated in nanoemulsions...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1354-x
更新日期:2015-02-01 00:00:00