Abstract:
:Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of infections in humans. Photodynamic therapy using blue light (450 nm) could possibly be used to reduce MRSA on different human tissue surfaces without killing the human cells. It could be less harmful than 300-400 nm light or common disinfectants. We applied blue light ± riboflavin (RF) to MRSA and keratinocytes, in an in vitro liquid layer model, and compared the effect to elimination using common disinfection fluids. MRSA dilutions (8 × 105/mL) in wells were exposed to blue light (450 nm) ± RF at four separate doses (15, 30, 56, and 84 J/cm2). Treated samples were cultivated on blood agar plates and the colony forming units (CFU) determined. Adherent human cells were cultivated (1 × 104/mL) and treated in the same way. The cell activity was then measured by Cell Titer Blue assay after 24- and 48-h growth. The tested disinfectants were chlorhexidine and hydrogen peroxide. Blue light alone (84 J/cm2) eliminated 70% of MRSA. This dose and riboflavin eradicated 99-100% of MRSA. Keratinocytes were not affected by blue light alone at any dose. A dose of 30 J/cm2 in riboflavin solution inactivated keratinocytes completely. Disinfectants inactivated all cells. Blue light alone at 450 nm can eliminate MRSA without inactivation of human keratinocytes. Hence, a high dose of blue light could perhaps be used to treat bacterial infections without loss of human skin cells. Photodynamic therapy using riboflavin and blue light should be explored further as it may perhaps be possible to exploit in treatment of skin diseases associated with keratinocyte hyperproliferation.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Makdoumi K,Hedin M,Bäckman Adoi
10.1007/s10103-019-02774-9subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1799-1805issue
9eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-019-02774-9journal_volume
34pub_type
杂志文章abstract::There is concern that the delicate fibres and cylindrical diffuser tips used for intraluminal PDT of blood vessels might break during the treatment. We have developed and tested a simple system to monitor for fibre breakage during PDT. Light from the laser is passed through a beam expander, then a beam splitter, and t...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-003-0270-x
更新日期:2003-01-01 00:00:00
abstract::Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-014-1629-x
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journal_title:Lasers in medical science
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abstract::Diabetes mellitus (DM) is associated with musculoskeletal damage. Investigations have indicated that healing of the surgically tenotomized Achilles tendon was considerably augmented following low-level laser therapy (LLLT) in non-diabetic, healthy animals. The aim of the present study was to evaluate the effect of LLL...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-012-1074-7
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abstract::For chronic rhinitis that is refractory to medical therapy, surgical intervention such as endoscopic vidian neurectomy (VN) can be used to control the intractable symptoms. Lasers can contribute to minimizing the invasiveness of ENT surgery. The aim of this retrospective study is to compare in patients who underwent d...
journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1286-5
更新日期:2014-05-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-010-0836-3
更新日期:2011-03-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-016-1921-z
更新日期:2016-07-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-018-2672-9
更新日期:2019-07-01 00:00:00
abstract::Delivery of intradermally focused nanosecond laser pulses with small energy as an alternate technique applicable to clinical procedures in dermatological and plastic surgery is an area of relatively new interest with multiple potential applications. We assessed this approach on common tattoo pigments in dermis in an i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030200025
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journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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更新日期:2019-06-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-007-0526-y
更新日期:2009-01-01 00:00:00
abstract::Outpatient laser ablation of palatine tonsils is a very interesting procedure that has been recently introduced as a routine in head and neck surgery departments. The aim of this study was to describe a new strategy using a Doppler-guided fibre optic neodymium-yttrium-aluminium-garnet (YAG) laser to remove up to 80 % ...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
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更新日期:2013-05-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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journal_title:Lasers in medical science
pub_type: 杂志文章,评审
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journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-006-0389-7
更新日期:2006-09-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/pl00011338
更新日期:2001-01-01 00:00:00
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journal_title:Lasers in medical science
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