Abstract:
:This study was designed to determine the laser dose for the stimulation, zero-bioactivation, and inhibition of normal and neoplastic cells in vitro. The medical use of laser biomodulation has been occurring for decades in the area of tissue healing and inflammatory conditions. The potential to modulate the regeneration and differentiation of early cellular precursors by laser photons is a valuable endeavor searching for novel and efficient methods. A 35-mW HeNe (632.8-nm) laser and power density of 1.25 mW/cm(2) was used to irradiate tissue culture dishes seeded with 400 cells/dish of normal cells (CHO, CCL-226, 3 T3, and HSF) and neoplastic cells (EMT-6 and RIF-1). All cell lines were cultured using DMEM supplemented with 10% and 5% FBS, 2 mM glutamine and 100 U pen-strep antibiotic. Irradiation times of 16, 32, 48, 64, 80, 96, 112, 128, 144, and 160 s for three consecutive days to deliver cumulative doses of 60, 120, 180, 240, 300, 360, 420, 480, 540, and 600 mJ/cm(2) were done, respectively. Cell cultures were stained and colony-forming efficiency was determined. Data analysis was done using Student's t test, α = 0.05. A trend of stimulation, zero-bioactivation, and inhibition in all cell lines was observed except for CCL-226 which gave a pattern of inhibition, zero-bioactivation, and inhibition. The optimum biostimulatory dose was at 180 mJ/cm(2) and bioinhibitory doses were from 420-600 mJ/cm(2) cumulative doses. This study established the dose-dependency of cell growth to laser treatments, that the extent of cellular proliferation is influenced by the type of cells involved, and the risk when laser irradiation is performed on patients with undiagnosed neoplasms and during pregnancy. On the other hand, the ability of laser irradiation to regulate embryonic fibroblasts and human skin fibroblast in vitro suggests possible laser biomodulatory effects on embryonic and adult stem cells directed for tissue regeneration. Studies on the effects of light treatments exploring different laser parameters for the clonal expansion and differentiation of stem cells are recommended.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Al-Watban FA,Andres BLdoi
10.1007/s10103-011-1040-9subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1039-43issue
5eissn
0268-8921issn
1435-604Xjournal_volume
27pub_type
杂志文章abstract::The aim of the study was to investigate the effect of low-level 809 nm laser irradiation on the proliferation rate of human larynx carcinoma cells in vitro. Epithelial tumor cells were obtained from a laryngeal carcinoma and cultured under standard conditions. For laser treatment the cells were spread on 96-well tissu...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-003-0265-7
更新日期:2003-01-01 00:00:00
abstract::The effect of the 488-nm wavelength of argon laser at different power densities and irradiation times on the degree of conversion (DC), temperature rise, water sorption, solubility, flexural strength, flexural modulus, and microhardness of bisphenole A glycol dimethacrylate and triethylen glycol dimethacrylate with a ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-007-0487-1
更新日期:2008-10-01 00:00:00
abstract::The aim of this study was to investigate the effect of different working modes (pulsed and micropulsed) and power settings of a standardized 980-nm diode laser on collateral thermal soft-tissue damage. A total of 108 bovine liver samples were cut with a diode laser at various settings in pulsed and micropulsed mode an...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-1007-x
更新日期:2012-09-01 00:00:00
abstract::The post-stimulation response of neural activities plays an important role to evaluate the effectiveness and safety of neural modulation techniques. Previous studies have established the capability of infrared neural modulation (INM) on neural firing regulation in the central nervous system (CNS); however, the dynamic...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-019-02826-0
更新日期:2020-03-01 00:00:00
abstract::Hyperhidrosis and bromhidrosis were lately reported as novel side effects of laser-assisted removal of axillary hair. The goal of our study was to evaluate the reversibility of these two side effects. An observational, single-center cohort study included over a 30-month screening period 30 patients with newly reported...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-013-1404-4
更新日期:2014-03-01 00:00:00
abstract::A growing number of laboratory and clinical studies over the past 10 years have shown that low-level laser stimulation (633 or 670 nm) at extremely low power densities (about 0.15 mW/cm(2)), when administered through a particular emission mode, is capable of eliciting significant biological effects. Studies on cell cu...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
doi:10.1007/s10103-010-0837-2
更新日期:2011-01-01 00:00:00
abstract::Low-level visible light irradiation was found to stimulate proliferation potential of various types of cells in vitro. Stem cells in general are of significance for implantation in regenerative medicine. The aim of the present study was to investigate the effect of low-level light irradiation on the proliferation of m...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-012-1207-z
更新日期:2013-07-01 00:00:00
abstract::The bactericidal activity of a pulsed fibre-optic Nd-YAP laser on various strains of oral bacteria: Prevotella nigrescens, Streptococcus sanguis, Actinomyces viscosus (i), Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Peptostreptococcus micros (i) was evaluated. A 200-μm optical fibre was placed in...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s101030050010
更新日期:1998-12-01 00:00:00
abstract::Low-intensity laser therapy (LILT) has been considered as a treatment modality in diabetic distal symmetric polyneuropathy (DSP). The aim of this study is to determine the effectiveness of LILT on DSP. We examined 107 subjects with type 2 diabetes for detection of DSP using the Michigan Neuropathy Screening Instrument...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0977-z
更新日期:2011-11-01 00:00:00
abstract::The purpose of this study is to determine the effectiveness of endoscopic diode laser therapy in patients presenting rectal bleeding due to chronic radiation proctitis (CRP). A retrospective analysis of CRP patients who underwent diode laser therapy in a single institution between 2010 and 2016 was carried out. The pa...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2323-6
更新日期:2018-01-01 00:00:00
abstract::The goal of this study was to assess the ablation, coagulation, and carbonization characteristics of the holmium:YAG (Ho:YAG) laser and thulium fiber lasers (TFL). The Ho:YAG laser (100 W av.power), the quasi-continuous (QCW) TFL (120 W av.power), and the SuperPulsed (SP) TFL (50 W av.power) were compared on a non-fro...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03189-7
更新日期:2020-11-11 00:00:00
abstract::In the application of lasers in dentistry, there is a delicate balance between the benefits gained from laser treatment and the heat-related damage arising from laser irradiation. Hence, it is necessary to understand the different processes associated with the irradiation of lasers on dental materials. To obtain insig...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-017-2251-5
更新日期:2017-08-01 00:00:00
abstract::The aim of the present study was to investigate the influence of low-level red (660 nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5 × 104) were incubated in 96-well culture plates. The cells were maintained in M199 medium s...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03016-z
更新日期:2021-02-01 00:00:00
abstract::The objective of this study was to evaluate the microbiological and clinical outcomes following nonsurgical treatment by either scaling and root planing, combination of Nd:YAG and Er:YAG lasers, or by Er:YAG laser treatment alone. The study involved 60 patients with generalized chronic periodontitis, randomly assigned...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10103-018-2491-z
更新日期:2018-09-01 00:00:00
abstract::Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. B...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0945-7
更新日期:2012-01-01 00:00:00
abstract::A disrupted wound repair process often leads to the development of chronic wounds, and pose a major physical, social and economic inconvenience on patients and the public health sector. Chronic wounds are a common complication seen in diabetes mellitus (DM), and often the severity necessitates amputation of the lower ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03041-y
更新日期:2020-06-01 00:00:00
abstract::Bacterial biofilms are often found in chronically infected wounds. Biofilms protect bacteria from antibiotics and impair wound healing. Surgical debridement is often needed to remove the biofilm from an infected wound. Laser-generated shockwave (LGS) treatment is a novel tissue-sparing treatment for biofilm disruption...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03171-3
更新日期:2020-10-27 00:00:00
abstract::The aim of the present study was to evaluate the success rate of failed implants re-implanted after surface treatment with CO2 laser. Despite the widespread use of dental implants, there are many incidents of failures. It is believed that lasers can be applied to decontaminate the implant surface without damaging the ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-016-1921-z
更新日期:2016-07-01 00:00:00
abstract::In this study, the impact of two-dimensional (2D) and three-dimensional (3D) vision on laparoscopic performance of novice surgeons is examined. Twenty-five novice surgeons were directed to complete four basic tasks from European Training in Basic Laparoscopic Urological Skills (E-BLUS) with both 2D and 3D systems in a...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-015-1739-0
更新日期:2015-05-01 00:00:00
abstract::Recurrent herpes labialis is a worldwide life-long oral health problem that remains unsolved. It affects approximately one third of the world population and causes frequent pain and discomfort episodes, as well as social restriction due to its compromise of esthetic features. In addition, the available antiviral drugs...
journal_title:Lasers in medical science
pub_type: 杂志文章,评审
doi:10.1007/s10103-013-1311-8
更新日期:2014-07-01 00:00:00
abstract::Alopecia areata (AA) is a common autoimmune disease that presents with non-scarring alopecia. It is characterized by intra- or peri-follicular lymphocytic infiltrates composed of CD4+ and CD8+ T-cells on histology. To this day, few treatments are effective for AA. Here we present findings of using a low-level laser co...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0953-7
更新日期:2012-03-01 00:00:00
abstract::Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-014-1629-x
更新日期:2015-08-01 00:00:00
abstract::Laser excision has been used with increasing success to treat oral leukoplakia. The aim of the present study was to evaluate whether clinical healing of a leukoplakia after laser surgery is associated with a normal functional status of the new epithelium and whether pathological alterations in these parameters are rel...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
doi:10.1007/s10103-011-0941-y
更新日期:2012-01-01 00:00:00
abstract::We report the results of endoscopic laser therapy on 59 patients with upper gastrointestinal vascular ectasia. After 300 sessions, complications were two nonbleeding and three bleeding ulcers, treated successfully with endoscopic therapy. After treatment, blood transfusions were abolished in 61% and reduced in 22% of ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-006-0389-7
更新日期:2006-09-01 00:00:00
abstract::Endometriosis is a chronic disease affecting mainly women of the reproductive age. Its most common manifestations include impaired fecundity, pelvic pain, and dyschezia. Laparoscopic removal of endometriotic foci remains to be the gold standard for the treatment of endometriosis. More effective techniques of endoscopi...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-014-1630-4
更新日期:2015-01-01 00:00:00
abstract::Primary hyperparathyroidism (HPT) in 80% of patients is due to a solitary parathyroid adenoma, while in 20% multigland pathology exists, usually hyperplasia [Scott-Coombes, Surgery, 21(12):309-312, 2003]. Despite recent advances in minimally invasive parathyroidectomy, better preoperative localisation techniques and i...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-006-0397-7
更新日期:2006-12-01 00:00:00
abstract::The aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660 nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All an...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-018-2627-1
更新日期:2019-04-01 00:00:00
abstract::Topical anaesthetic cream (TAC) is commonly used as a pre-treatment of ablative fractional resurfacing (AFR) laser. Most of anaesthetic cream contains distilled water as major component. Therefore, pre-operative TAC may interfere the photothermal reaction in the skin treated with fractional carbon-dioxide (FCO2) laser...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
doi:10.1007/s10103-016-2112-7
更新日期:2017-02-01 00:00:00
abstract::Zirconia has been regarded as a promising material for dental implants, and Nd:YAG laser treatment has been proposed as a potential strategy to improve its bioactivity. The main aim of the present study was to evaluate the in vitro behavior of human fetal osteoblasts in contact with laser-textured zirconia implant sur...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03066-3
更新日期:2020-12-01 00:00:00
abstract::To identify the best low level laser photobiomodulation application site at the same irradiation time to increase the viability of the skin flap in rats. Eighteen male rats (Rattus norvegicus: var. Albinus, Rodentia Mammalia) were randomly distributed into three groups (n = 6). Group I (GI) was submitted to simulated ...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-018-2694-3
更新日期:2019-07-01 00:00:00