Abstract：
:The aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660 nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to SN crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30 s, 133 J/cm2 fluence). The hind paw was removed and the SN was dissected and positioned on an aluminum support to collect dispersive Raman spectra (830 nm excitation, 30 s accumulation). To estimate the biochemical changes in the SN associated with LLLT, the principal component analysis (PCA) was applied. The Raman spectra of the sciatic nerve fragments showed peaks of the major biochemical components of the nerve, especially sphingolipids, phospholipids, glycoproteins, and collagen. The spectral features identified in some of the principal component loading vectors are referred to the biochemical elements present on the SN and were increased in the groups treated with LLLT, mainly lipids (sphingo and phospholipids) and proteins (collagen)-constituents of the myelin sheath. The RS was effective in identifying the biochemical differences in the SN after the crush injury, and LASER 660 nm was more efficient than the LASER 808 nm in cell proliferation and repair of the injured SN.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
de Almeida Melo Maciel Mangueira M,Maciel Mangueira N,Pereira Gama Filho O,Moysés de Oliveira M,Albuquerque Heluy R,Silveira L Jr,Caparelli Moniz de Aragão Dáquer Edoi
10.1007/s10103-018-2627-1subject
Has Abstractpub_date
2019-04-01 00:00:00pages
525-535issue
3eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-018-2627-1journal_volume
34pub_type
杂志文章abstract：:This study analyzed the effects of photobiomodulation (PBM) with low-level laser therapy on nociceptive behavior and neuronal activity in the trigeminal nucleus after experimental unilateral temporomandibular joint (TMJ) disc injury. The animals were divided into 4 groups (n = 10 each): group 1, surgical injury of the...
journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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pub_type： 杂志文章,评审
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更新日期：2016-08-01 00:00:00
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journal_title：Lasers in medical science
pub_type： 杂志文章
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
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更新日期：2015-01-01 00:00:00
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
pub_type： 杂志文章,随机对照试验
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更新日期：2019-06-01 00:00:00
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journal_title：Lasers in medical science
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journal_title：Lasers in medical science
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更新日期：2020-06-01 00:00:00
abstract：:Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. B...
journal_title：Lasers in medical science
pub_type： 杂志文章
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abstract：:Photodynamic therapy (PDT) is used for the prevention and treatment of non-melanoma skin cancer. Until recently, clinically approved indications have been restricted to actinic keratoses, nodular and superficial basal cell carcinoma, and, since 2006, Bowen disease. However, the range of indications has been expanding ...
journal_title：Lasers in medical science
pub_type： 杂志文章,评审
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