Effects of the combination of low-level laser therapy and anionic polymer membranes on bone repair.

Abstract:

:In view of the limitations of bone reconstruction surgeries using autologous grafts as a gold standard, tissue engineering is emerging as an alternative, which permits the fabrication and improvement of scaffolds to stimulate osteogenesis and angiogenesis, processes that are essential for bone repair. Polymers are used to mimic the extracellular bone matrix and support cell growth. In addition, bone neoformation can be induced by external factors such as laser irradiation, which stimulates bone metabolism. The objective of this study was to evaluate the regeneration of bone defects using collagen and elastin membranes derived from intestinal serosa and bovine auricular cartilage combined with low-level laser application. Thirty-six Wistar rats were operated to create a 3-mm defect in the distal metaphysis of the left femur and divided into six groups: G1 (control, no treatment); G2 (laser); G3 (elastin graft), G4 (elastin+laser); G5 (collagen graft); G6 (collagen+laser). The animals were sacrificed 6 weeks after surgery and the femurs were removed for analysis of bone repair. Macroscopic and radiological results showed the absence of an infectious process in the surgical area. This was confirmed by histological analysis, which revealed no inflammatory infiltrate. Histomorphometry showed that the formation of new bone started from the margins of the bone defect and its volume was greater in elastin+laser and collagen+laser. We conclude that newly formed bone in the graft area was higher in the groups that received the biomaterials and laser. The collagen and elastin matrices showed biocompatibility.

journal_name

Lasers Med Sci

authors

Dos Santos DA,de Guzzi Plepis AM,da Conceição Amaro Martins V,Cardoso GBC,Santos AR Jr,Iatecola A,Andrade TN,Monteiro FM,Calegari ARA,Chacon EL,Cunha MR

doi

10.1007/s10103-019-02864-8

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

813-821

issue

4

eissn

0268-8921

issn

1435-604X

pii

10.1007/s10103-019-02864-8

journal_volume

35

pub_type

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