Abstract:
AIMS:Hypoxia is implicated in the pathogenesis of rheumatoid arthritis (RA), contributing to the tumor-like phenotypes of RA fibroblast-like synoviocytes (RA-FLSs). Andrographolide is the main bioactive component of Andrographis paniculata, an herbal medicine that shows therapeutic benefits in RA patients. Here, we explored the effects of andrographolide on hypoxia-induced migration and invasion of RA-FLSs. MATERIALS AND METHODS:RA-FLSs were exposed to hypoxia in the presence or absence of andrographolide and cell migration and invasion were tested by Transwell assays. The expression of hypoxia-inducible factor-1 alpha (HIF-1α), matrix metalloproteinase (MMP)-1, MMP-3 and MMP-9 was measured by semi-quantitative reverse transcription polymerase chain reaction and Western blot analysis. HIF-1α DNA binding activity was assessed by electrophoretic mobility shift assay. The effects of overexpression of exogenous HIF-1α on the action of andrographolide in RA-FLSs were investigated. KEY FINDINGS:Andrographolide inhibited FLS migration and invasion under hypoxic conditions in a dose-dependent manner. The upregulation of MMP-1, MMP-3 and MMP-9 in response to hypoxia was significantly (P<0.05) attenuated by andrographolide. Moreover, the expression and DNA binding activity of HIF-1α were dose-dependently decreased in andrographolide-treated cells under hypoxic conditions. Overexpression of HIF-1α almost completely reversed the suppressive effects of andrographolide on the migration, invasion and MMP expression of hypoxic RA-FLSs. SIGNIFICANCE:These results indicate the ability of andrographolide to attenuate hypoxia-induced invasiveness of RA-FLSs via inhibition of HIF-1α signaling, and warrant further exploration of andrographolide for the treatment of RA.
journal_name
Life Scijournal_title
Life sciencesauthors
Li GF,Qin YH,Du PQdoi
10.1016/j.lfs.2015.06.019subject
Has Abstractpub_date
2015-09-01 00:00:00pages
67-72eissn
0024-3205issn
1879-0631pii
S0024-3205(15)00343-4journal_volume
136pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::To determine the mechanism of anoxic vasoconstriction, precontracted rat aortic rings were exposed to 95% N2, which caused additional contraction. Reoxygenation (95% O2) resulted in initial relaxation followed by contraction. Indomethacin did not affect anoxic contraction or reoxygenation-mediated events, but NG-monom...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90316-4
更新日期:1991-01-01 00:00:00
abstract:AIMS:Lithium is a common mood stabilizer to treat bipolar disorder. It has a narrow window of therapeutic action and its mechanism of action and possible side effects are still not fully understood. Lithium is a potent inhibitor of glycogen synthase kinase 3β (GSK-3β). Previous studies indicated that lithium can induce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.07.027
更新日期:2012-09-24 00:00:00
abstract::Resting pituitary levels of beta-endorphin-(beta-EP-IR), ACTH-(ACTH-IR), and alpha-MSH-(alpha-MSH-IR)-like immunoreactive material were found to differ among 16 inbred mouse strains. Hormone levels correlated genetically with severity of withdrawal from ethanol, which also differed among the strains. Ethanol dependenc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90672-0
更新日期:1983-11-07 00:00:00
abstract:AIMS:Few studies have compared the interaction of single and repeated administration of amitriptyline (amit) with the nitrergic system and glutamatergic system in the experimental model of neuropathic pain. We aimed to evaluate the antinociceptive effect of single and repeated administration of amit and to assess wheth...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116752
更新日期:2019-09-15 00:00:00
abstract:AIMS:To better understand mechanisms whereby Ajulemic acid (AjA), a synthetic antiinflammatory cannabinoid, promotes resolution of acute and chronic inflammation in animal models, we investigated its influence on cyclooxygenase 2 (COX2) expression and eicosanoid production in human fibroblast-like synovial cells (FLS)....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.09.004
更新日期:2008-11-07 00:00:00
abstract::gamma-Aminobutyric acid (GABA) receptor-mediated 36chloride (36Cl-) uptake was measured in synaptoneurosomes from rat brain. GABA and GABA agonists stimulated 36Cl- uptake in a concentration-dependent manner with the following order of potency: Muscimol greater than GABA greater than piperidine-4-sulfonic acid (P4S)gr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90207-4
更新日期:1987-09-07 00:00:00
abstract::The aim of the present study was to investigate the existence of aging- and sex-related alterations in the permeability of the blood-brain barrier (BBB) in the rat, by calculating a unidirectional blood-to-brain transfer constant (Ki) for the circulating tracer [14C]-alpha-aminoisobutyric acid. We observed that: a) th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90157-m
更新日期:1990-01-01 00:00:00
abstract::We analyzed PGE2 production in primary-cultured human hepatic macrophages (HHM phi) and peripheral monocytes (MO) from patients with and without liver cirrhosis, and correlated PGE2 production with the patients' liver function. Serum choline esterase (ChE) levels were used as an indicator of liver function. PGE2 produ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90751-n
更新日期:1993-01-01 00:00:00
abstract::Three cholecystokinin type B (CCKB) receptor antagonists were labelled with 11C and evaluated ex vivo in rat biodistribution studies. The CCKB antagonists were YF 476 and two other compounds of the basic 3-ureido-1,4-benzodiazepine class. Following tail-vein administration of [11C]-YF 476 exceedingly low levels of rad...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)01021-3
更新日期:2001-02-02 00:00:00
abstract::This study was designed to evaluate the synergistic cytotoxicity of herb prescription, Palgin, in adriamycin-treated cancer cells. The combination of Palgin and adriamycin synergistically augmented the cytotoxicity of Chang and HL-60 cells, but not in Hep3B and Alexander cells. The cytotoxicity of two drugs was reveal...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02039-8
更新日期:2002-10-04 00:00:00
abstract::The muscarinic pharmacology of C1-methyl-substituted chiral compounds related to McN-A-343 and of (R)- and (S)-dimethindene has been studied. Among the McN-A-343 analogues, the (S)-enantiomers were more potent and had higher affinity than the (R)-isomers. The quaternary compound (S)-BN 228 was found to be the most pot...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00015-x
更新日期:1995-01-01 00:00:00
abstract::Previous studies have indicated that one of the causes of alveolar bone destruction with periodontitis is lipopolysaccharide (LPS) from the cell wall of gram-negative bacteria in plaque, and that prostaglandin E(2) (PGE(2)) is one of the bone resorption factors that stimulate osteoclast formation through an intercellu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.09.019
更新日期:2006-03-20 00:00:00
abstract::The effect of gamma-aminobutyric acid (GABA) on the binding of PK 8165, a quinoline derivative, and CGS 8216, a pyrazoloquinoline, was assessed in two different regions of the rat brain. PK 8165, a compound with reported anxiolytic properties, inhibited [3H]-propyl beta-carboline-3-carboxylate labeled receptors in the...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90403-9
更新日期:1982-07-05 00:00:00
abstract::The intestinal mucosa of infant rats was found to produce ketones when incubated in Krebs-Ringer-Bicarbonate solution. No production was found in weaned rats. Ketogenesis could be inhibited by D-carnitine or tetradecylglycidic acid (TDGA) an inhibitor of long-chain acylcarnitine transferase, suggesting that ketone pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90718-1
更新日期:1987-09-21 00:00:00
abstract:AIMS:Hypercholesterolemia is a hazard for increasing susceptibility of the heart to myocardial infarction (MI) by inducing platelet hyperaggregability. Clopidogrel and prasugrel have documented cardioprotective effects in clinical studies. Herein, we investigated whether clopidogrel and prasugrel could protect against ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117429
更新日期:2020-04-15 00:00:00
abstract::The profile of endogenous opioid peptides in the peptide-rich fraction obtained from a homogenate of an ACTH-secreting human pituitary tumor is presented. Gradient RP-HPLC is used to separate the mixture into peptide constituents. A preparation of opioid receptors is used in a radioreceptor assay with ethorphine - a r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90225-5
更新日期:1985-11-11 00:00:00
abstract::Progressive lysis of bone in loose total hip replacement has been ascribed to the capacity of the synovial-like membrane present at the bone interface to produce prostaglandin E2 and cytokines such as tumor necrosis factor (TNF alpha). Phospholipase A2 (PLA2) produces rate limiting precursor i.e. arachidonic acid in t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00342-6
更新日期:1996-01-01 00:00:00
abstract::To investigate the difference of metabolic pharmacokinetics between pure glycyrrhizin (GZ) and GZ in licorice decoction, six New Zealand White rabbits were orally given pure GZ and licorice decoction containing equivalent content of GZ in a randomized crossover design. HPLC methods were used for the quantitation of GZ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.10.020
更新日期:2005-01-21 00:00:00
abstract::Bufalin, which is one of prominent components of Chinese toad venom, was found to decrease the rate of cell proliferation of mouse melanoma clone B16-F10 cells and a concomitant stimulation of expression of its melanotic phenotype. The effect of bufalin on melanogenesis included stimulation of tyrosinase activity and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90213-9
更新日期:1992-01-01 00:00:00
abstract:AIMS:Insulin receptor signaling in osteoblasts has been well established, but the effects of insulin on osteoclast proliferation are poorly explored. The objective of this study was to investigate the roles and the mechanisms of insulin on osteoclast proliferation. MAIN METHODS:After insulin treatment to primary osteo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.07.006
更新日期:2014-10-02 00:00:00
abstract::Responses of atrial mRNA, atrial peptide and plasma peptide of atrial natriuretic factor (ANF) to treatments to alter fluid volume were studied in rats using RNA dot hybridization assay and radioimmunoassay. Specific changes in the level of ANF mRNA relative to total atrial RNA were observed in atria from sodium restr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90157-2
更新日期:1985-05-13 00:00:00
abstract::A monoclonal antibody (C351) against alpha human atrial natriuretic polypeptide (alpha hANP) recognizing human form ring structure was established and applied to a radioimmunoassay of plasma alpha hANP. The minimum detectable amount in terms of 10% radioligand displacement relative to zero dose were 0.28 fmol/tube, co...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90176-2
更新日期:1988-01-01 00:00:00
abstract::Nitric oxide (NO) is a potent regulator in the cardiovascular system; it is generated by the nitric oxide synthase (NOS) family of proteins. NO produced in endothelial cells plays a crucial role in vascular functions. The aim of this study was to clarify the effect of diabetes on aortic NO synthesis in a model of gene...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.010
更新日期:2005-07-15 00:00:00
abstract::Psychostimulants including amphetamine and cocaine induce locomotion and stereotypy and suppress eating. Although the capacity of cocaine to alter locomotion is usually viewed as related to dopamine neurotransmission, recent studies suggest that norepinephrine, acting through alpha1-adrenergic receptors (alpha1-ARs) c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.004
更新日期:2007-09-08 00:00:00
abstract::The anticonvulsant effects and duration of protective action of midazolam against Metrazol induced seizures were studied in 528 rats aged 7,12,18,25 and 90 days. The doses of 0.025, 0.05, 0.25, 0.5 and 1.0 mg/kg were administered immediately before Metrazol (100 mg/kg in all but 18-day-old animals where 90 mg/kg were ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90421-k
更新日期:1992-01-01 00:00:00
abstract::When s-triazolo[3,4-a]phthalazine (Tri-P) was orally administered in rats, a more lipophilic metabolite M-1 than the parent compound was isolated from the urine. The metabolite M-1 was identified as 7-methylthio Tri-P by means of high resolution MS and two-dimensional NMR spectrometry. Furthermore, the 7-methylthio co...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.06.032
更新日期:2003-11-21 00:00:00
abstract::Two-dimensional and line-scan analyses of the early phase Ca2+ transients in rat cardiomyocytes were performed with a rapid-scanning laser confocal microscope and fluo-3 to elucidate the mechanism of activation of Ca2+ release from the sarcoplasmic reticulum in atrial myocytes which lack a well developed T-tubular net...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01436-9
更新日期:2001-12-28 00:00:00
abstract::The immunosuppressive effects of microcolin A, a lipopeptide extracted from the marine blue green alga Lyngbya majuscula were investigated. Microcolin A suppressed concanavalin A (IC50 = 5.8 nM), phytohemagglutinin (IC50 = 12.5 nM) and lipopolysaccharide (IC50 = 8.0 nM) induced proliferation of murine splenocytes. Mix...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00645-5
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Polychlorinated biphenyls (PCBs) are persistent organic pollutants that bioaccumulate in adipose tissue, disturbing its metabolism and the balance of adipokines, related to obesity. The altering secretion pattern of adipokines from the adipose tissue and the increasing mechanical load in weight-bearing joint...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.06.010
更新日期:2016-07-15 00:00:00
abstract::Rats treated chronically with the anticancer agent adriamycin (1.5 mg/kg/week X 14 weeks) exhibited cardiac and renal lesions typical of anthracycline toxicity, and had serum hyperlipidemia characterized by 4 to 10 fold elevations in all lipoprotein classes. Heparin-releasable lipoprotein lipase activity measured in p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90167-5
更新日期:1985-02-18 00:00:00