当前位置： SCI文献检索 > VACCINE期刊下所有文献 > An LASV GPC pseudotyped virus based reporter system enables evaluation of vaccines in mice under non-BSL-4 conditions.

An LASV GPC pseudotyped virus based reporter system enables evaluation of vaccines in mice under non-BSL-4 conditions.

Abstract：

:Lassa virus (LASV) causes a severe hemorrhagic fever endemic throughout western Africa. Because of the ability to cause lethal disease in humans, limited treatment options, and potential as a bioweapon, the need for vaccines to prevent LASV epidemic is urgent. However, LASV vaccine development has been hindered by the lack of appropriate small animal models for efficacy evaluation independent of biosafety level four (BSL-4) facilities. Here we generated an LASV-glycoprotein precursor (GPC)-pseudotyped Human immunodeficiency virus containing firefly luciferase (Fluc) reporter gene as surrogate to develop a bioluminescent-imaging-based BALB/c mouse model for one-round infection under non-BSL-4 conditions, in which the bioluminescent intensity of Fluc was utilized as endpoint when evaluating vaccine efficacy. Electron microscopy analysis demonstrated that LASV GPC pseudotyped virus appeared structurally similar to native virion. Meanwhile, we constructed DNA vaccine (pSV1.0-LASVGPC) and pseudoparticle-based vaccine (LASVpp) that displayed conformational GPC protein of LASV strain Josiah to vaccinate BALB/c mice using intramuscular electroporation and by intraperitoneal routes, respectively. Vaccinated mice in LASVpp alone and DNA prime+LASVpp boost schedules were protected against 100 AID50 of LASV pseudovirus challenge, and it was found that in vivo efficiencies correlated with their anti-LASV neutralizing activities and MCP-1 cytokine levels in serum sampled before infection. The bioluminescence pseudovirus infection model can be useful tool for the preliminary evaluation of immunogenicity and efficacy of vaccine candidates against LASV outside of BSL-4 containments, and the results with pseudoparticle-based vaccine provided very helpful information for LASV vaccine design.

journal_name

Vaccine

journal_title

Vaccine

authors

Li Q,Liu Q,Huang W,Wu J,Nie J,Wang M,Zhao C,Zhang L,Wang Y

doi

10.1016/j.vaccine.2017.07.101

subject

Has Abstract

pub_date

2017-09-12 00:00:00

pages

5172-5178

issue

38

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(17)31037-X

journal_volume

35

pub_type

杂志文章

相关文献

VACCINE文献大全
  • The effect of early measles vaccination on thymic size. A randomized study from Guinea-Bissau.

    abstract：UNLABELLED:In low-income countries early measles vaccine (MV) is associated with reduced child mortality which cannot be explained by prevention of measles. A large thymus gland in infancy is also associated with reduced mortality. We hypothesized that early MV is associated with increased thymic size. Within a randomi...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2014.01.034

    authors： Christensen LD,Eriksen HB,Biering-Sørensen S,Balé C,Do VA,Andersen A,Martins CL,Sodemann M,Aaby P,Benn CS

    更新日期：2014-03-26 00:00:00

  • Seroprevalence of Bordetella pertussis antibodies and anti-pertussis antibody response after a single dose of reduced-antigen combined diphtheria, tetanus, and acellular pertussis vaccine (Tdap) in pregnant Thai women.

    abstract：BACKGROUND:Maternal immunization with tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis vaccine (Tdap) has recently been implemented to prevent infant pertussis. Tdap is still not routinely recommended in Thailand, and there are limited data to support or challenge this strategy. OBJECTIVES:The primar...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.01.074

    authors： Sompagdee N,Anuwutnavin S,Phongsamart W,Senawong S,Umrod P,Robkhonburi A

    更新日期：2020-03-10 00:00:00

  • Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope.

    abstract：:The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV N) is one of the major targets for SARS vaccine due to its high potency in triggering immune responses. In this study, we have identified a novel HLA-A*0201 restricted epitope, N220 (LALLLLDRL), of the SARS-CoV N-protein through bioinform...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.05.025

    authors： Cheung YK,Cheng SC,Sin FW,Chan KT,Xie Y

    更新日期：2007-08-10 00:00:00

  • Human papillomavirus-like particles mediate functional delivery of plasmid DNA to antigen presenting cells in vivo.

    abstract：:Because recombinant empty viral capsids are potentially attractive vectors for gene therapy, here we examined the ability of human papillomavirus (HPV) virus-like particles (VLPs) to mediate delivery and expression of DNA plasmids in vitro and in vivo. VLP-mediated delivery and expression of a GFP reporter construct i...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.01.067

    authors： Malboeuf CM,Simon DA,Lee YE,Lankes HA,Dewhurst S,Frelinger JG,Rose RC

    更新日期：2007-04-30 00:00:00

  • TLR-based immune adjuvants.

    abstract：:This work describes the nature and strength of the immune response induced by various Toll-like receptor ligands and their ability to act as vaccine adjuvants. It reviews the various ligands capable of triggering individual TLRs, and then focuses on the efficacy and safety of those agents for which clinical results ar...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2010.08.002

    authors： Steinhagen F,Kinjo T,Bode C,Klinman DM

    更新日期：2011-04-12 00:00:00

  • Humoral immunity 5 years after booster immunization with an adolescent and adult formulation combined tetanus, diphtheria, and 5-component acellular pertussis vaccine.

    abstract：:Persistence of antibodies following a single dose of Tdap vaccine (tetanus, diphtheria, and five-component acellular pertussis vaccine for use in individuals past childhood) was evaluated in a follow-up of adolescents (N=324) and adults (N=644) who had received Tdap in earlier clinical trials. Outcome measures were se...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.09.031

    authors： Barreto L,Guasparini R,Meekison W,Noya F,Young L,Mills E

    更新日期：2007-11-23 00:00:00

  • Reverse vaccinology, a genome-based approach to vaccine development.

    abstract：:The conventional approach to vaccine development requires cultivation of the pathogenic microorganism and its dissection using biochemical, immunological, and microbiological methods in order to identify the components important for immunity. This method, while successful in many cases, failed to provide a solution fo...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(00)00554-5

    authors： Rappuoli R

    更新日期：2001-03-21 00:00:00

  • Disparities in parental human papillomavirus (HPV) vaccine awareness and uptake among adolescents.

    abstract：:Trends in HPV vaccine awareness among parents of adolescent girls and boys (ages 13-17) and HPV vaccine uptake (≥1 dose) among girls (ages 13-17) were evaluated in Los Angeles County, California. Between 2007 and 2011, parental HPV vaccine awareness increased from 72% to 77% overall, with significant increases among m...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.08.046

    authors： Nonzee NJ,Baldwin SB,Cui Y,Singhal R

    更新日期：2018-02-28 00:00:00

  • Inadequate response to intradermal hepatitis A vaccine.

    abstract：:To assess the efficacy of the intradermal route of administration of hepatitis A vaccine we conducted a study in hospital laboratory workers. Forty-three volunteers were given three different combinations of intradermal and intramuscular hepatitis A vaccine and compared with 18 controls given intramuscular vaccine onl...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0264-410x(94)90303-4

    authors： Brindle RJ,Morris CA,Berger R,Kurtz JB

    更新日期：1994-05-01 00:00:00

  • Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection.

    abstract：:Bordetella parapertussis, a close related species of B. pertussis, can also cause the disease named pertussis or whooping cough. The number of cases caused by this related pathogen has risen sustained in the last years. The widely used cellular (wP) or acellular (aP) pertussis vaccines have little or no efficacy again...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.08.059

    authors： Bottero D,Gaillard ME,Errea A,Moreno G,Zurita E,Pianciola L,Rumbo M,Hozbor D

    更新日期：2013-10-25 00:00:00

  • A phase I dose escalation trial of vaccine replicon particles (VRP) expressing prostate-specific membrane antigen (PSMA) in subjects with prostate cancer.

    abstract：:PSMA-VRP is a propagation defective, viral replicon vector system encoding PSMA under phase I evaluation for patients with castration resistant metastatic prostate cancer (CRPC). The product is derived from an attenuated strain of the alphavirus, Venezuelan Equine Encephalitis (VEE) virus, and incorporates multiple re...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.11.096

    authors： Slovin SF,Kehoe M,Durso R,Fernandez C,Olson W,Gao JP,Israel R,Scher HI,Morris S

    更新日期：2013-01-30 00:00:00

  • Potentiation of an anthrax DNA vaccine with electroporation.

    abstract：:DNA vaccines are a promising method of immunization against biothreats and emerging infections because they are relatively easy to design, manufacture, store and distribute. However, immunization with DNA vaccines using conventional delivery methods often fails to induce consistent, robust immune responses, especially...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.03.064

    authors： Luxembourg A,Hannaman D,Nolan E,Ellefsen B,Nakamura G,Chau L,Tellez O,Little S,Bernard R

    更新日期：2008-09-19 00:00:00

  • HPV vaccine for adolescent males: acceptability to parents post-vaccine licensure.

    abstract：:We examined mothers' willingness to get their adolescent sons HPV vaccine. In December 2009, 2 months after approval of HPV vaccine for males, we surveyed a national sample of mothers with sons aged 9-18 (n=406). More mothers were definitely or probably willing to get their sons HPV vaccine if the vaccine were free (4...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.06.114

    authors： Reiter PL,McRee AL,Gottlieb SL,Brewer NT

    更新日期：2010-08-31 00:00:00

  • Elucidation of the full genetic information of Japanese rubella vaccines and the genetic changes associated with in vitro and in vivo vaccine virus phenotypes.

    abstract：:Rubella is a mild disease characterized by low-grade fever, and a morbilliform rash, but causes congenital defects in neonates born from mothers who suffered from rubella during the pregnancy. After many passages of wild-type rubella virus strains in various types of cultured cells, five live attenuated rubella vaccin...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.01.016

    authors： Otsuki N,Abo H,Kubota T,Mori Y,Umino Y,Okamoto K,Takeda M,Komase K

    更新日期：2011-02-24 00:00:00

  • A therapeutic HIV-1 vaccine reduces markers of systemic immune activation and latent infection in patients under highly active antiretroviral therapy.

    abstract：:HIV infection is characterized by chronic immune activation and the establishment of a pool of latently infected cells. Antiretroviral therapy (ART) can suppress viral load to undetectable levels in peripheral blood by standard measure, however immune activation/chronic inflammation and latent infection persist and af...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.04.015

    authors： Pallikkuth S,Bolivar H,Fletcher MA,Babic DZ,De Armas LR,Gupta S,Termini JM,Arheart KL,Stevenson M,Tung FY,Fischl MA,Pahwa S,Stone GW

    更新日期：2020-06-02 00:00:00

  • Invasive pneumococcal diseases in birth cohorts vaccinated with PCV-7 and/or PHiD-CV in the province of Quebec, Canada.

    abstract：BACKGROUND:The 10-valent protein D pneumococcal conjugate vaccine (PHiD-CV) was licensed on the basis of immunogenicity studies and there are no published data on its effectiveness to prevent invasive pneumococcal disease (IPD). In the province of Quebec, Canada, PHiD-CV was introduced in the summer of 2009, replacing ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.08.017

    authors： De Wals P,Lefebvre B,Defay F,Deceuninck G,Boulianne N

    更新日期：2012-10-05 00:00:00

  • Developing a seasonal influenza vaccine recommendation in Kenya: Process and challenges faced by the National Immunization Technical Advisory Group (NITAG).

    abstract：BACKGROUND:In 2014 the Kenya National Immunization Technical Advisory Group (KENITAG) was asked by the Ministry of Health to provide an evidence-based recommendation on whether the seasonal influenza vaccine should be introduced into the national immunization program (NIP). METHODS:We reviewed KENITAG manuals, reports...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.11.062

    authors： Dawa J,Chaves SS,Ba Nguz A,Kalani R,Anyango E,Mutie D,Muthoka P,Tabu C,Maritim M,Amukoye E,Were F,Kenya National Immunization Technical Advisory Group (KENITAG).

    更新日期：2019-01-14 00:00:00

  • Impact of introduction of the 9-valent human papillomavirus vaccine on vaccination coverage of youth in North Carolina.

    abstract：OBJECTIVES:The objective of this study was to evaluate the impact of introduction of 9vHPV vaccine on HPV vaccination uptake (doses per capita) and initiation (≥1 doses), completion (≥3 doses) and compliance (≥3 doses within 12 months) by adolescents. METHODS:We used a retrospective cohort analysis using North Carolin...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.01.013

    authors： Trogdon JG,Shafer P,Lindsay B,Coyne-Beasley T

    更新日期：2018-02-28 00:00:00

  • Amount of maternal rotavirus-specific antibodies influence the outcome of rotavirus vaccination of newborn mice with virus-like particles.

    abstract：:In presence of low or high levels of rotavirus-specific maternal antibodies, the ability of newborn mice to respond to immunization with rotavirus RF 8*-2/6/7 VLPs, was evaluated. After parenteral vaccination, 100% of offspring born to low-antibody-titer dams developed rotavirus-specific IgG antibodies (n=7). In contr...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.11.089

    authors： Johansson E,Istrate C,Charpilienne A,Cohen J,Hinkula J,Poncet D,Svensson L,Johansen K

    更新日期：2008-02-06 00:00:00

  • Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver.

    abstract：:Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immun...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(99)00438-7

    authors： Rogers JV,Hull BE,Fink PS,Chiou HC,Bigley NJ

    更新日期：2000-02-14 00:00:00

  • Strategic health communication on social media: Insights from a Danish social media campaign to address HPV vaccination hesitancy.

    abstract：OBJECTIVE:To evaluate whether the social media strategy developed for the campaign Stop HPV - stop cervical cancer was successful at engaging target groups in communication regarding HPV vaccination. INTRODUCTION:In 2009, the Human Papillomavirus (HPV) vaccine became part of the Danish childhood vaccination program to...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.05.061

    authors： Pedersen EA,Loft LH,Jacobsen SU,Søborg B,Bigaard J

    更新日期：2020-06-26 00:00:00

  • Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine.

    abstract：:The current human whole-cell vaccine is ineffective against pneumonic plague caused by typical F1 capsule positive (F1+) strains of Yersinia pestis. The authors found this vaccine to also be ineffective against F1-negative (F1-) Y. pestis strains, which have been isolated from a human case and from rodents. For these ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)80110-2

    authors： Heath DG,Anderson GW Jr,Mauro JM,Welkos SL,Andrews GP,Adamovicz J,Friedlander AM

    更新日期：1998-07-01 00:00:00

  • Safety and immunogenicity of an improved oral inactivated multivalent enterotoxigenic Escherichia coli (ETEC) vaccine administered alone and together with dmLT adjuvant in a double-blind, randomized, placebo-controlled Phase I study.

    abstract：BACKGROUND:We have developed a new oral vaccine against enterotoxigenic Escherichia coli (ETEC), which is the most common cause of bacterial diarrhea in children in developing countries and in travelers. METHODS:The vaccine was tested for safety and immunogenicity alone and together with double-mutant heat-labile toxi...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2014.10.069

    authors： Lundgren A,Bourgeois L,Carlin N,Clements J,Gustafsson B,Hartford M,Holmgren J,Petzold M,Walker R,Svennerholm AM

    更新日期：2014-12-12 00:00:00

  • Meningococcal-group B (MenB) vaccine series completion and adherence to dosing schedule in the United States: A retrospective analysis by vaccine and payer type.

    abstract：BACKGROUND:Two MenB vaccines with different dosing schedules are approved in the US: MenB-4C (2 doses) and MenB-FHbp (2-3 doses). Both vaccines were licensed on the basis of immunogenicity demonstrated after vaccine series completion. We evaluated vaccination completion and adherence to dosing schedules. METHODS:This ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.06.065

    authors： Packnett E,Irwin DE,Novy P,Watson PS,Whelan J,Moore-Schiltz L,Lucci M,Hogea C

    更新日期：2019-09-16 00:00:00

  • Feasibility study of a combined diphtheria-tetanus-acellular pertussis-hepatitis B (DTPa-HBV) vaccine, and comparison of clinical reactions and immune responses with diphtheria-tetanus-acellular pertussis (DTPa) and hepatitis B vaccines applied as mixed o

    abstract：:The feasibility of a combined diphtheria-tetanus-acellular pertussis-hepatitis B (DTPa-HBV) vaccine was assessed and a comparison made of immunogenicity and reactogenicity to DTPa and HBV vaccines mixed in one syringe and to concomitant but separate injections as a primary vaccination course in three groups of infants...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0264-410x(97)00089-3

    authors： Usonis V,Bakasenas V,Willems P,Clemens R

    更新日期：1997-10-01 00:00:00

  • Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge.

    abstract：:DNA vaccines might offer an alternative to the live smallpox vaccine in providing protective efficacy in an orthopoxvirus (OPV) lethal respiratory challenge model. BALB/c mice were immunised with DNA vaccines coding for 10 different single vaccinia virus (VACV) membrane proteins. After an intranasal challenge with the...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.02.034

    authors： Pulford DJ,Gates A,Bridge SH,Robinson JH,Ulaeto D

    更新日期：2004-09-03 00:00:00

  • Identification of Setaria cervi heat shock protein 70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.

    abstract：:Using mass spectrometry and immunological approaches, a heat shock protein 70 associated with lymphatic filariasis (LF) has been identified from a bovine filarial parasite Setaria cervi. A heat shock protein was detected in different life stages of S. cervi when exposed to an elevated temperature of 44 degrees C. A co...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.06.044

    authors： Srivastava S,Srikanth E,Liebau E,Rathaur S

    更新日期：2010-02-03 00:00:00

  • Novel vaccine concept based on back-boost effect in viral infection.

    abstract：:A novel vaccine concept is discussed based on recent evidence of a "back-boost" effect in Influenza infection. The initial immune response to the infection is imprinted through an immune memory pathway. The immune memory in the back-boost mechanism could be used in reversed order as a "forward-boost" in the proposed v...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.05.035

    authors： Kohler H

    更新日期：2015-06-26 00:00:00

  • Burden and trends of type-specific human papillomavirus infections and related diseases in the Asia Pacific region.

    abstract：:We present the burden of human papillomavirus (HPV)-related cancers (cancers of the cervix, anogenital areas, and oral cavity and pharynx) in terms of incidence and mortality, for the countries of the Asia Pacific region. The region contains more than half of the world population, and manifests a wide geographic diver...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.05.010

    authors： Parkin DM,Louie KS,Clifford G

    更新日期：2008-08-19 00:00:00

  • Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection.

    abstract：:We developed a rapid and sensitive diagnosis system for H5N1 highly pathogenic avian influenza (HPAI) virus infection using an unique gene amplification method, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The sensitivity of the system was found to be 100-fold higher than that of ordinary on...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.05.046

    authors： Imai M,Ninomiya A,Minekawa H,Notomi T,Ishizaki T,Tashiro M,Odagiri T

    更新日期：2006-11-10 00:00:00