The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus-Just a False Positive?

Abstract:

:We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.

journal_name

Mar Drugs

journal_title

Marine drugs

authors

Strand M,Hedström M,Seth H,McEvoy EG,Jacobsson E,Göransson U,Andersson HS,Sundberg P

doi

10.3390/md14040063

subject

Has Abstract

pub_date

2016-03-25 00:00:00

issue

4

issn

1660-3397

pii

md14040063

journal_volume

14

pub_type

杂志文章
  • Molecular Targets of Active Anticancer Compounds Derived from Marine Sources.

    abstract::Over the past decades, a number of novel compounds, which are produced in the marine environment, have been found to exhibit the anticancer effects. This review focuses on molecular targets of marine-derived anticancer candidates in clinical and preclinical studies. They are kinases, transcription factors, histone dea...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md16050175

    authors: Song X,Xiong Y,Qi X,Tang W,Dai J,Gu Q,Li J

    更新日期:2018-05-22 00:00:00

  • Influence of Chitosan Swelling Behaviour on Controlled Release of Tenofovir from Mucoadhesive Vaginal Systems for Prevention of Sexual Transmission of HIV.

    abstract::The main challenges facing efforts to prevent the transmission of human immunodeficiency virus (HIV) are the lack of access to sexual education services and sexual violence against young women and girls. Vaginal formulations for the prevention of sexually transmitted infections are currently gaining importance in drug...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15020050

    authors: Notario-Pérez F,Martín-Illana A,Cazorla-Luna R,Ruiz-Caro R,Bedoya LM,Tamayo A,Rubio J,Veiga MD

    更新日期:2017-02-21 00:00:00

  • A Place to Call Home: An Analysis of the Bacterial Communities in Two Tethya rubra Samaai and Gibbons 2005 Populations in Algoa Bay, South Africa.

    abstract::Sponges are important sources of bioactive secondary metabolites. These compounds are frequently synthesized by bacterial symbionts, which may be recruited from the surrounding seawater or transferred to the sponge progeny by the parent. In this study, we investigated the bacterial communities associated with the spon...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15040095

    authors: Waterworth SC,Jiwaji M,Kalinski JJ,Parker-Nance S,Dorrington RA

    更新日期:2017-03-25 00:00:00

  • The Protective Effects of Clams on Hypercholesterolemia in Late-Stage Triple-Transgenic Alzheimer's Diseased Mice Hearts.

    abstract::To investigate a high cholesterol diet in Alzheimer's disease (AD) mice, they were fed with (2% cholesterol) in five groups with a control group, AD mice group, AD mice plus Meretrix lusoria group, AD mice plus Geloina eros group, and, AD mice plus Corbicula fluminea group for three months, and treated with the fatty ...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md16080263

    authors: Hsieh YL,Teng HJ,Yeh YH,Hsieh CH,Huang CY

    更新日期:2018-08-01 00:00:00

  • Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: the sea urchin Paracentrotus lividus.

    abstract::Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is requir...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md12094912

    authors: Benedetto CD,Barbaglio A,Martinello T,Alongi V,Fassini D,Cullorà E,Patruno M,Bonasoro F,Barbosa MA,Carnevali MD,Sugni M

    更新日期:2014-09-24 00:00:00

  • Effects of long chain fatty acid synthesis and associated gene expression in microalga Tetraselmis sp.

    abstract::With the depletion of global fish stocks, caused by high demand and effective fishing techniques, alternative sources for long chain omega-3 fatty acids are required for human nutrition and aquaculture feeds. Recent research has focused on land-based cultivation of microalgae, the primary producers of omega-3 fatty ac...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md12063381

    authors: Adarme-Vega TC,Thomas-Hall SR,Lim DK,Schenk PM

    更新日期:2014-06-04 00:00:00

  • Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells.

    abstract::N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare alpha-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-alpha production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited t...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md7040589

    authors: Oda T,Lee JS,Sato Y,Kabe Y,Sakamoto S,Handa H,Mangindaan RE,Namikoshi M

    更新日期:2009-11-17 00:00:00

  • Ascidian Toxins with Potential for Drug Development.

    abstract::Ascidians (tunicates) are invertebrate chordates, and prolific producers of a wide variety of biologically active secondary metabolites from cyclic peptides to aromatic alkaloids. Several of these compounds have properties which make them candidates for potential new drugs to treat diseases such as cancer. Many of the...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md16050162

    authors: Watters DJ

    更新日期:2018-05-13 00:00:00

  • Bromoperoxidase Producing Bacillus spp. Isolated from the Hypobranchial Glands of a Muricid Mollusc Are Capable of Tyrian Purple Precursor Biogenesis.

    abstract::The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor ...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md17050264

    authors: Ngangbam AK,Mouatt P,Smith J,Waters DLE,Benkendorff K

    更新日期:2019-05-03 00:00:00

  • Chitosan based polyelectrolyte complexes as potential carrier materials in drug delivery systems.

    abstract::Chitosan has been the subject of interest for its use as a polymeric drug carrier material in dosage form design due to its appealing properties such as biocompatibility, biodegradability, low toxicity and relatively low production cost from abundant natural sources. However, one drawback of using this natural polysac...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md8041305

    authors: Hamman JH

    更新日期:2010-04-19 00:00:00

  • Fucoxanthin-Containing Cream Prevents Epidermal Hyperplasia and UVB-Induced Skin Erythema in Mice.

    abstract::Microalgae represent a source of bio-active compounds such as carotenoids with potent anti-inflammatory and antioxidant properties. We aimed to investigate the effects of fucoxanthin (FX) in both in vitro and in vivo skin models. Firstly, its anti-inflammatory activity was evaluated in LPS-stimulated THP-1 macrophages...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md16100378

    authors: Rodríguez-Luna A,Ávila-Román J,González-Rodríguez ML,Cózar MJ,Rabasco AM,Motilva V,Talero E

    更新日期:2018-10-10 00:00:00

  • Astaxanthin Ameliorates Lipopolysaccharide-Induced Neuroinflammation, Oxidative Stress and Memory Dysfunction through Inactivation of the Signal Transducer and Activator of Transcription 3 Pathway.

    abstract::Astaxanthin (AXT), a xanthophyll carotenoid compound, has potent antioxidant, anti-inflammatory and neuroprotective properties. Neuroinflammation and oxidative stress are significant in the pathogenesis and development of Alzheimer's disease (AD). Here, we studied whether AXT could alleviate neuroinflammation, oxidati...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md17020123

    authors: Han JH,Lee YS,Im JH,Ham YW,Lee HP,Han SB,Hong JT

    更新日期:2019-02-18 00:00:00

  • A Transcriptomic Survey of Ion Channel-Based Conotoxins in the Chinese Tubular Cone Snail (Conus betulinus).

    abstract::Conotoxins in the venom of cone snails (Conus spp.) are a mixture of active peptides that work as blockers, agonists, antagonists, or inactivators of various ion channels. Recently we reported a high-throughput method to identify 215 conotoxin transcripts from the Chinese tubular cone snail, C. betulinus. Here, based ...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15070228

    authors: Huang Y,Peng C,Yi Y,Gao B,Shi Q

    更新日期:2017-07-18 00:00:00

  • Total synthesis of fellutamide B and deoxy-fellutamides B, C, and D.

    abstract::The total syntheses of the marine-derived lipopeptide natural product fellutamide B and deoxy-fellutamides B, C, and D are reported. These compounds were accessed through a novel solid-phase synthetic strategy using Weinreb amide-derived resin. As part of the synthesis, a new enantioselective route to (3R)-hydroxy lau...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md11072382

    authors: Giltrap AM,Cergol KM,Pang A,Britton WJ,Payne RJ

    更新日期:2013-07-08 00:00:00

  • Bioactivity of Spongian Diterpenoid Scaffolds from the Antarctic Sponge Dendrilla antarctica.

    abstract::The Antarctic sponge Dendrilla antarctica is rich in defensive terpenoids with promising antimicrobial potential. Investigation of this demosponge has resulted in the generation of a small chemical library containing diterpenoid secondary metabolites with bioactivity in an infectious disease screening campaign focused...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md18060327

    authors: Bory A,Shilling AJ,Allen J,Azhari A,Roth A,Shaw LN,Kyle DE,Adams JH,Amsler CD,McClintock JB,Baker BJ

    更新日期:2020-06-23 00:00:00

  • Preliminary structure-activity relationship on theonellasterol, a new chemotype of FXR antagonist, from the marine sponge Theonella swinhoei.

    abstract::Using theonellasterol as a novel FXR antagonist hit, we prepared a series of semi-synthetic derivatives in order to gain insight into the structural requirements for exhibiting antagonistic activity. These derivatives are characterized by modification at the exocyclic carbon-carbon double bond at C-4 and at the hydrox...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md10112448

    authors: Sepe V,Ummarino R,D'Auria MV,Taglialatela-Scafati O,Marino SD,D'Amore C,Renga B,Chini MG,Bifulco G,Nakao Y,Fusetani N,Fiorucci S,Zampella A

    更新日期:2012-11-05 00:00:00

  • Sargassum horneri as a Functional Food Ameliorated IgE/BSA-Induced Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mice.

    abstract::Sargassum horneri (S. horneri), an edible brown alga, has been proposed as a functional food with an improvement effect on abnormal skin immune responses. The present study investigates the anti-allergic effect of an ethanol extract from S. horneri (SHE) on immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated ac...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md18120594

    authors: Han EJ,Kim HS,Sanjeewa KKA,Jung K,Jee Y,Jeon YJ,Fernando IPS,Ahn G

    更新日期:2020-11-26 00:00:00

  • Potential anti-HPV and related cancer agents from marine resources: an overview.

    abstract::Recently, the studies on the prevention and treatment of human papillomavirus (HPV) which is closely related to the cervical cancer and other genital diseases are attracting more and more attention all over the world. Marine-derived polysaccharides and other bioactive compounds have been shown to possess a variety of ...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md12042019

    authors: Wang SX,Zhang XS,Guan HS,Wang W

    更新日期:2014-04-03 00:00:00

  • Brevianamides and Mycophenolic Acid Derivatives from the Deep-Sea-Derived Fungus Penicillium brevicompactum DFFSCS025.

    abstract::Four new compounds (1-4), including two brevianamides and two mycochromenic acid derivatives along with six known compounds were isolated from the deep-sea-derived fungus Penicillium brevicompactum DFFSCS025. Their structures were elucidated by spectroscopic analysis. Moreover, the absolute configurations of 1 and 2 w...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15020043

    authors: Xu X,Zhang X,Nong X,Wang J,Qi S

    更新日期:2017-02-17 00:00:00

  • Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice.

    abstract::Application of cytostatics in cancer patients' chemotherapy results in a number of side effects, including the inhibition of various parts of hematopoiesis. Two sulfated polysaccharides, fucoidan from the seaweed Chordaria flagelliformis (PS-Fuc) and fucosylated chondroitin sulfate from the sea cucumber Massinium magn...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15100301

    authors: Anisimova N,Ustyuzhanina N,Bilan M,Donenko F,Usov A,Kiselevskiy M,Nifantiev N

    更新日期:2017-09-30 00:00:00

  • Laminariales Host Does Impact Lipid Temperature Trajectories of the Fungal Endophyte Paradendryphiella salina (Sutherland.).

    abstract::Kelps are colonized by a wide range of microbial symbionts. Among them, endophytic fungi remain poorly studied, but recent studies evidenced yet their high diversity and their central role in algal defense against various pathogens. Thus, studying the metabolic expressions of kelp endophytes under different conditions...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md18080379

    authors: Vallet M,Meziane T,Thiney N,Prado S,Hubas C

    更新日期:2020-07-22 00:00:00

  • Astaxanthin Attenuates Fish Oil-Related Hepatotoxicity and Oxidative Insult in Juvenile Pacific White Shrimp (Litopenaeus vannamei).

    abstract::The present study investigated the effect of dietary astaxanthin (AX) on the growth performance, antioxidant parameters, and repair of hepatopancreas damage in Pacific white shrimp (Litopenaeus vannamei). To evaluate the hepatopancreas protective function of AX in shrimps, we compared the effect of five isonitrogenous...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md18040218

    authors: Yu Y,Liu Y,Yin P,Zhou W,Tian L,Liu Y,Xu D,Niu J

    更新日期:2020-04-17 00:00:00

  • Anti-Coagulant and Anti-Thrombotic Properties of Blacklip Abalone (Haliotis rubra): In Vitro and Animal Studies.

    abstract::Sulphated polysaccharides with anti-thrombotic and anti-coagulant activities have been found in various marine biota. In this study, a previously characterised anti-thrombotic and anti-coagulant extract from blacklip abalone was fractionated by anion exchange chromatography (AEC), pooled (on a sulphated polysaccharide...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15080240

    authors: Suleria HAR,Masci PP,Zhao KN,Addepalli R,Chen W,Osborne SA,Gobe GC

    更新日期:2017-08-04 00:00:00

  • Bromopyrrole alkaloids as lead compounds against protozoan parasites.

    abstract::In the present study, 13 bromopyrrole alkaloids, including the oroidin analogs hymenidin (2), dispacamide B (3) and dispacamide D (4), stevensine (5) and spongiacidin B (6), their derivatives lacking the imidazole ring bromoaldisin (7), longamide B (8) and longamide A (9), the dimeric oroidin derivatives sceptrin (10)...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md8072162

    authors: Scala F,Fattorusso E,Menna M,Taglialatela-Scafati O,Tierney M,Kaiser M,Tasdemir D

    更新日期:2010-07-14 00:00:00

  • Innovative Approach to Sustainable Marine Invertebrate Chemistry and a Scale-Up Technology for Open Marine Ecosystems.

    abstract::Isolation of marine compounds from living invertebrates represents a major challenge for sustainable and environmentally friendly exploitation of marine bio-resources. To develop innovative technology to trap invertebrate compounds in the open sea, the proof of concept of a system combining external continuous circula...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md16050152

    authors: Vlachou P,Le Goff G,Alonso C,Álvarez PA,Gallard JF,Fokialakis N,Ouazzani J

    更新日期:2018-05-06 00:00:00

  • Production of bioactive secondary metabolites by marine vibrionaceae.

    abstract::Bacteria belonging to the Vibrionaceae family are widespread in the marine environment. Today, 128 species of vibrios are known. Several of them are infamous for their pathogenicity or symbiotic relationships. Despite their ability to interact with eukaryotes, the vibrios are greatly underexplored for their ability to...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md9091440

    authors: Mansson M,Gram L,Larsen TO

    更新日期:2011-01-01 00:00:00

  • Bioactive peptides and depsipeptides with anticancer potential: sources from marine animals.

    abstract::Biologically active compounds with different modes of action, such as, antiproliferative, antioxidant, antimicrotubule, have been isolated from marine sources, specifically algae and cyanobacteria. Recently research has been focused on peptides from marine animal sources, since they have been found as secondary metabo...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审

    doi:10.3390/md10050963

    authors: Suarez-Jimenez GM,Burgos-Hernandez A,Ezquerra-Brauer JM

    更新日期:2012-05-01 00:00:00

  • Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains.

    abstract::The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating un...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md13063791

    authors: Merino S,de Mendoza E,Canals R,Tomás JM

    更新日期:2015-06-15 00:00:00

  • Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells.

    abstract::Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given ...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md17030138

    authors: Tseng CC,Lai YC,Kuo TJ,Su JH,Sung PJ,Feng CW,Lin YY,Chen PC,Tai MH,Cheng SY,Kuo HM,Wen ZH

    更新日期:2019-02-27 00:00:00

  • Laucysteinamide A, a Hybrid PKS/NRPS Metabolite from a Saipan Cyanobacterium, cf. Caldora penicillata.

    abstract::A bioactivity guided study of a cf. Caldora penicillata species, collected during a 2013 expedition to the Pacific island of Saipan, Northern Mariana Islands (a commonwealth of the USA), led to the isolation of a new thiazoline-containing alkaloid, laucysteinamide A (1). Laucysteinamide A is a new monomeric analogue o...

    journal_title:Marine drugs

    pub_type: 杂志文章

    doi:10.3390/md15040121

    authors: Zhang C,Naman CB,Engene N,Gerwick WH

    更新日期:2017-04-14 00:00:00