Abstract:
:It has been well established that bone morphogenetic protein-2 (BMP-2) can induce bone formation bothin vivo andin vitro, although high concentrations (up to milligrams) of BMP-2 have been required to achieve this effectin vivo. Further, clinical applications are usually limited to a single dose at the time of implantation. In an attempt to prolong the transforming effect of BMP-2 we used a recombinant adenoviral vector carrying the human BMP-2 gene (Adv-BMP2) to transduce marrow-derived mesenchymal stem cells (MSC) of skeletally mature male New Zealand white rabbits. The pluripotential MSC were incubated with Adv-BMP2 overnight followed by culture in growth medium for 1 week. Assays on tissue cultures demonstrated that these Adv-BMP2 transduced MSC produced BMP-2 protein, differentiated into an osteoprogenitor line, and induced bone formationin vitro. These MSC had increased alkaline phosphatase activity, increased expression of type I collagen, osteopontin, and osteocalcin mRNA, and induced matrix mineralization compared with both nontransduced cells and cells transduced with a control adenoviral construct. To analyze the osteogenic potentialin vivo, Adv-BMP2-transduced MSC were autologously implanted into the intertransverse process space between L5 and L6 of the donor rabbits. The production of new bone was demonstrated by radiographic examination 4 weeks later in areas implanted with cells transduced with Adv-BMP2, whereas no bone was evident at sites implanted with cells transduced with the control adenoviral construct. Histological examination further confirmed the presence of new bone formation. These accumulated data indicate that it is possible to successfully transduce mesenchymal stem cells with a recombinant adenoviral vector carrying the gene for BMP-2 such that these cells will produce BMP-2, differentiate into an osteoprogenitor line, and induce bone formation bothin vitro andin vivo. Moreover, incubation of the Adv-BMP2-transduced cells for an additional 7 days in culture before transplantation enhances the success rate in bone formation (three out of three) as compared with our previous report (one out of five, Calcif Tissue Int 63:357-360, 1998).
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Cheng SL,Lou J,Wright NM,Lai CF,Avioli LV,Riew KDdoi
10.1007/BF02678146subject
Has Abstractpub_date
2001-02-01 00:00:00pages
87-94issue
2eissn
0171-967Xissn
1432-0827pii
10.1007/BF02678146journal_volume
68pub_type
杂志文章abstract::A positional cloning effort in French Canadian families with Paget's disease of bone (PDB) resulted in the identification of a mutation in the sequestosome1 (SQSTM1) gene in a subset of both familial and sporadic PDB cases. This was confirmed in samples of mainly United Kingdom (UK) origin. In this study, we performed...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0244-4
更新日期:2004-08-01 00:00:00
abstract::Twenty boys were followed during their puberty for about 2 years with examinations every third month. At each examination we determined serum concentrations of 25OHD3, 1,25(OH)2D3, 24,25(OH)2D3, 25.26(OH)2D3, alkaline phosphatase (AP) and testosterone together with bone mineral content (BMC) at the distal forearm. Hig...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02556871
更新日期:1986-03-01 00:00:00
abstract::Methotrexate (MTX) is one of the most commonly prescribed drugs for autoimmune rheumatic diseases. As there is no consensus on its negative effects on bone, the purpose of this investigation was to determine the clinical spectrum of patients with stress fractures due to long-term MTX treatment (i.e., MTX osteopathy). ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00765-5
更新日期:2021-02-01 00:00:00
abstract::Radiation therapy (RT) is an established treatment modality for malignant neoplasms. RT induces tissue damage that may lead to osteoradionecrosis in more severe cases. Suitable animal models to study RT-induced changes in membranous craniofacial bone are currently not available. The aim of this study was therefore to ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-009-9217-y
更新日期:2009-05-01 00:00:00
abstract::In adult human bone, fluoride uptake is accompanied by an increase in apatite crystal size. This increase, however, is not isotropic but is restricted primarily to growth in width and/or thickness, with no measurable change in length. In the present study, seeded growth experiments were conducted in vitro to determine...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900522
更新日期:1998-09-01 00:00:00
abstract::We compared indices of three-dimensional microstructure of iliac trabecular bone between 26 patients with vertebral compression fractures due to postmenopausal osteoporosis and 24 control subjects without vertebral fracture, who were matched for age, sex, race, menopausal status, and several densitometric and histolog...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02554913
更新日期:1985-12-01 00:00:00
abstract::To study the mechanism of action of thyroid hormones on bone, we examined the effects of immunosuppressive and nonimmunosuppressive cyclosporins, as well as of transforming growth factor beta 1 (TGF beta 1), 17 beta-estradiol (E2), and dihydroxytestosterone (DHT) on thyroxine (T4)- and triiodothyronine (T3)-stimulated...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298788
更新日期:1992-02-01 00:00:00
abstract::We conducted a case-control study on fracture risk associated with the use of orally administered prednisolone/prednisone, budesonide, methylprednisolone, and hydrocortisone to assess if the various preparations were associated with different fracture patterns. Cases were all subjects with any fracture sustained durin...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-008-9124-7
更新日期:2008-04-01 00:00:00
abstract::Dual-photon absorptiometry (153Gd) was used to measure bone mineral of the total body and major anatomical areas. Patients with osteoporosis (male = 11, female = 18) and with renal osteodystrophy (n = 17) were significantly below (20%) normal females (n = 72) and males (n = 13) at most sites. In the osteoporotic patie...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02405287
更新日期:1984-01-01 00:00:00
abstract::Using both low microwave power and weak magnetic field modulation, we have shown that the asymmetric signal arising in X-irradiated tooth enamel as well as in A-type carbonated apatite exposed to X-rays or to excited oxygen has an orthorhombic character and must be attributed to CO2-. Effectively, the mean values foun...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02409421
更新日期:1981-01-01 00:00:00
abstract::Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-001-1106-y
更新日期:2002-11-01 00:00:00
abstract::The feasibility of two noninvasive methods [dual photon absorptiometry (DPA) and dual energy X-ray absorptiometry (DXA)] for prediction in vivo of local variations of trabecular bone strength within the proximal tibia was evaluated in 14 cadaveric knees. Trabecular bone strength was measured using an osteopenetrometer...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900131
更新日期:1996-10-01 00:00:00
abstract::Aging is associated with an increase in circulating inflammatory factors. One, the cytokine stromal cell-derived factor 1 (SDF-1 or CXCL12), is critical to stem cell mobilization, migration, and homing as well as to bone marrow stem cell (BMSC), osteoblast, and osteoclast function. SDF-1 has pleiotropic roles in bone ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-017-0245-8
更新日期:2017-06-01 00:00:00
abstract::The purpose of this study was to examine the influence of exercise on the serum concentrations of intact parathyroid hormone (PTH). Serum PTH and plasma lactate were measured in 15 well-trained men, 9 long-distance runners and 6 fire-fighters, during two running exercises. Test one consisted of 40-minute treadmill run...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00295947
更新日期:1994-04-01 00:00:00
abstract::The pharmacokinetics and bioavailability of pamidronate were assessed in patients with breast cancer, 6 subjects received the drug intravenously and 7 orally. The initial plasma half-life of pamidronate was short (42 +/- 27 min) and the apparent total plasma clearance was high (471 +/- 298 ml/min). The renal clearance...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555856
更新日期:1991-12-01 00:00:00
abstract::Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane wa...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-020-00793-1
更新日期:2021-01-09 00:00:00
abstract::Abnormal PTH secretion and cell growth in hyperparathyroid tissues are accompanied with reduced expression of Ca2+-sensing receptor (CaR) which plays a key role in Ca-regulated PTH release. In this study, we examined the receptor expression in parathyroid adenomas using specific anti-CaR antibody and investigated rela...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900617
更新日期:1999-03-01 00:00:00
abstract::The solubilization of alkaline phosphatase (AP) from osteoblastic cell membranes obtained from human primary bone cell cultures was studied according to the age and sex of the donors (17 females, 11 males; age range: 2-77 years). Cell membranes were treated by non-ionic (n-octyl beta-D-glucopyranoside, OG), ionic or z...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230050006
更新日期:2000-01-01 00:00:00
abstract::A strategy based on high pressure liquid chromatography (HPLC) techniques for the isolation or a principal amelogenin molecule from a single human dentition is described. A partial sequence (33 residues) for this 24 kDa amelogenin is presented and related to earlier studies of human 5 kDa tyrosine-rich amelogenin poly...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555993
更新日期:1990-08-01 00:00:00
abstract::Prior exposure to PTH markedly decreased the responsiveness of isolated, cultured bone cells to the stimulatory effect of the hormone on cyclic AMP formation. This process of desensitization developed within 30 min, persisted during prolonged incubation of the cells in PTH-free medium, and could not be attributed to e...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02408528
更新日期:1980-01-01 00:00:00
abstract::Condylar cartilages were cultured in the form of organ cultures on top of collagen sponges in medium containing 2% fetal calf serum and were treated with 3.5-350 nM insulin for 6 days. Doses of 175 nM of insulin caused a marked increase (+96%) in DNA synthesis and in proteoglycan production (+74%), features that manif...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00296654
更新日期:1993-04-01 00:00:00
abstract::Genome-wide association studies (GWAS) have demonstrated that genetic variation at the MADS box transcription enhancer factor 2, polypeptide C (MEF2C) locus is robustly associated with bone mineral density, primarily at the femoral neck. MEF2C is a transcription factor known to operate via the Wnt signaling pathway. O...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-013-9824-5
更新日期:2014-04-01 00:00:00
abstract::We examined the role of sleep, growth hormone (GH), and parathyroid hormone [PTH(1-84)] as regulators of the diurnal rhythm of the osteoblastic bone marker, serum osteocalcin (OC). Nine normal subjects were followed with hourly blood sampling during one 24-hour period with normal sleep pattern, and one 24-hour period ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555845
更新日期:1991-12-01 00:00:00
abstract::Pregnancy and lactation-associated osteoporosis (PAO) is a rare condition with little known pathophysiology. Most cases are diagnosed in the third trimester of pregnancy or in the first weeks postpartum, particularly in first pregnancies. Vertebral fractures are most commonly observed and characterised by prolonged se...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-015-0028-z
更新日期:2015-10-01 00:00:00
abstract::This study sought to confirm that osteoblasts of C3H/HeJ (C3H) mice, which have higher differentiation status and bone-forming ability compared to C57BL/6J (B6) osteoblasts, also have a lower apoptosis level and to test whether the higher differentiation status and bone-forming ability of C3H osteoblasts were related ...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-005-0303-5
更新日期:2006-05-01 00:00:00
abstract::Fracture healing is a natural process that recapitulates embryonic skeletal development. In the early phase after fracture, reactive oxygen species (ROS) are produced under inflammatory and ischemic conditions due to vessel injury and soft tissue damage, leading to cell death. Usually, such damage during the course of...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-019-00576-3
更新日期:2019-10-01 00:00:00
abstract::We investigated whether polymorphisms in PTHR1 are associated with bone mineral density (BMD), to determine whether the association of this gene with BMD was due to effects on attainment of peak bone mass or effects on subsequent bone loss. The PTHR1 gene, including its 14 exons, their exon-intron boundaries, and 1,50...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-007-9072-7
更新日期:2007-10-01 00:00:00
abstract::We have examined the effect of the anti-tumor compound taxol, on osteoclastic bone resorption. In the bone slice assay, taxol (0.1-0.001 microM) dose-dependently inhibited bone resorption with an IC50 of 0.08 microM. Osteoclast survival on bone slices was unaffected by 0.01-1 microM taxol, but 10 microM was cytotoxic....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00301951
更新日期:1995-12-01 00:00:00
abstract::The values of in vivo T1 relaxation time (T1) of phosphorus atoms of wrist bone have been measured by phosphorus-31 magnetic resonance spectroscopy (MRS) in 65 menopausal women separated into three groups: (1) age-matched women without any paraclinical or clinical osteoporosis; (2) patients with paraclinical osteoporo...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298583
更新日期:1995-06-01 00:00:00
abstract::The relationship between bisphosphonate-induced bone mineral density (BMD) gains and antifracture efficacy remains to be fully elucidated. Data from two antifracture studies were analyzed. Postmenopausal osteoporotic women received oral (2.5 mg daily, 20 mg intermittent) or intravenous (0.5 mg, 1 mg quarterly) ibandro...
journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00223-010-9403-y
更新日期:2010-10-01 00:00:00