Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques.

Abstract:

:A strategy based on high pressure liquid chromatography (HPLC) techniques for the isolation or a principal amelogenin molecule from a single human dentition is described. A partial sequence (33 residues) for this 24 kDa amelogenin is presented and related to earlier studies of human 5 kDa tyrosine-rich amelogenin polypeptides (TRAPs). A failure to identify amino acid residue #25 (tryptophan in other amelogenins) suggests that this 24 kDa amelogenin is the progenitor of the human TRAP-2 molecule and provides further support for the possibility of several human amelogenin gene products, generated by splice-junction selection, from the single amelogenin gene in the same individual. Alternatively, multiple amelogenins may arise by expression of both the AMELX and AMELY loci.

journal_name

Calcif Tissue Int

authors

Fincham AG,Hu YY,Lau E,Pavlova Z,Slavkin HC,Snead ML

doi

10.1007/BF02555993

subject

Has Abstract

pub_date

1990-08-01 00:00:00

pages

105-11

issue

2

eissn

0171-967X

issn

1432-0827

journal_volume

47

pub_type

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