Abstract：
:The solubilization of alkaline phosphatase (AP) from osteoblastic cell membranes obtained from human primary bone cell cultures was studied according to the age and sex of the donors (17 females, 11 males; age range: 2-77 years). Cell membranes were treated by non-ionic (n-octyl beta-D-glucopyranoside, OG), ionic or zwitterionic detergents, then centrifuged. When OG was used almost all the AP was solubilized. AP activity in supernatant of solubilization was compared to the activity of the suspension before centrifugation. The activity ratio (AR) increased in function of age for subjects between 65 and 74. Neither total nor specific AP activities were influenced by age or sex. Electrophoresis studies showed that the AP released was a GPI (glycosyl phosphatidylinositol)-anchored protein, amphipathic form, with 140 kDa as apparent molecular mass. The activity change of AP in the presence of OG may result from age-related modifications either in the AP structure or in the constituents of the plasma membranes (proteins or phospholipids).
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Bourrat C,Radisson J,Chavassieux P,Azzar G,Roux B,Meunier PJdoi
10.1007/s002230050006subject
Has Abstractpub_date
2000-01-01 00:00:00pages
22-8issue
1eissn
0171-967Xissn
1432-0827pii
CTI-454journal_volume
66pub_type
杂志文章abstract：:The purpose of this study is to evaluate degenerative factors in a postmenopausal patient group and differentiate the influence on bone mineral density (BMD) measurements by dual-energy X-ray absorptiometry (DXA). The patients and methods included an investigation of 144 postmenopausal women (mean 63.3 years) with PA-...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900258
更新日期：1997-05-01 00:00:00
abstract：:The present study examined calcium phosphate precipitation in aqueous suspensions of multilamellar liposomes as a possible in vitro model for matrix vesicle mineralization. Liposomes were prepared by dispersing CHCl3-evaporated thin films of 7:2:1 and 7:1:1 molar mixtures of phosphatidylcholine, dicetyl phosphate, and...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405354
更新日期：1984-07-01 00:00:00
abstract：:Doses of 8, 16 (low), 32, 48, 64 (medium), and 150 (high) mg/kg/day of cortisol were administered to groups of 8 growing rats each during 16 days, and their femurs were then submitted to 3-point bending tests at low strain rate. Low doses had no effect. Medium doses, previously shown to improve calcium (Ca) balance an...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00297297
更新日期：1992-01-01 00:00:00
abstract：:Osteoporosis is commonly associated with estrogen deficiency. However, the mechanisms by which the lack of this hormone causes bone loss are poorly understood. The bone structure of the oral cavity seems to be affected by estrogen deficiency, since a delayed healing process after tooth extraction has been observed aft...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-004-0013-4
更新日期：2005-02-01 00:00:00
abstract：:Formalin fixation of hard tissues alters the levels of some, but not all, of the parameters routinely measured by bone researchers. Although mineral parameters (ash weight, particle size (beta 1/2 002) and Ca/PO4 ratio) and lipid parameters (total lipid content and composition, extractability, and Ca-acidic phospholip...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411262
更新日期：1982-07-01 00:00:00
abstract：:Osteoclastic bone resorption can be induced within the auditory bulla of the Mongolian gerbil by minute increases in air pressure. The objective of this study was to determine the rate at which osteoclasts are induced in areas of resorption and to compare the rate of osteoblast recruitment. Stainless steel tubes were ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556026
更新日期：1989-11-01 00:00:00
abstract：:The dentin-enamel junction (DEJ) plays an important role in preventing crack propagation from enamel into dentin. This function stems from its complex structure and materials properties that are different from either dentin or enamel. The molecular structural differences in both mineral and organic matrix across the D...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9212-8
更新日期：2009-03-01 00:00:00
abstract：:The purpose of this research was to address the following question: How is the solubility of fluoride-pretreated carbonated apatite (CAP) in aqueous acidic media related to the equilibrium solution fluoride and/or the CAP adsorbed fluoride levels? A CAP sample prepared by a precipitation method at 70 degrees C contain...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1069-z
更新日期：2003-03-01 00:00:00
abstract：:There is still controversy over the impact of diabetes control and duration on bone mass and growth parameters in children and adolescents with insulin-dependent diabetes mellitus (IDDM). The aim of this study was to assess bone mineral density (BMD) at axial and appendicular sites, in children with noncomplicated IDD...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900390
更新日期：1998-01-01 00:00:00
abstract：:Osseous tissue develops via two distinctly different processes: endochondral (EC) ossification and intramembranous (IM) ossification. The present study tests the hypothesis that each type of osseous tissue contains unique inducing factors for the promotion of cartilage and bone development. Previous work suggests that...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556258
更新日期：1991-11-01 00:00:00
abstract：:To study the risk of fractures associated with anxiolytics, sedatives, and hypnotics, we conducted a case-control study. Cases were all subjects with any fracture during the year 2000 (n = 124,655). For each case, three controls (n = 373,962) matched on age and gender were randomly drawn from the background population...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9095-0
更新日期：2008-01-01 00:00:00
abstract：:Mandibular first molars from 17-day-old mouse embryos were cultivated in vitro for 10 days using a new organ culture method. This method consisted of using a small glass dish and an agar chamber to slowly float the molars to the gas-medium interface where they were maintained by the surface tension of the medium. The ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555218
更新日期：1986-10-01 00:00:00
abstract：:Osteoporosis is a bone disease leading to an increased fracture risk. It is considered a complex multifactorial genetic disorder with interaction of environmental and genetic factors. As a candidate gene for osteoporosis, we studied vitamin D binding protein (DBP, or group-specific component, Gc), which binds to and t...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9251-9
更新日期：2009-08-01 00:00:00
abstract：:Five years ago we reported results from a cross-sectional study of the effect of nutritional factors on calcium-regulating hormones and bone loss in perimenopausal women. We found an inverse correlation between serum 25-hydroxyvitamin D (25OHD) and immunoreactive parathyroid hormone (PTH), and we postulated that over ...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/BF00334543
更新日期：1992-09-01 00:00:00
abstract：:As tobacco smoking has been identified as a risk factor in the development of osteoporosis, possible deleterious effects of nicotine inhalation on bone mineral density (BMD) and mechanical properties of the femur in female rats were studied. Female Sprague Dawley rats were exposed to nicotine vapour 20 hours a day 5 d...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900692
更新日期：1999-09-01 00:00:00
abstract：:We examined the role of sleep, growth hormone (GH), and parathyroid hormone [PTH(1-84)] as regulators of the diurnal rhythm of the osteoblastic bone marker, serum osteocalcin (OC). Nine normal subjects were followed with hourly blood sampling during one 24-hour period with normal sleep pattern, and one 24-hour period ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555845
更新日期：1991-12-01 00:00:00
abstract：:The aim of our study was to compare the results provided by the measurement of vertebral and femoral bone mineral density (BMD) for assessing the individual risk of osteoporosis as defined by either low BMD and/or rapid bone loss. Vertebral and femoral BMD were measured twice at a mean interval of 21 months in 85 norm...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00310196
更新日期：1993-05-01 00:00:00
abstract：:Targeted disruption of the c-src gene leads to a severe form of osteopetrosis in mice [2]. As the c-src gene is expressed in all tissues and cells tested, we have analyzed fibroblasts from three individuals with malignant, congenital osteopetrosis for the expression of c-src at the protein level. No differences could ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF01352018
更新日期：1993-07-01 00:00:00
abstract：:To determine the relationships among nutrients intake, bone mass, and bone turnover in women we have investigated these issues in a population-based, cross-sectional, observational study in one county in central Sweden. A total of 175 women aged 28-74 at entry to the study were included. Dietary assessment was made by...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298425
更新日期：1995-08-01 00:00:00
abstract：:In the past decade there have been numerous publications reporting a significant and direct relationship between handgrip strength and bone mineral density (BMD) of the proximal femur in older adults. The present report challenges the appropriateness of the methods, and thus the conclusions used in these studies. Spec...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900621
更新日期：1999-04-01 00:00:00
abstract：:In the rat, prolonged administration of the luteinizing, hormone-releasing hormone agonist buserelin (25 micrograms/kg body wt/day s.c.) lowers blood estradiol, raises bone resorption, and induces osteopenia. The present study was undertaken to determine whether withdrawal of buserelin normalizes blood estradiol, slow...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555819
更新日期：1990-01-01 00:00:00
abstract：:1,25(OH)2D3 was found to regulate its own receptor levels via an increase in corresponding mRNA levels in human osteoblast-like osteosarcoma cells (MG-63). In addition, exposure of the cells for 24h to dexamethasone, estradiol, retinoic acid, or triiodothyronine resulted in a dose-dependent accumulation of hVDR mRNA. ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555101
更新日期：1991-01-01 00:00:00
abstract：:We have previously shown that cyclosporin A (CsA) produces high bone remodeling with resorption exceeding formation and loss of bone volume in the rat. This may have important clinical implications where CsA is widely used in organ transplantation. 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) is a bone mineralizing hormone ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555980
更新日期：1990-09-01 00:00:00
abstract：:A 69-year-old man became hypocalcemic under medical observation. The hypocalcemia occurred in the presence of circulating immunoreactive parathyroid hormone (PTH). Common causes of secondary hyperparathyroidism were excluded, as was PTH resistance using PTH infusions. The immunoreactive PTH was examined in detail. PTH...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298499
更新日期：1992-08-01 00:00:00
abstract：:The aim of this study was to investigate bone mineral density (BMD) and bone turnover in patients with primary knee osteoarthritis (KOA) and to compare them with generalized OA (GOA) and nonGOA patients. A total of 88 postmenopausal primary KOA patients were studied. OA was graded by using knee radiographs. BMD of the...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230010068
更新日期：2000-05-01 00:00:00
abstract：:Gnathodiaphyseal dysplasia (GDD; OMIM#166260) is a rare skeletal disorder which is mainly characterized by cemento-osseous lesions in mandibles, bone fragility, bowing and diaphyseal sclerosis of tubular bones. GDD is caused by point mutations in Anoctamin-5 (ANO5); however, the disease mechanisms remain unclear. Here...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00528-x
更新日期：2019-06-01 00:00:00
abstract：:Although radiogenic free radicals are widely used as molecular probes in biological calcified tissues, their nature and their location remain controversial. In the present study we have synthesized and irradiated apatites in which 13CO3(2-) ions substitute hydroxyl or phosphate ions in hydroxyapatite. The 13C-coupling...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The present study reports on the separation of fluid from soft, "cheeselike" enamel of porcine permanent teeth in the secretory stage, and the determination of its chemical composition. The enamel tissues were dissected from mandibles of 5 to 6-month-old piglets and pooled under mineral oil in centrifuge tubes, and th...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555250
更新日期：1987-08-01 00:00:00
abstract：:The aim of this study was to test whether the nitric oxide (NO) donor sodium nitroprusside (SNP) has an effect on mineralization in ATDC5 cells. Mineralization in ATDC5 cell culture was induced by addition of beta-glycerophosphate or inorganic phosphate, visualized by staining precipitated calcium with an alizarin red...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-1233-y
更新日期：2006-03-01 00:00:00
abstract：:A whole-body counter was used to measure the retention and distribution of radioactivity along the longitudinal axis of the body during 10 days after the intravenous injection of 50 microCi of 47Ca. These data and the simultaneous measurements of the serum specific activity allowed us to calculate, by the Bauer-Carlss...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405001
更新日期：1983-01-01 00:00:00
