The enamel fluid in the early secretory stage of porcine amelogenesis: chemical composition and saturation with respect to enamel mineral.

Abstract:

:The present study reports on the separation of fluid from soft, "cheeselike" enamel of porcine permanent teeth in the secretory stage, and the determination of its chemical composition. The enamel tissues were dissected from mandibles of 5 to 6-month-old piglets and pooled under mineral oil in centrifuge tubes, and then centrifuged at 1.9-2.4 X 10(5) g for 1-1.5 hours. The yields of the fluid were 44.8 +/- 2.3 (mean +/- standard error) microliter/g of enamel tissue at 1.9 X 10(5) g, and 53.9 +/- 1.9 microliter/g at 2.4 X 10(5) g. A significant finding was that the total Ca concentration of the enamel fluid (3.9-6.0 X 10(-4) M) was lower than that of porcine serum (2.9 X 10(-3) M), reflecting a distinct, compartmentalized microenvironment, isolated from the circulating blood. Another significant finding was that the ionic calcium activity (5.3 X 10(-5) M) in the enamel fluid was one order of magnitude lower than the total Ca concentration. The averaged results of other determinations were: pH, 7.26; total [P], 3.9 mM; [Mg2+], 0.8 mM; [Na+], 140 mM; [K+], 20 mM; [Cl-], 150 mM; [F-], 5 X 10(-3) mM; and osmolality of the fluid, 312 mosmol/kg H2O (in the same range as that of the serum, 310 mosmol/kg H2O). The apparent electrical unbalance of the analytical data, 8.65 meq excess of positive charges, was ascribed to the presence of HCO3- in the fluid; the computed ionic strength was 164 mM.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Calcif Tissue Int

authors

Aoba T,Moreno EC

doi

10.1007/BF02555250

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

86-94

issue

2

eissn

0171-967X

issn

1432-0827

journal_volume

41

pub_type

杂志文章
  • Changes in heated and in laser-irradiated human tooth enamel and their probable effects on solubility.

    abstract::Enamel of intact human teeth laser irradiated in vitro under certain conditions is known to have less subsurface demineralization than unirradiated enamel on exposure to acid; consequently, the potential use of laser irradiance to reduce caries is apparent. The laser-induced physical and/or chemical changes that cause...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/BF02556711

    authors: Fowler BO,Kuroda S

    更新日期:1986-04-01 00:00:00

  • Impact of detraining on bone loss in former collegiate female gymnasts.

    abstract::Undesirable changes in health-related parameters are thought to occur in retiring female athletes, but this has not been examined in longitudinal studies. The purpose of this study was to examine longitudinal changes in bone mineral density (BMD), body composition, and dietary intake in gymnasts and controls. Nonathle...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-004-0228-4

    authors: Kudlac J,Nichols DL,Sanborn CF,DiMarco NM

    更新日期:2004-12-01 00:00:00

  • Clinical and Radiological Characterization of Patients with Immobilizing and Progressive Stress Fractures in Methotrexate Osteopathy.

    abstract::Methotrexate (MTX) is one of the most commonly prescribed drugs for autoimmune rheumatic diseases. As there is no consensus on its negative effects on bone, the purpose of this investigation was to determine the clinical spectrum of patients with stress fractures due to long-term MTX treatment (i.e., MTX osteopathy). ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-020-00765-5

    authors: Rolvien T,Jandl NM,Stürznickel J,Beil FT,Kötter I,Oheim R,Lohse AW,Barvencik F,Amling M

    更新日期:2021-02-01 00:00:00

  • Molecular structure at the bone-implant interface: a vibrational spectroscopic characterization.

    abstract::Test implant plates surgically retrieved from distal femurs of dogs were studied by Raman spectroscopy in order to characterize the bone-implant interface. The implant surface consisted of phosphate mineral, plasma sprayed on a titanium substrate. On the basis of its spectroscopic signature, the phosphate mineral of b...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556157

    authors: Walters MA,Blumenthal NC,Leung Y,Wang Y,Ricci JL,Spivak JM

    更新日期:1991-05-01 00:00:00

  • Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.

    abstract::We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activatio...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-001-1110-2

    authors: Kikuyama A,Fukuda K,Mori S,Okada M,Yamaguchi H,Hamanishi C

    更新日期:2002-09-01 00:00:00

  • Structural asymmetry between the hips and its relation to experimental fracture type.

    abstract::Experimental analysis with paired femurs provides the opportunity to study within-person differences in fracture type and associated structural side differences. We hypothesized that different fracture types in the hips of a subject are associated with structural asymmetry. Bone mineral density (BMD) and structural me...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-010-9388-6

    authors: Thevenot J,Pulkkinen P,Kuhn V,Eckstein F,Jämsä T

    更新日期:2010-09-01 00:00:00

  • The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures.

    abstract::Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, is catabolic to bone. The increase in the RANKL/OPG ratio in response to continuous PTH, resulting in increased osteoclastogenesis, is well established. However, the effects of prolonged PTH exposure on key regulators o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-016-0176-9

    authors: Houston DA,Myers K,MacRae VE,Staines KA,Farquharson C

    更新日期:2016-11-01 00:00:00

  • Effect of a hypergravity environment on cortical bone elasticity in rats.

    abstract::There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900111

    authors: Kohles SS,Bowers JR,Vailas AC,Vanderby R Jr

    更新日期:1996-09-01 00:00:00

  • Ossification centers of human femur.

    abstract::The purpose of the present work was to investigate the appearance of ossification centers in human femur by Dual-energy X-ray Absorptiometry (DXA), comparing densitometric data with morphogenetic events. Posteroanterior scans were performed on 31 dried femora (from 11.5 weeks of conceptual age to 1 year of postnatal l...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/pl00005841

    authors: Panattoni GL,D'Amelio P,Di Stefano M,Isaia GC

    更新日期:2000-04-01 00:00:00

  • Prevention of osteopenia induced with a gonadotropin-releasing hormone agonist in rats.

    abstract::We investigated the effects of conjugated estrogens as an add-back replacement drug, incadronate sodium as a bisphosphonate, and alfacalcidol as a vitamin D(3) analog on femoral bone mineral density (BMD) and bone mineral content (BMC) in female rats chronically treated with the gonadotropin-releasing hormone (GnRH) a...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900675

    authors: Sakamoto S,Sassa S,Mitamura T,Kudo H,Suzuki S,Yoshimura S,Zhou Y,Kikuchi T,Shinoda H

    更新日期:1999-08-01 00:00:00

  • Factors influencing the enhanced hypocalcemic action of liposome-entrapped calcitonin.

    abstract::The mechanism whereby liposomal entrapment enhances the hypocalcemic effect of calcitonin (CT) was evaluated in the rat. Phosphatidylcholine (PC) and PC plus cholesterol (PCC) preparations of large multilammelar vesicles (MLV) and small unilammelar vesicles (SUV) were used to entrap human calcitonin (hCT). The effect ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555889

    authors: Fukunaga M,Miller MM,Deftos LJ

    更新日期:1990-12-01 00:00:00

  • Glucose transport system in UMR-106-01 osteoblastic osteosarcoma cells: regulation by insulin.

    abstract::Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556657

    authors: Ituarte EA,Halstead LR,Iida-Klein A,Ituarte HG,Hahn TJ

    更新日期:1989-07-01 00:00:00

  • X-ray diffraction of the calcified tissues in Polypterus.

    abstract::Pyrolyzed scales, fin spines, and bone from the ray-finned bony fish Polypterus (Actinopterygii) showed two mineral phases on X-ray diffraction: hydroxyapatite (HA), Ca5(PO4)3OH, and whitlockite, Ca3(PO4)2. The ratio of HA/whitlockite varied with the structure (scale, spine, bone) within each individual fish. The rela...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02441216

    authors: Meinke DK,Skinner HC,Thomson KS

    更新日期:1979-08-24 00:00:00

  • Diet, bone mass, and osteocalcin: a cross-sectional study.

    abstract::To determine the relationships among nutrients intake, bone mass, and bone turnover in women we have investigated these issues in a population-based, cross-sectional, observational study in one county in central Sweden. A total of 175 women aged 28-74 at entry to the study were included. Dietary assessment was made by...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00298425

    authors: Michaëlsson K,Holmberg L,Mallmin H,Wolk A,Bergström R,Ljunghall S

    更新日期:1995-08-01 00:00:00

  • Influence of Teriparatide and Ibandronate on Cortical Bone in New Zealand White Rabbits: A HR-QCT Study.

    abstract::Teriparatide (TPTD) is known to increase the cortical thickness and porosity. The purpose of the present study was to determine whether switching from TPTD to ibandronate (IBN) would be useful for improving cortical bone parameters as assessed using high-resolution quantitative computed tomography (HR-QCT) analyses in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-016-0180-0

    authors: Iwamoto J,Seki A,Nango N

    更新日期:2016-11-01 00:00:00

  • Strontium and bone nanostructure in normal and ovariectomized rats investigated by scanning small-angle X-ray scattering.

    abstract::The effect of SrCl(2) treatment on bone nanostructure in a rat ovariectomy model was studied using scanning small-angle X-ray scattering (sSAXS). Twelve 6-month-old female Wistar rats were used. Six animals were ovariectomized (+ovx) and six were left intact after sham surgery (-ovx). Six animals, three +ovx and three...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-010-9341-8

    authors: Bünger MH,Oxlund H,Hansen TK,Sørensen S,Bibby BM,Thomsen JS,Langdahl BL,Besenbacher F,Pedersen JS,Birkedal H

    更新日期:2010-04-01 00:00:00

  • A Polymorphism in a gene encoding Perilipin 4 is associated with height but not with bone measures in individuals from the Framingham Osteoporosis Study.

    abstract::There is increasing interest in identifying new pathways and candidate genes that confer susceptibility to osteoporosis. There is evidence that adipogenesis and osteogenesis may be related, including a common bone marrow progenitor cell for both adipocytes and osteoblasts. Perilipin 1 (PLIN1) and Perilipin 4 (PLIN4) a...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-011-9552-7

    authors: Cusano NE,Kiel DP,Demissie S,Karasik D,Adrienne Cupples L,Corella D,Gao Q,Richardson K,Yiannakouris N,Ordovas JM

    更新日期:2012-02-01 00:00:00

  • Nasal human calcitonin for tumor-induced hypercalcemia.

    abstract::We treated four hypercalcemic cancer patients by nasal hCT, 3 x 2 mg daily, which has been reported to be active in Paget's disease at lower doses. Only one patient became normocalcemic and mean (+/- SEM) calcium levels fell from 11.6 +/- 0.2 mg/dl before therapy to 10.7 +/- 0.6 mg/dl 2-3 days after starting hCT. The ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00296210

    authors: Dumon JC,Magritte A,Body JJ

    更新日期:1992-07-01 00:00:00

  • Increase in vertebral fracture risk in postmenopausal women using omeprazole.

    abstract::Proton pump inhibitors are taken by millions of patients for prevention and treatment of gastroesophageal diseases. Case-control studies have suggested that use of omeprazole is associated with an increased risk of hip fractures. The aim of this prospective study was to assess the risk of vertebral fractures in postme...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s00223-008-9188-4

    authors: Roux C,Briot K,Gossec L,Kolta S,Blenk T,Felsenberg D,Reid DM,Eastell R,Glüer CC

    更新日期:2009-01-01 00:00:00

  • Regulation of hydroxyapatite formation by gelatin and type I collagen gels.

    abstract::Calcium and phosphate were allowed to diffuse into gelatin and Type I collagen gels which were then cut into slices and analyzed for ion concentrations. Solutions of calcium and phosphate were then prepared, with ion concentrations equivalent to the highest levels in the slices, and mixed together, whereupon a rapid a...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556459

    authors: Blumenthal NC,Cosma V,Gomes E

    更新日期:1991-06-01 00:00:00

  • Sensitivity of dual-photon absorptiometry in spinal osteoporosis.

    abstract::Lumbar spine bone mass and density were measured with Dual photon absorptiometry (DPA) in 60 patients with crush fractures and 60 age-matched normal women. Short-term reproducibility of bone mineral density (BMD) was 1.3% in normal women and 2.5% in osteoporotic women; long-term reproducibility in normal women was 2.2...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02553274

    authors: Pouilles JM,Tremollieres F,Louvet JP,Fournie B,Morlock G,Ribot C

    更新日期:1988-12-01 00:00:00

  • Regulation of osteoclasts by calcitonin and amphiphilic calcitonin conjugates: role of cytosolic calcium.

    abstract::The peptide hormone calcitonin is a potent inhibitor of osteoclastic resorption, but it is unstable and poorly absorbed following oral administration. Conjugates of salmon calcitonin covalently linked to low-molecular-weight amphiphilic polymers show improved stability and absorption. The purpose of this study was to ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-002-1051-4

    authors: Komarova SV,Shum JB,Paige LA,Sims SM,Dixon SJ

    更新日期:2003-09-01 00:00:00

  • Prospects for Therapies in Osteoarthritis.

    abstract::Osteoarthritis (OA) is a chronic, debilitating disease affecting millions of people worldwide. Management of OA involves pharmacological and non-pharmacological approaches. Conventional pharmacological treatments have limited efficacy and are associated with a number of side-effects, restricting the number of patients...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-020-00672-9

    authors: Ghouri A,Conaghan PG

    更新日期:2020-02-13 00:00:00

  • Functional cooperativity between osteoblast transcription factors: evidence for the importance of subnuclear macromolecular complexes?

    abstract::The accompanying article by Drs. Lian and Stein describes current thinking on how genes are organized in the nucleus and suggests that subnuclear localization is critical for the control of gene expression in bone. In particular, it is proposed that a major function of the osteoblast transcription factor, Runx2, is to...

    journal_title:Calcified tissue international

    pub_type: 评论,杂志文章,评审

    doi:10.1007/s00223-002-1053-2

    authors: Franceschi RT

    更新日期:2003-06-01 00:00:00

  • Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar tooth in vitro.

    abstract::Mandibular first molars of 17-day-old mouse embryos were cultured in vitro to examine the histological effects of various concentrations of parathyroid hormone (PTH) on odontogenesis of the molars. PTH did not affect the cytodifferentiation of mesenchymal cells into preodontoblasts but inhibited that of preodontoblast...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555728

    authors: Sakakura Y

    更新日期:1987-01-01 00:00:00

  • Integrated therapies for osteoporosis and sarcopenia: from signaling pathways to clinical trials.

    abstract::Sarcopenia and osteoporosis are two sides of the same coin. They represent different aspects of the same age-related process of musculoskeletal atrophy and together culminate in falls, fractures, deconditioning, and increased mortality in older individuals. However, the current therapeutic approach to the prevention o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-015-9956-x

    authors: Girgis CM

    更新日期:2015-03-01 00:00:00

  • Optimal conditions for Ca-acidic phospholipid-PO4 formation.

    abstract::Calcium-acidic phospholipid-phosphate complexes (Ca-PL-PO4) cause hydroxyapatite (HA) deposition in vitro. The acidic phospholipids--phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG)--all form Ca-PL-PO4 complexes: Nonacidic phospholipids--sphingomyelin (SPL...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:

    authors: Boskey AL,Posner AS

    更新日期:1982-01-01 00:00:00

  • Effect of ascorbic acid on protein synthesis and collagen hydroxylation in continuous flow organ cultures of adult mouse periodontal tissues.

    abstract::A continuous flow organ culture system (CFCS) was used to determine the effect of ascorbic acid on the synthesis of collagen and noncollagenous protein by bone of the alveolar process and periodontal ligament in organ cultures of adult mouse periodontium. For the last 24 h of 2 day cultures, 5 microCi/ml 3H-proline wa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02411276

    authors: Sodek J,Feng J,Yen EH,Melcher AH

    更新日期:1982-07-01 00:00:00

  • Phosphate regulates the stability of skeletal alkaline phosphatase activity in human osteosarcoma (SaOS-2) cells without equivalent effects on the level of skeletal alkaline phosphatase immunoreactive protein.

    abstract::Inorganic phosphate (P(i)) can regulate the level of skeletal alkaline phosphatase (ALP) activity in human osteoblast-like cells, but not by means of changes in transcription or release from the cell surface. The current studies were intended to determine whether (1) P(i) affected the inactivation of ALP activity in h...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00302073

    authors: Farley JR

    更新日期:1995-11-01 00:00:00

  • Altered Bone Remodeling in Psoriatic Disease: New Insights and Future Directions.

    abstract::Psoriatic arthritis (PsA) is an inflammatory rheumatic disorder that occurs in patients with psoriasis and predominantly affects musculoskeletal structures, skin, and nails. The etiology of PsA is not well understood but evidence supports an interplay of genetic, immunologic, and environmental factors which promote pa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-017-0380-2

    authors: Paine A,Ritchlin C

    更新日期:2018-05-01 00:00:00