当前位置： SCI文献检索 > CALCIFIED TISSUE INTERNATIONAL期刊下所有文献 > Glucose transport system in UMR-106-01 osteoblastic osteosarcoma cells: regulation by insulin.

Glucose transport system in UMR-106-01 osteoblastic osteosarcoma cells: regulation by insulin.

Abstract：

:Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on glucose metabolism in osteoblast-like cells have not been defined. We therefore characterized 2-deoxy-D-glucose (2-DG) transport in UMR-106-01 rat osteoblastic osteosarcoma cells and examined its regulation by insulin. 2-DG (0.1 mM) uptake was shown to be linear with time over 45 minutes, temperature-sensitive, and inhibited by phloridzin. Competitive inhibition studies against other hexoses demonstrated a transport system stereospecificity for 2-DG similar to that previously demonstrated in fat and muscle cells. Kinetic analysis of 15 minute 2-DG uptake at 25 degrees C demonstrated a saturable transport mechanism with a Km (1.9 mM) similar to that observed for 2-DG transport in other tissues. Insulin stimulated 2-DG transport in a dose-related manner, with significant stimulation observed at 0.5 nM and maximal effect observed at 50 nM insulin. The stimulatory effect of insulin was reversibly inhibited by cytochalasin B (50 microM). Insulin stimulation of 2-DG transport was associated with a 1.7-fold increase in Vmax, while Km remained constant. When insulin effects on glucose transport were inhibited by the addition of 5 mM phloridzin, stimulatory effects on DNA and collagen synthesis were diminished, suggesting that stimulation of glucose transport may play a role in insulin effects on replication and function in osteoblast-like cells.

journal_name

Calcif Tissue Int

journal_title

Calcified tissue international

authors

Ituarte EA,Halstead LR,Iida-Klein A,Ituarte HG,Hahn TJ

doi

10.1007/BF02556657

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

27-33

issue

1

eissn

0171-967X

issn

1432-0827

journal_volume

45

pub_type

杂志文章

相关文献

CALCIFIED TISSUE INTERNATIONAL文献大全
  • Melorheostosis and Osteopoikilosis: A Review of Clinical Features and Pathogenesis.

    abstract：:Melorheostosis is an exceptionally rare sclerosing hyperostosis that typically affects the appendicular skeleton in a limited segmental fashion. It occasionally occurs on a background of another benign generalised sclerosing bone condition, known as osteopoikilosis caused by germline mutations in LEMD3, encoding the i...

    journal_title：Calcified tissue international

    pub_type： 杂志文章,评审

    doi：10.1007/s00223-019-00543-y

    authors： Wordsworth P,Chan M

    更新日期：2019-05-01 00:00:00

  • Determination of histamine and polyamines in calcified tissues of mice: contribution of mast cells and histidine decarboxylase to the amount of histamine in the bone.

    abstract：:A simple method for determining histamine and polyamines in various tissues was devised. The method, however, could not be applied to calcified tissues, because the high concentration of Ca2+ in the extract interferes with the chromatographic separation of these amines. By treating the extracts from calcified tissues ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF00296220

    authors： Endo Y,Kikuchi T,Nakamura M,Shinoda H

    更新日期：1992-07-01 00:00:00

  • Effects of loading rate on strength of the proximal femur.

    abstract：:Results from previous quasi-static mechanical tests indicate that femurs from elderly subjects fail in vitro at forces 50% below those available in a fall from standing height. However, bone is a rate-dependent material, and it is not known whether this imbalance is present at rates of loading which occur in a fall. B...

    journal_title：Calcified tissue international

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF00310169

    authors： Courtney AC,Wachtel EF,Myers ER,Hayes WC

    更新日期：1994-07-01 00:00:00

  • Impaired bone health in inflammatory bowel disease: a case-control study in 80 pediatric patients.

    abstract：:Previous studies have indicated that children with inflammatory bowel disease (IBD) may not achieve optimal bone mass. We evaluated the skeletal characteristics in children and adolescents with IBD. This cross-sectional cohort study comprised 80 IBD patients (median age 14.9 years, range 5-20) with a median disease du...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-012-9617-2

    authors： Laakso S,Valta H,Verkasalo M,Toiviainen-Salo S,Viljakainen H,Mäkitie O

    更新日期：2012-08-01 00:00:00

  • The small bovine amelogenin LRAP fails to rescue the amelogenin null phenotype.

    abstract：:Amelogenins are the most abundant secreted proteins in developing dental enamel. These evolutionarily-conserved proteins have important roles in enamel mineral formation, as mutations within the amelogenin gene coding region lead to defects in enamel thickness or mineral structure. Because of extensive alternative spl...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-002-0036-7

    authors： Chen E,Yuan ZA,Wright JT,Hong SP,Li Y,Collier PM,Hall B,D'Angelo M,Decker S,Piddington R,Abrams WR,Kulkarni AB,Gibson CW

    更新日期：2003-11-01 00:00:00

  • Osteonecrosis in a chemically induced rat model of human hemolytic disorders associated with thrombosis--a new model for avascular necrosis of bone.

    abstract：:Bone injury occurs in human hemolytic disorders associated with thrombosis, such as beta-thalassemia and sickle cell disease. Exposure of rats to 2-butoxyethanol (BE) has been associated with hemolytic anemia, disseminated thrombosis, and infarction in multiple organs including bone. This rat model apparently mimics a...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-003-0068-7

    authors： Shabat S,Nyska A,Long PH,Goelman G,Abramovitch R,Ezov N,Levin-Harrus T,Peddada S,Redlich M,Yedgar S,Nyska M

    更新日期：2004-03-01 00:00:00

  • Developing antler, a model for endocrine regulation of bone growth. Concentration gradient of T3, T4, and alkaline phosphatase in the antler, jugular, and the saphenous veins.

    abstract：:Thyroxine (T4), triiodothyronine (T3), and alkaline phosphatase (AP) were assayed monthly in white-tailed deer plasma obtained from the antler (A), jugular (J), and the saphenous (S) veins during the period of antler growth and the period of mineralization. The concentrations of T3, T4, and AP in the J and S were not ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02555129

    authors： Bubenik GA,Sempere AJ,Hamr J

    更新日期：1987-07-01 00:00:00

  • The inhibitory effects of ATP, ADP, and AMP on the rate of dissolution of calcium hydroxyapatite.

    abstract：:ATP is as effective as inorganic pyrophosphate in retarding the rate of dissolution of calcium hydroxyapatite at pH 7, ADP is less effective, and AMP is without effect. The inhibition is due to the adsorption of ATP and ADP units on the crystal surface. ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02405386

    authors： Christoffersen MR,Christoffersen J

    更新日期：1984-12-01 00:00:00

  • Calcification inhibitors and Wnt signaling proteins are implicated in bovine artery smooth muscle cell calcification in the presence of phosphate and vitamin D sterols.

    abstract：:Administration of active vitamin D sterols to treat secondary hyperparathyroidism in patients with chronic kidney disease receiving dialysis has been associated with elevated serum calcium and phosphorus levels, which may lead to increased risk of vascular calcification. However, calcimimetics, by binding to the parat...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-006-0126-z

    authors： Shalhoub V,Shatzen E,Henley C,Boedigheimer M,McNinch J,Manoukian R,Damore M,Fitzpatrick D,Haas K,Twomey B,Kiaei P,Ward S,Lacey DL,Martin D

    更新日期：2006-12-01 00:00:00

  • Regulation of hydroxyapatite formation by gelatin and type I collagen gels.

    abstract：:Calcium and phosphate were allowed to diffuse into gelatin and Type I collagen gels which were then cut into slices and analyzed for ion concentrations. Solutions of calcium and phosphate were then prepared, with ion concentrations equivalent to the highest levels in the slices, and mixed together, whereupon a rapid a...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02556459

    authors： Blumenthal NC,Cosma V,Gomes E

    更新日期：1991-06-01 00:00:00

  • Increase in tartrate-resistant acid phosphatase of bone at the early stage of ascorbic acid deficiency in the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat.

    abstract：:The effect of ascorbic acid deficiency on bone metabolism was evaluated using the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat model. Ascorbic acid (Asc)-deficient rats gained body weight in a manner similar to Asc-supplemented rats (control) during 3 weeks, but began to lose weight during the 4th week o...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-002-2040-3

    authors： Goto A,Tsukamoto I

    更新日期：2003-08-01 00:00:00

  • Parathyroid hormone as a calcium ionophore in bone cells: tests of specificity.

    abstract：:Parathyroid Hormone (PTH) stimulates calcium entry into bone cells in vitro, and this effect has been proposed to mediate the actions of PTH on bone. Using cells harvested from infant rat calvaria by collagenase digestion, we evaluated the sensitivity and specificity of this phenomenon. Calcium uptake was temperature ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02408543

    authors： Marcus R,Orner FB

    更新日期：1980-01-01 00:00:00

  • Adiponectin and peak bone mass in men: a cross-sectional, population-based study.

    abstract：:Adiponectin, a protein classically known to be secreted by adipocytes, is also secreted by bone-forming cells. Results of previous studies have been contradictory as to whether serum adiponectin and bone mineral density (BMD) are associated. The aim of this study was to investigate a possible association between serum...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-010-9376-x

    authors： Frost M,Abrahamsen B,Nielsen TL,Frystyk J,Flyvbjerg A,Hagen C,Andersen M,Brixen K

    更新日期：2010-07-01 00:00:00

  • PTHR1 polymorphisms influence BMD variation through effects on the growing skeleton.

    abstract：:We investigated whether polymorphisms in PTHR1 are associated with bone mineral density (BMD), to determine whether the association of this gene with BMD was due to effects on attainment of peak bone mass or effects on subsequent bone loss. The PTHR1 gene, including its 14 exons, their exon-intron boundaries, and 1,50...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-007-9072-7

    authors： Vilariño-Güell C,Miles LJ,Duncan EL,Ralston SH,Compston JE,Cooper C,Langdahl BL,Maclelland A,Pols HA,Reid DM,Uitterlinden AG,Steer CD,Tobias JH,Wass JA,Brown MA

    更新日期：2007-10-01 00:00:00

  • Characterization of the bone phenotype in ClC-7-deficient mice.

    abstract：:Mice deficient in the chloride channel ClC-7, which is likely involved in acidification of the resorption lacuna, display severe osteopetrosis. To fully characterize the osteopetrotic phenotype, the phenotypes of osteoclasts and osteoblasts were evaluated. ClC-7(-/-) mice and their corresponding wild-type littermates ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-008-9185-7

    authors： Neutzsky-Wulff AV,Karsdal MA,Henriksen K

    更新日期：2008-12-01 00:00:00

  • Radioimmunoassay for human osteocalcin using an antibody raised against the synthetic human (h37-49) sequence.

    abstract：:Radioiodination of synthetic human 37-49 osteocalcin requires optimal labeling conditions in order to obtain a maximum of mono- and di-iodinated tracer with little contamination by tri- and tetra-iodinated products or "radio-damage." The antibody raised against osteocalcin(h37-49) had the highest affinity for the C-te...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02555196

    authors： Jüppner H,Schettler T,Giebel G,Wenner S,Hesch RD

    更新日期：1986-11-01 00:00:00

  • Bone densitometry in Canadian children 8-17 years of Age.

    abstract：:Normative bone mineral density (BMD) and bone mineral content (BMC) values for the total body (TB), proximal femur (PF), and antero-posterior lumbar spine (LS) were obtained from a large cross-sectional sample of children and adolescents who were 8-17 years of age. There were 977 scans for the TB, 892 for the PF, and ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s002239900138

    authors： Faulkner RA,Bailey DA,Drinkwater DT,McKay HA,Arnold C,Wilkinson AA

    更新日期：1996-11-01 00:00:00

  • Role of oestrogen in the development of osteoporosis.

    abstract：:The evidence now strongly supports a central role for oestrogen in the maintenance of the normal female skeleton. Loss of endogenous oestrogen leads to a period of accelerated bone loss which can be prevented with appropriate oestrogen replacement. The route of administration is not important provided adequate dosages...

    journal_title：Calcified tissue international

    pub_type： 杂志文章,评审

    doi：10.1007/BF02555090

    authors： Hillard TC,Stevenson JC

    更新日期：1991-01-01 00:00:00

  • The effect of menopause on biochemical markers and ultrasound densitometry in healthy females.

    abstract：:Urinary pyridinoline (pyr) and deoxypyridinoline (dpyr) are new markers for bone resorption, and serum osteocalcin reportedly indicates osteoblastic activity. Recently, a new ultrasound bone densitometer instrument has been developed that measures ultrasonic properties of the os calcis, namely, the speed of sound (SOS...

    journal_title：Calcified tissue international

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF00298555

    authors： Kawana K,Kushida K,Takahashi M,Ohishi T,Denda M,Yamazaki K,Inoue T

    更新日期：1994-12-01 00:00:00

  • Structural analysis of high resolution in vitro MR images compared to stained grindings.

    abstract：:The recent advancement of high resolution magnetic resonance imaging has opened up new avenues for the determination of structural characteristics of the trabecular network, which may significantly improve the diagnosis of osteoporosis. An analysis of the calcaneus in healthy women has shown similar age-related change...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s002230001168

    authors： Engelke K,Hahn M,Takada M,Vogel M,Ouyang X,Delling G,Genant HK

    更新日期：2001-03-01 00:00:00

  • Fine powdering exposes the mineral-protected collagen of bone to protease digestion.

    abstract：:The method of heat-denaturation and trypsin digestion was used to dissect bone biochemically into mineral-protected and mineral-unprotected pools of collagenous matrix. It was found that varying the particle size of the bone powder had a profound effect on the results. Using mature bovine cortical bone, the observed p...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02553750

    authors： Wu JJ,Eyre DR

    更新日期：1988-04-01 00:00:00

  • The effect of intravenous pamidronate on bone mineral density, bone histomorphometry, and parameters of bone turnover in adults with type IA osteogenesis imperfecta.

    abstract：:The type IA osteogenesis imperfecta (OI) phenotype is characterized by multiple fractures, blue sclerae, and minimal skeletal deformity without dentinogenesis imperfecta. The object of this study was to determine the effect of treatment with intravenous pamidronate (30 mg) every 3 months on bone density and bone histo...

    journal_title：Calcified tissue international

    pub_type： 临床试验,杂志文章

    doi：10.1007/s00223-001-1055-5

    authors： Shapiro JR,McCarthy EF,Rossiter K,Ernest K,Gelman R,Fedarko N,Santiago HT,Bober M

    更新日期：2003-02-01 00:00:00

  • Maximum grip strength is not related to bone mineral density of the proximal femur in older adults.

    abstract：:In the past decade there have been numerous publications reporting a significant and direct relationship between handgrip strength and bone mineral density (BMD) of the proximal femur in older adults. The present report challenges the appropriateness of the methods, and thus the conclusions used in these studies. Spec...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s002239900621

    authors： Foley KT,Owings TM,Pavol MJ,Grabiner MD

    更新日期：1999-04-01 00:00:00

  • Effects of aluminum(III), chromium(III), and iron(III) on the rate of dissolution of calcium hydroxyapatite crystals in the absence and presence of the chelating agent desferrioxamine.

    abstract：:Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02555127

    authors： Christoffersen MR,Thyregod HC,Christoffersen J

    更新日期：1987-07-01 00:00:00

  • Fluoride absorption: the influence of gastric acidity.

    abstract：:The influence of gastric acidity on the absorption of intragastrically administered fluoride was investigated in rats. Intact animals were pretreated with atropine or cimetidine to reduce gastric acid secretion or were given fluoride in NaHCO3 to reduce the acidity of the gastric contents. Compared with pentagastrin-t...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02405334

    authors： Whitford GM,Pashley DH

    更新日期：1984-05-01 00:00:00

  • X-ray diffraction of the calcified tissues in Polypterus.

    abstract：:Pyrolyzed scales, fin spines, and bone from the ray-finned bony fish Polypterus (Actinopterygii) showed two mineral phases on X-ray diffraction: hydroxyapatite (HA), Ca5(PO4)3OH, and whitlockite, Ca3(PO4)2. The ratio of HA/whitlockite varied with the structure (scale, spine, bone) within each individual fish. The rela...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02441216

    authors： Meinke DK,Skinner HC,Thomson KS

    更新日期：1979-08-24 00:00:00

  • Calcium kinetics in glycogen storage disease type 1a.

    abstract：:Glycogen storage disease type 1a (Von Gierke's disease) is one of the more common glycogen storage diseases (GSD). GSD 1a patients can have severe idiopathic osteopenia, often beginning at a young age. Since calcium tracer studies offer a sensitive probe of the bone microenvironment and of calcium deposition, kinetics...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF00369209

    authors： Goans RE,Weiss GH,Vieira NE,Sidbury JB,Abrams SA,Yergey AL

    更新日期：1996-12-01 00:00:00

  • Bone Matrix Composition Following PTH Treatment is Not Dependent on Sclerostin Status.

    abstract：:Sclerostin and parathyroid hormones are strong negative and positive regulators of bone formation, respectively. The anabolic response induced by intermittent (iPTH) treatment is sclerostin status-dependent. However, the interaction between sclerostin and iPTH at the matrix level is unknown. The goal of the current st...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/s00223-015-0074-6

    authors： Ross RD,Mashiatulla M,Robling AG,Miller LM,Sumner DR

    更新日期：2016-02-01 00:00:00

  • Intestinal lactase activity and calcium absorption in the aging female with osteoporosis.

    abstract：:In 11 postmenopausal women with histologically proven osteoporosis and normal lactose tolerance, a direct correlation between calcium absorption and intestinal lactase activity was observed. The results suggest that varying degrees of relative deficiencies in intestinal lactase might contribute to the graded decrease ...

    journal_title：Calcified tissue international

    pub_type： 杂志文章

    doi：10.1007/BF02557688

    authors： Pacifici R,Droke D,Avioli LV

    更新日期：1985-01-01 00:00:00

  • Fatigue microdamage as an essential element of bone mechanics and biology.

    abstract：:The fossil record shows that bone remodeling has existed since the earliest large vertebrates became weight-bearing on land, but the functions of remodeling have long been debated. The principal protagonists in this debate have been those favoring a mechanical function and those asserting that remodeling serves to mov...

    journal_title：Calcified tissue international

    pub_type： 杂志文章,评审

    doi：10.1007/s00223-002-1059-9

    authors： Martin RB

    更新日期：2003-08-01 00:00:00